Differentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ Hybridization
Background & Objective: Soft tissue sarcomas (STS) constitute an uncommon and heterogeneous group of tumors of mesenchymal origin and various cytogenetic abnormalities ranging from distinct genomic rearrangements, such as chromosomal translocations and amplifications, to more intricate rearrange...
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| Format: | Article |
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Iranian Society of Pathology
2017-07-01
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| Series: | Iranian Journal of Pathology |
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| Online Access: | https://ijp.iranpath.org/article_25043_ac79674409a98de9ac72f09068eb05fa.pdf |
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| author | Farhad Shahi Razieh Alishahi Hossein Pashaiefar Isa Jahanzad Naser Kamalian Ardeshir Ghavamzadeh Marjan Yaghmaie |
| author_facet | Farhad Shahi Razieh Alishahi Hossein Pashaiefar Isa Jahanzad Naser Kamalian Ardeshir Ghavamzadeh Marjan Yaghmaie |
| author_sort | Farhad Shahi |
| collection | DOAJ |
| description | Background & Objective: Soft tissue sarcomas (STS) constitute an uncommon and heterogeneous group of tumors of mesenchymal origin and various cytogenetic abnormalities ranging from distinct genomic rearrangements, such as chromosomal translocations and amplifications, to more intricate rearrangements involving multiple chromosomes. Fluorescence in situ hybridization (FISH) can be used to identify these chromosomal translocations and amplifications, and sub classify STS precisely. The current study aimed at investigating the usefulness of FISH, as a diagnostic ancillary aid, to detect cytogenetic abnormalities such as MDM2 (murine double minute 2) amplification and CHOP(C/EBP homologous protein) rearrangement in liposarcoma, as well as SYT (synaptotagmin) rearrangement in synovial sarcoma. Methods: The FISH technique was used to analyze 17 specimens of liposarcoma for MDM2 amplification and CHOP rearrangement, and 10 specimens of synovial sarcoma for SYT rearrangement. The subtypes of liposarcoma and synovial sarcomas were reclassified according to the FISH results and compared with those of the respective histological findings. Results: According to the FISH results in 17 liposarcoma cases, well-differentiated liposarcoma(WDLPS), dedifferentiated liposarcoma (DDLPS), and myxoidliposarcoma (MLPS)subtypes were 41%, 53%, and 6%, respectively. In different subtypes of liposarcoma, a total of 30% mismatches were observed between pathologic and cytogenetic results. According to the histological findings from FISH analysis, SYT rearrangement was found only in three out of 10 (30%) synovial sarcomas. Conclusion: The detection of cytogenetic abnormalities in patients with liposarcoma and synovial sarcoma by FISH technique provides an important objective tool to confirm sarcoma diagnosis and sub classification of specific sarcoma subtypes in such patients. |
| first_indexed | 2024-12-22T00:07:31Z |
| format | Article |
| id | doaj.art-11d39c8cb51345e5a84a4b11a28194b0 |
| institution | Directory Open Access Journal |
| issn | 1735-5303 2345-3656 |
| language | English |
| last_indexed | 2024-12-22T00:07:31Z |
| publishDate | 2017-07-01 |
| publisher | Iranian Society of Pathology |
| record_format | Article |
| series | Iranian Journal of Pathology |
| spelling | doaj.art-11d39c8cb51345e5a84a4b11a28194b02022-12-21T18:45:32ZengIranian Society of PathologyIranian Journal of Pathology1735-53032345-36562017-07-0112320921710.30699/ijp.2017.2504325043Differentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ HybridizationFarhad Shahi0Razieh Alishahi1Hossein Pashaiefar2Isa Jahanzad3Naser Kamalian4Ardeshir Ghavamzadeh5Marjan Yaghmaie6Dept. of Hematology and Medical Oncology, Cancer Research Center, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, IranDept. of Biology, Faculty of Sciences, International Pardis, University of Guilan, Rasht, IranDept. of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDept. of Pathology, Cancer Research Center, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, IranDept. of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research center, Tehran University of Medical Sciences, Tehran, IranHematology, Oncology and Stem Cell Transplantation Research center, Tehran University of Medical Sciences, Tehran, IranBackground & Objective: Soft tissue sarcomas (STS) constitute an uncommon and heterogeneous group of tumors of mesenchymal origin and various cytogenetic abnormalities ranging from distinct genomic rearrangements, such as chromosomal translocations and amplifications, to more intricate rearrangements involving multiple chromosomes. Fluorescence in situ hybridization (FISH) can be used to identify these chromosomal translocations and amplifications, and sub classify STS precisely. The current study aimed at investigating the usefulness of FISH, as a diagnostic ancillary aid, to detect cytogenetic abnormalities such as MDM2 (murine double minute 2) amplification and CHOP(C/EBP homologous protein) rearrangement in liposarcoma, as well as SYT (synaptotagmin) rearrangement in synovial sarcoma. Methods: The FISH technique was used to analyze 17 specimens of liposarcoma for MDM2 amplification and CHOP rearrangement, and 10 specimens of synovial sarcoma for SYT rearrangement. The subtypes of liposarcoma and synovial sarcomas were reclassified according to the FISH results and compared with those of the respective histological findings. Results: According to the FISH results in 17 liposarcoma cases, well-differentiated liposarcoma(WDLPS), dedifferentiated liposarcoma (DDLPS), and myxoidliposarcoma (MLPS)subtypes were 41%, 53%, and 6%, respectively. In different subtypes of liposarcoma, a total of 30% mismatches were observed between pathologic and cytogenetic results. According to the histological findings from FISH analysis, SYT rearrangement was found only in three out of 10 (30%) synovial sarcomas. Conclusion: The detection of cytogenetic abnormalities in patients with liposarcoma and synovial sarcoma by FISH technique provides an important objective tool to confirm sarcoma diagnosis and sub classification of specific sarcoma subtypes in such patients.https://ijp.iranpath.org/article_25043_ac79674409a98de9ac72f09068eb05fa.pdfliposarcomasynovial sarcomafishchopsytmdm2 |
| spellingShingle | Farhad Shahi Razieh Alishahi Hossein Pashaiefar Isa Jahanzad Naser Kamalian Ardeshir Ghavamzadeh Marjan Yaghmaie Differentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ Hybridization Iranian Journal of Pathology liposarcoma synovial sarcoma fish chop syt mdm2 |
| title | Differentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ Hybridization |
| title_full | Differentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ Hybridization |
| title_fullStr | Differentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ Hybridization |
| title_full_unstemmed | Differentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ Hybridization |
| title_short | Differentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ Hybridization |
| title_sort | differentiating and categorizing of liposarcoma and synovial sarcoma neoplasms by fluorescence in situ hybridization |
| topic | liposarcoma synovial sarcoma fish chop syt mdm2 |
| url | https://ijp.iranpath.org/article_25043_ac79674409a98de9ac72f09068eb05fa.pdf |
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