Comparative phylogenetic analysis of Dobrava-Belgrade virus L and S genetic segments isolated from an animal reservoir in Serbia
The Dobrava-Belgrade virus (DOBV) is a member of the Bunyaviridae family, genus Hantavirus, possessing a single-stranded RNA genome consisting of three segments, designated L (large), M (medium) and S (small). In this study, we present phylogenetic analysis of a newly detected DOBV strain i...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2014-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641402497N.pdf |
| _version_ | 1811242478362165248 |
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| author | Nikolić Valentina Stajković N. Stamenković Gorana Cekanac R. Marušić P. Jovanović N. Krstić Milena Mladenović J. Šiljić Marina Gligić Ana Stanojević Maja |
| author_facet | Nikolić Valentina Stajković N. Stamenković Gorana Cekanac R. Marušić P. Jovanović N. Krstić Milena Mladenović J. Šiljić Marina Gligić Ana Stanojević Maja |
| author_sort | Nikolić Valentina |
| collection | DOAJ |
| description | The Dobrava-Belgrade virus (DOBV) is a member of the Bunyaviridae family,
genus Hantavirus, possessing a single-stranded RNA genome consisting of three
segments, designated L (large), M (medium) and S (small). In this study, we
present phylogenetic analysis of a newly detected DOBV strain isolated from
Apodemus agrarius. Analysis was based on partial L and S segment sequences,
in comparison to previously published DOBV sequences from Serbia and
elsewhere. A phylogenetic tree based on partial S segment revealed local
geographical clustering of DOBV sequences from Serbia, unrelated to host
(rodent or human). The topology of the phylogenetic tree was confirmed with a
high percent of completely or partially resolved quartets in
likelihood-mapping analysis, whereas no evidence of possible recombination in
the examined S segment data set was found. |
| first_indexed | 2024-04-12T13:51:50Z |
| format | Article |
| id | doaj.art-11df4c644e88485bb7f2331a0cd8f037 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-12T13:51:50Z |
| publishDate | 2014-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-11df4c644e88485bb7f2331a0cd8f0372022-12-22T03:30:29ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392014-01-0166249750610.2298/ABS1402497N0354-46641402497NComparative phylogenetic analysis of Dobrava-Belgrade virus L and S genetic segments isolated from an animal reservoir in SerbiaNikolić Valentina0Stajković N.1Stamenković Gorana2Cekanac R.3Marušić P.4Jovanović N.5Krstić Milena6Mladenović J.7Šiljić Marina8Gligić Ana9Stanojević Maja10Faculty of Medicine, BelgradeMilitary Medical Academy, BelgradeInstitute for Biological Research “Siniša Stanković”, BelgradeMilitary Medical Academy, BelgradeInstitute of Public Health, ZaječarMinistry of Defense, BelgradeMilitary Medical Academy, BelgradeMilitary Medical Academy, BelgradeFaculty of Medicine, BelgradeInstitute of Virology, Vaccines and Sera Torlak, BelgradeFaculty of Medicine, BelgradeThe Dobrava-Belgrade virus (DOBV) is a member of the Bunyaviridae family, genus Hantavirus, possessing a single-stranded RNA genome consisting of three segments, designated L (large), M (medium) and S (small). In this study, we present phylogenetic analysis of a newly detected DOBV strain isolated from Apodemus agrarius. Analysis was based on partial L and S segment sequences, in comparison to previously published DOBV sequences from Serbia and elsewhere. A phylogenetic tree based on partial S segment revealed local geographical clustering of DOBV sequences from Serbia, unrelated to host (rodent or human). The topology of the phylogenetic tree was confirmed with a high percent of completely or partially resolved quartets in likelihood-mapping analysis, whereas no evidence of possible recombination in the examined S segment data set was found.http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641402497N.pdfDOBVphylogenetic analysislikelihood mappingrecombination |
| spellingShingle | Nikolić Valentina Stajković N. Stamenković Gorana Cekanac R. Marušić P. Jovanović N. Krstić Milena Mladenović J. Šiljić Marina Gligić Ana Stanojević Maja Comparative phylogenetic analysis of Dobrava-Belgrade virus L and S genetic segments isolated from an animal reservoir in Serbia Archives of Biological Sciences DOBV phylogenetic analysis likelihood mapping recombination |
| title | Comparative phylogenetic analysis of Dobrava-Belgrade virus L and S genetic segments isolated from an animal reservoir in Serbia |
| title_full | Comparative phylogenetic analysis of Dobrava-Belgrade virus L and S genetic segments isolated from an animal reservoir in Serbia |
| title_fullStr | Comparative phylogenetic analysis of Dobrava-Belgrade virus L and S genetic segments isolated from an animal reservoir in Serbia |
| title_full_unstemmed | Comparative phylogenetic analysis of Dobrava-Belgrade virus L and S genetic segments isolated from an animal reservoir in Serbia |
| title_short | Comparative phylogenetic analysis of Dobrava-Belgrade virus L and S genetic segments isolated from an animal reservoir in Serbia |
| title_sort | comparative phylogenetic analysis of dobrava belgrade virus l and s genetic segments isolated from an animal reservoir in serbia |
| topic | DOBV phylogenetic analysis likelihood mapping recombination |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641402497N.pdf |
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