Short RNA guides cleavage by eukaryotic RNase III.
In eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution. However, the capacity of modern proteins to adopt new RNA guides has never been d...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2007-05-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0000472 |
| _version_ | 1818838063797764096 |
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| author | Bruno Lamontagne Sherif Abou Elela |
| author_facet | Bruno Lamontagne Sherif Abou Elela |
| author_sort | Bruno Lamontagne |
| collection | DOAJ |
| description | In eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution. However, the capacity of modern proteins to adopt new RNA guides has never been demonstrated. Here we show that Rnt1p, the yeast orthologue of the bacterial dsRNA-specific RNase III, can bind short RNA transcripts and use them as guides for sequence-specific cleavage. Target cleavage occurred at a constant distance from the Rnt1p binding site, leaving the guide RNA intact for subsequent cleavage. Our results indicate that RNase III may trigger sequence-specific RNA degradation independent of the RNAi machinery, and they open the road for a new generation of precise RNA silencing tools that do not trigger a dsRNA-mediated immune response. |
| first_indexed | 2024-12-19T03:32:27Z |
| format | Article |
| id | doaj.art-11db214113af4f6097d29ad3ba31c852 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T03:32:27Z |
| publishDate | 2007-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-11db214113af4f6097d29ad3ba31c8522022-12-21T20:37:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-05-0125e47210.1371/journal.pone.0000472Short RNA guides cleavage by eukaryotic RNase III.Bruno LamontagneSherif Abou ElelaIn eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution. However, the capacity of modern proteins to adopt new RNA guides has never been demonstrated. Here we show that Rnt1p, the yeast orthologue of the bacterial dsRNA-specific RNase III, can bind short RNA transcripts and use them as guides for sequence-specific cleavage. Target cleavage occurred at a constant distance from the Rnt1p binding site, leaving the guide RNA intact for subsequent cleavage. Our results indicate that RNase III may trigger sequence-specific RNA degradation independent of the RNAi machinery, and they open the road for a new generation of precise RNA silencing tools that do not trigger a dsRNA-mediated immune response.https://doi.org/10.1371/journal.pone.0000472 |
| spellingShingle | Bruno Lamontagne Sherif Abou Elela Short RNA guides cleavage by eukaryotic RNase III. PLoS ONE |
| title | Short RNA guides cleavage by eukaryotic RNase III. |
| title_full | Short RNA guides cleavage by eukaryotic RNase III. |
| title_fullStr | Short RNA guides cleavage by eukaryotic RNase III. |
| title_full_unstemmed | Short RNA guides cleavage by eukaryotic RNase III. |
| title_short | Short RNA guides cleavage by eukaryotic RNase III. |
| title_sort | short rna guides cleavage by eukaryotic rnase iii |
| url | https://doi.org/10.1371/journal.pone.0000472 |
| work_keys_str_mv | AT brunolamontagne shortrnaguidescleavagebyeukaryoticrnaseiii AT sherifabouelela shortrnaguidescleavagebyeukaryoticrnaseiii |