Genome-Wide Identification of NAC Family Genes in Oat and Functional Characterization of <i>AsNAC109</i> in Abiotic Stress Tolerance
The plant-specific <i>NAC</i> gene family is one of the largest transcription factor families, participating in plant growth regulation and stress response. Despite extensive characterization in various plants, our knowledge of the <i>NAC</i> family in oat is lacking. Herein,...
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MDPI AG
2024-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/7/1017 |
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| author | Yahui Xu Jialong Cheng Haibin Hu Lin Yan Juqing Jia Bin Wu |
| author_facet | Yahui Xu Jialong Cheng Haibin Hu Lin Yan Juqing Jia Bin Wu |
| author_sort | Yahui Xu |
| collection | DOAJ |
| description | The plant-specific <i>NAC</i> gene family is one of the largest transcription factor families, participating in plant growth regulation and stress response. Despite extensive characterization in various plants, our knowledge of the <i>NAC</i> family in oat is lacking. Herein, we identified 333 <i>NAC</i> genes from the latest release of the common oat genome. We provide a comprehensive overview of the oat <i>NAC</i> gene family, covering gene structure, chromosomal localization, phylogenetic characteristics, conserved motif compositions, and gene duplications. <i>AsNAC</i> gene expression in different tissues and the response to various abiotic stresses were characterized using RT-qPCR. The main driver of oat <i>NAC</i> gene family expansion was identified as segmental duplication using collinearity analysis. In addition, the functions of <i>AsNAC109</i> in regulating abiotic stress tolerance in <i>Arabidopsis</i> were clarified. This is the first genome-wide investigation of the <i>NAC</i> gene family in cultivated oat, which provided a unique resource for subsequent research to elucidate the mechanisms responsible for oat stress tolerance and provides valuable clues for the improvement of stress resistance in cultivated oat. |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T10:36:24Z |
| publishDate | 2024-04-01 |
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| spelling | doaj.art-11d74a621b0149518c04adc366ec73092024-04-12T13:24:52ZengMDPI AGPlants2223-77472024-04-01137101710.3390/plants13071017Genome-Wide Identification of NAC Family Genes in Oat and Functional Characterization of <i>AsNAC109</i> in Abiotic Stress ToleranceYahui Xu0Jialong Cheng1Haibin Hu2Lin Yan3Juqing Jia4Bin Wu5College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Agriculture, Shanxi Agricultural University, Jinzhong 030801, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe plant-specific <i>NAC</i> gene family is one of the largest transcription factor families, participating in plant growth regulation and stress response. Despite extensive characterization in various plants, our knowledge of the <i>NAC</i> family in oat is lacking. Herein, we identified 333 <i>NAC</i> genes from the latest release of the common oat genome. We provide a comprehensive overview of the oat <i>NAC</i> gene family, covering gene structure, chromosomal localization, phylogenetic characteristics, conserved motif compositions, and gene duplications. <i>AsNAC</i> gene expression in different tissues and the response to various abiotic stresses were characterized using RT-qPCR. The main driver of oat <i>NAC</i> gene family expansion was identified as segmental duplication using collinearity analysis. In addition, the functions of <i>AsNAC109</i> in regulating abiotic stress tolerance in <i>Arabidopsis</i> were clarified. This is the first genome-wide investigation of the <i>NAC</i> gene family in cultivated oat, which provided a unique resource for subsequent research to elucidate the mechanisms responsible for oat stress tolerance and provides valuable clues for the improvement of stress resistance in cultivated oat.https://www.mdpi.com/2223-7747/13/7/1017oat<i>NAC</i> gene familygene duplicationexpression patternsabiotic stress |
| spellingShingle | Yahui Xu Jialong Cheng Haibin Hu Lin Yan Juqing Jia Bin Wu Genome-Wide Identification of NAC Family Genes in Oat and Functional Characterization of <i>AsNAC109</i> in Abiotic Stress Tolerance Plants oat <i>NAC</i> gene family gene duplication expression patterns abiotic stress |
| title | Genome-Wide Identification of NAC Family Genes in Oat and Functional Characterization of <i>AsNAC109</i> in Abiotic Stress Tolerance |
| title_full | Genome-Wide Identification of NAC Family Genes in Oat and Functional Characterization of <i>AsNAC109</i> in Abiotic Stress Tolerance |
| title_fullStr | Genome-Wide Identification of NAC Family Genes in Oat and Functional Characterization of <i>AsNAC109</i> in Abiotic Stress Tolerance |
| title_full_unstemmed | Genome-Wide Identification of NAC Family Genes in Oat and Functional Characterization of <i>AsNAC109</i> in Abiotic Stress Tolerance |
| title_short | Genome-Wide Identification of NAC Family Genes in Oat and Functional Characterization of <i>AsNAC109</i> in Abiotic Stress Tolerance |
| title_sort | genome wide identification of nac family genes in oat and functional characterization of i asnac109 i in abiotic stress tolerance |
| topic | oat <i>NAC</i> gene family gene duplication expression patterns abiotic stress |
| url | https://www.mdpi.com/2223-7747/13/7/1017 |
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