HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution
Using Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All w...
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/8/2667 |
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| author | Katarzyna Sitarz Krzysztof Czamara Joanna Bialecka Malgorzata Klimek Barbara Zawilinska Slawa Szostek Agnieszka Kaczor |
| author_facet | Katarzyna Sitarz Krzysztof Czamara Joanna Bialecka Malgorzata Klimek Barbara Zawilinska Slawa Szostek Agnieszka Kaczor |
| author_sort | Katarzyna Sitarz |
| collection | DOAJ |
| description | Using Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All women were tested for human papillomavirus (HPV) infection using PCR. Subcellular resolution of Raman microscopy enabled to understand phenotypic differences in a heterogeneous population of cervical cells in the following groups: I/HPV<sup>−</sup>, IIa/HPV<sup>−</sup>, IIa/HPV<sup>−</sup>, LSIL/HPV<sup>−</sup>, LSIL/HPV<sup>+</sup>, HSIL/HPV<sup>−</sup>, HSIL/HPV<sup>+</sup> and cancer cells (SCC/HPV<sup>+</sup>). We showed for the first time that the glycogen content in the cytoplasm decreased with the nucleus size of cervical cells in all studied groups apart from the cancer group. For the subpopulation of large-nucleus cells HPV infection resulted in considerable glycogen depletion compared to HPV negative cells in IIa, LSIL (for both statistical significance, ca. 45%) and HSIL (trend, 37%) groups. We hypothesize that accelerated glycogenolysis in large-nucleus cells may be associated with the increased protein metabolism for HPV positive cells. Our work underlines unique capabilities of Raman microscopy in single cell studies and demonstrate potential of Raman-based methods in HPV diagnostics. |
| first_indexed | 2024-03-10T20:31:38Z |
| format | Article |
| id | doaj.art-11df679c7ebe4501b0d6bbb74bdee0b0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:31:38Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-11df679c7ebe4501b0d6bbb74bdee0b02023-11-19T21:21:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01218266710.3390/ijms21082667HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular ResolutionKatarzyna Sitarz0Krzysztof Czamara1Joanna Bialecka2Malgorzata Klimek3Barbara Zawilinska4Slawa Szostek5Agnieszka Kaczor6Department of Virology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Krakow, PolandJagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, 14 Bobrzynskiego Street, 30-348 Krakow, PolandCentre of Microbiological Research and Autovaccines, 17 Slawkowska Street, 31-016 Krakow, PolandNational Research Institute of Oncology, Krakow Branch, Clinic of Radiotherapy, 11 Garncarska Street, 31-115 Krakow, PolandDepartment of Virology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Krakow, PolandDepartment of Virology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Krakow, PolandFaculty of Chemistry, Jagiellonian University, 2 Gronostajowa Street, 30-387 Krakow, PolandUsing Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All women were tested for human papillomavirus (HPV) infection using PCR. Subcellular resolution of Raman microscopy enabled to understand phenotypic differences in a heterogeneous population of cervical cells in the following groups: I/HPV<sup>−</sup>, IIa/HPV<sup>−</sup>, IIa/HPV<sup>−</sup>, LSIL/HPV<sup>−</sup>, LSIL/HPV<sup>+</sup>, HSIL/HPV<sup>−</sup>, HSIL/HPV<sup>+</sup> and cancer cells (SCC/HPV<sup>+</sup>). We showed for the first time that the glycogen content in the cytoplasm decreased with the nucleus size of cervical cells in all studied groups apart from the cancer group. For the subpopulation of large-nucleus cells HPV infection resulted in considerable glycogen depletion compared to HPV negative cells in IIa, LSIL (for both statistical significance, ca. 45%) and HSIL (trend, 37%) groups. We hypothesize that accelerated glycogenolysis in large-nucleus cells may be associated with the increased protein metabolism for HPV positive cells. Our work underlines unique capabilities of Raman microscopy in single cell studies and demonstrate potential of Raman-based methods in HPV diagnostics.https://www.mdpi.com/1422-0067/21/8/2667human papillomavirusglycogencervical cancercervical dysplasiaRaman microscopyglycogenolysis |
| spellingShingle | Katarzyna Sitarz Krzysztof Czamara Joanna Bialecka Malgorzata Klimek Barbara Zawilinska Slawa Szostek Agnieszka Kaczor HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution International Journal of Molecular Sciences human papillomavirus glycogen cervical cancer cervical dysplasia Raman microscopy glycogenolysis |
| title | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
| title_full | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
| title_fullStr | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
| title_full_unstemmed | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
| title_short | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
| title_sort | hpv infection significantly accelerates glycogen metabolism in cervical cells with large nuclei raman microscopic study with subcellular resolution |
| topic | human papillomavirus glycogen cervical cancer cervical dysplasia Raman microscopy glycogenolysis |
| url | https://www.mdpi.com/1422-0067/21/8/2667 |
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