<b>Postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate-based supplement in rats</b>
<p><b>Abstract</b></p> <p>The purpose of this study was: aim 1) compare insulin and leucine serum responses after feeding a novel hydrolyzed whey protein (WPH)-based supplement versus a whey protein isolate (WPI) in rats during the post-absorptive state, and aim 2) to p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2012-06-01
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| Series: | Journal of the International Society of Sports Nutrition |
| Subjects: | |
| Online Access: | http://www.jissn.com/content/9/1/24 |
| _version_ | 1811227614636933120 |
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| author | Toedebusch Ryan G Childs Thomas E Hamilton Shari R Crowley Jan R Booth Frank W Roberts Michael D |
| author_facet | Toedebusch Ryan G Childs Thomas E Hamilton Shari R Crowley Jan R Booth Frank W Roberts Michael D |
| author_sort | Toedebusch Ryan G |
| collection | DOAJ |
| description | <p><b>Abstract</b></p> <p>The purpose of this study was: aim 1) compare insulin and leucine serum responses after feeding a novel hydrolyzed whey protein (WPH)-based supplement versus a whey protein isolate (WPI) in rats during the post-absorptive state, and aim 2) to perform a thorough toxicological analysis on rats that consume different doses of the novel WPH-based supplement over a 30-day period. In male Wistar rats (~250 g, n = 40), serum insulin and leucine concentrations were quantified up to 120 min after one human equivalent dose of a WPI or the WPH-based supplement. In a second cohort of rats (~250 g, n = 20), we examined serum/blood and liver/kidney histopathological markers after 30 days of feeding low (1human equivalent dose), medium (3 doses) and high (6 doses) amounts of the WPH-based supplement. In aim 1, higher leucine levels existed at 15 min after WPH vs. WPI ingestion (p = 0.04) followed by higher insulin concentrations at 60 min (p = 0.002). In aim 2, liver and kidney histopathology/toxicology markers were not different 30 days after feeding with low, medium, high dose WPH-based supplementation or water only. There were no between-condition differences in body fat or lean mass or circulating clinical chemistry markers following the 30-day feeding intervention in aim 2. In comparison to WPI, acute ingestion of a novel WPH-based supplement resulted in a higher transient leucine response with a sequential increase in insulin. Furthermore, chronic ingestion of the tested whey protein hydrolysate supplement appears safe.</p> |
| first_indexed | 2024-04-12T09:45:00Z |
| format | Article |
| id | doaj.art-11d30aff9b53482aacf2d1cbfb23ce7f |
| institution | Directory Open Access Journal |
| issn | 1550-2783 |
| language | English |
| last_indexed | 2024-04-12T09:45:00Z |
| publishDate | 2012-06-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of the International Society of Sports Nutrition |
| spelling | doaj.art-11d30aff9b53482aacf2d1cbfb23ce7f2022-12-22T03:37:58ZengTaylor & Francis GroupJournal of the International Society of Sports Nutrition1550-27832012-06-01912410.1186/1550-2783-9-24<b>Postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate-based supplement in rats</b>Toedebusch Ryan GChilds Thomas EHamilton Shari RCrowley Jan RBooth Frank WRoberts Michael D<p><b>Abstract</b></p> <p>The purpose of this study was: aim 1) compare insulin and leucine serum responses after feeding a novel hydrolyzed whey protein (WPH)-based supplement versus a whey protein isolate (WPI) in rats during the post-absorptive state, and aim 2) to perform a thorough toxicological analysis on rats that consume different doses of the novel WPH-based supplement over a 30-day period. In male Wistar rats (~250 g, n = 40), serum insulin and leucine concentrations were quantified up to 120 min after one human equivalent dose of a WPI or the WPH-based supplement. In a second cohort of rats (~250 g, n = 20), we examined serum/blood and liver/kidney histopathological markers after 30 days of feeding low (1human equivalent dose), medium (3 doses) and high (6 doses) amounts of the WPH-based supplement. In aim 1, higher leucine levels existed at 15 min after WPH vs. WPI ingestion (p = 0.04) followed by higher insulin concentrations at 60 min (p = 0.002). In aim 2, liver and kidney histopathology/toxicology markers were not different 30 days after feeding with low, medium, high dose WPH-based supplementation or water only. There were no between-condition differences in body fat or lean mass or circulating clinical chemistry markers following the 30-day feeding intervention in aim 2. In comparison to WPI, acute ingestion of a novel WPH-based supplement resulted in a higher transient leucine response with a sequential increase in insulin. Furthermore, chronic ingestion of the tested whey protein hydrolysate supplement appears safe.</p>http://www.jissn.com/content/9/1/24DietLeucineWhey hydrolysate |
| spellingShingle | Toedebusch Ryan G Childs Thomas E Hamilton Shari R Crowley Jan R Booth Frank W Roberts Michael D <b>Postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate-based supplement in rats</b> Journal of the International Society of Sports Nutrition Diet Leucine Whey hydrolysate |
| title | <b>Postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate-based supplement in rats</b> |
| title_full | <b>Postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate-based supplement in rats</b> |
| title_fullStr | <b>Postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate-based supplement in rats</b> |
| title_full_unstemmed | <b>Postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate-based supplement in rats</b> |
| title_short | <b>Postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate-based supplement in rats</b> |
| title_sort | b postprandial leucine and insulin responses and toxicological effects of a novel whey protein hydrolysate based supplement in rats b |
| topic | Diet Leucine Whey hydrolysate |
| url | http://www.jissn.com/content/9/1/24 |
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