UV Spectrophotometric method for determination of posaconazole: comparison to HPLC
The aim of this work was to develop a simple, fast and reproducible spectrophotometric method for the analysis of posaconazole in raw material. The established conditions were: methanol as extracting solvent, detection wavelength of 260 nm, Shimadzu double beam spectrophotometer 1800 model with 1 cm...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
São Paulo State University (UNESP)
2015-10-01
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| Series: | Revista de Ciências Farmacêuticas Básica e Aplicada |
| Subjects: | |
| Online Access: | http://rcfba.fcfar.unesp.br/index.php/ojs/article/view/14 |
| _version_ | 1818609508541267968 |
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| author | Andressa S. Bitencourt Sendy S. Oliveira Andreas S. L. Mendez Cássia V. Garcia |
| author_facet | Andressa S. Bitencourt Sendy S. Oliveira Andreas S. L. Mendez Cássia V. Garcia |
| author_sort | Andressa S. Bitencourt |
| collection | DOAJ |
| description | The aim of this work was to develop a simple, fast and reproducible spectrophotometric method for the analysis of posaconazole in raw material. The established conditions were: methanol as extracting solvent, detection wavelength of 260 nm, Shimadzu double beam spectrophotometer 1800 model with 1 cm quartz cells. Linearity was demonstrated in the concentration range of 5.0 a 25.0 µg/mL (r = 0.9999). Reproducibility and intermediate precision were confirmed by low RSD values (0.49 to 0.82%). Accuracy, evaluated through recovery test, was adequate, with 98.20% of mean recovery. Specificity and robustness were also demonstrated. The mean amount found for samples was 100.82%. The proposed method was considered suitable for the intended purpose, mainly in routine analysis of quality control laboratories. When compared to the previously developed HPLC, no statistical difference was observed, what made the UV spectrophotometric method a reliable alternative. |
| first_indexed | 2024-12-16T14:59:39Z |
| format | Article |
| id | doaj.art-11d183724fa3440591aa7890536a8855 |
| institution | Directory Open Access Journal |
| issn | 1808-4532 2179-443X |
| language | English |
| last_indexed | 2024-12-16T14:59:39Z |
| publishDate | 2015-10-01 |
| publisher | São Paulo State University (UNESP) |
| record_format | Article |
| series | Revista de Ciências Farmacêuticas Básica e Aplicada |
| spelling | doaj.art-11d183724fa3440591aa7890536a88552022-12-21T22:27:20ZengSão Paulo State University (UNESP)Revista de Ciências Farmacêuticas Básica e Aplicada1808-45322179-443X2015-10-0136414UV Spectrophotometric method for determination of posaconazole: comparison to HPLCAndressa S. BitencourtSendy S. OliveiraAndreas S. L. MendezCássia V. GarciaThe aim of this work was to develop a simple, fast and reproducible spectrophotometric method for the analysis of posaconazole in raw material. The established conditions were: methanol as extracting solvent, detection wavelength of 260 nm, Shimadzu double beam spectrophotometer 1800 model with 1 cm quartz cells. Linearity was demonstrated in the concentration range of 5.0 a 25.0 µg/mL (r = 0.9999). Reproducibility and intermediate precision were confirmed by low RSD values (0.49 to 0.82%). Accuracy, evaluated through recovery test, was adequate, with 98.20% of mean recovery. Specificity and robustness were also demonstrated. The mean amount found for samples was 100.82%. The proposed method was considered suitable for the intended purpose, mainly in routine analysis of quality control laboratories. When compared to the previously developed HPLC, no statistical difference was observed, what made the UV spectrophotometric method a reliable alternative.http://rcfba.fcfar.unesp.br/index.php/ojs/article/view/14posaconazole. uv spectrophotometry. quality control. |
| spellingShingle | Andressa S. Bitencourt Sendy S. Oliveira Andreas S. L. Mendez Cássia V. Garcia UV Spectrophotometric method for determination of posaconazole: comparison to HPLC Revista de Ciências Farmacêuticas Básica e Aplicada posaconazole. uv spectrophotometry. quality control. |
| title | UV Spectrophotometric method for determination of posaconazole: comparison to HPLC |
| title_full | UV Spectrophotometric method for determination of posaconazole: comparison to HPLC |
| title_fullStr | UV Spectrophotometric method for determination of posaconazole: comparison to HPLC |
| title_full_unstemmed | UV Spectrophotometric method for determination of posaconazole: comparison to HPLC |
| title_short | UV Spectrophotometric method for determination of posaconazole: comparison to HPLC |
| title_sort | uv spectrophotometric method for determination of posaconazole comparison to hplc |
| topic | posaconazole. uv spectrophotometry. quality control. |
| url | http://rcfba.fcfar.unesp.br/index.php/ojs/article/view/14 |
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