Transcript-Level Dysregulation of <i>BCL2</i> Family Genes in Acute Myeloblastic Leukemia
The expression of apoptosis-related <i>BCL2</i> family genes, fine-tuned in normal cells, is dysregulated in many neoplasms. In acute myeloid leukemia (AML), this problem has not been studied comprehensively. To address this issue, RNA-seq data were used to analyze the expression of 26 B...
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MDPI AG
2021-06-01
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| author | Luiza Handschuh Pawel Wojciechowski Maciej Kazmierczak Krzysztof Lewandowski |
| author_facet | Luiza Handschuh Pawel Wojciechowski Maciej Kazmierczak Krzysztof Lewandowski |
| author_sort | Luiza Handschuh |
| collection | DOAJ |
| description | The expression of apoptosis-related <i>BCL2</i> family genes, fine-tuned in normal cells, is dysregulated in many neoplasms. In acute myeloid leukemia (AML), this problem has not been studied comprehensively. To address this issue, RNA-seq data were used to analyze the expression of 26 BCL2 family members in 27 AML FAB M1 and M2 patients, divided into subgroups differently responding to chemotherapy. A correlation analysis, analysis of variance, and Kaplan-Meier analysis were applied to associate the expression of particular genes with other gene expression, clinical features, and the presence of mutations detected by exome sequencing. The expression of <i>BCL2</i> family genes was dysregulated in AML, as compared to healthy controls. An upregulation of anti-apoptotic and downregulation of pro-apoptotic genes was observed, though only a decrease in <i>BMF</i>, <i>BNIP1</i>, and <i>HRK</i> was statistically significant. In a group of patients resistant to chemotherapy, overexpression of <i>BCL2L1</i> was manifested. In agreement with the literature data, our results reveal that <i>BCL2L1</i> is one of the key players in apoptosis regulation in different types of tumors. An exome sequencing data analysis indicates that <i>BCL2</i> family genes are not mutated in AML, but their expression is correlated with the mutational status of other genes, including those recurrently mutated in AML and splicing-related. High levels of some <i>BCL2</i> family members, in particular <i>BIK</i> and <i>BCL2L13</i>, were associated with poor outcome. |
| first_indexed | 2024-03-10T10:03:16Z |
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| last_indexed | 2024-03-10T10:03:16Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
| series | Cancers |
| spelling | doaj.art-11e3246d0b70446ca6814babd7a5aeaf2023-11-22T01:45:02ZengMDPI AGCancers2072-66942021-06-011313317510.3390/cancers13133175Transcript-Level Dysregulation of <i>BCL2</i> Family Genes in Acute Myeloblastic LeukemiaLuiza Handschuh0Pawel Wojciechowski1Maciej Kazmierczak2Krzysztof Lewandowski3Laboratory of Genomics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznan, PolandLaboratory of Genomics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznan, PolandDepartment of Hematology and Bone Marrow Transplantation, Poznan University of Medical Sciences, 60-569 Poznan, PolandDepartment of Hematology and Bone Marrow Transplantation, Poznan University of Medical Sciences, 60-569 Poznan, PolandThe expression of apoptosis-related <i>BCL2</i> family genes, fine-tuned in normal cells, is dysregulated in many neoplasms. In acute myeloid leukemia (AML), this problem has not been studied comprehensively. To address this issue, RNA-seq data were used to analyze the expression of 26 BCL2 family members in 27 AML FAB M1 and M2 patients, divided into subgroups differently responding to chemotherapy. A correlation analysis, analysis of variance, and Kaplan-Meier analysis were applied to associate the expression of particular genes with other gene expression, clinical features, and the presence of mutations detected by exome sequencing. The expression of <i>BCL2</i> family genes was dysregulated in AML, as compared to healthy controls. An upregulation of anti-apoptotic and downregulation of pro-apoptotic genes was observed, though only a decrease in <i>BMF</i>, <i>BNIP1</i>, and <i>HRK</i> was statistically significant. In a group of patients resistant to chemotherapy, overexpression of <i>BCL2L1</i> was manifested. In agreement with the literature data, our results reveal that <i>BCL2L1</i> is one of the key players in apoptosis regulation in different types of tumors. An exome sequencing data analysis indicates that <i>BCL2</i> family genes are not mutated in AML, but their expression is correlated with the mutational status of other genes, including those recurrently mutated in AML and splicing-related. High levels of some <i>BCL2</i> family members, in particular <i>BIK</i> and <i>BCL2L13</i>, were associated with poor outcome.https://www.mdpi.com/2072-6694/13/13/3175AML<i>BCL2</i> familyapoptosisgene expressionRNA-seqexome sequencing |
| spellingShingle | Luiza Handschuh Pawel Wojciechowski Maciej Kazmierczak Krzysztof Lewandowski Transcript-Level Dysregulation of <i>BCL2</i> Family Genes in Acute Myeloblastic Leukemia Cancers AML <i>BCL2</i> family apoptosis gene expression RNA-seq exome sequencing |
| title | Transcript-Level Dysregulation of <i>BCL2</i> Family Genes in Acute Myeloblastic Leukemia |
| title_full | Transcript-Level Dysregulation of <i>BCL2</i> Family Genes in Acute Myeloblastic Leukemia |
| title_fullStr | Transcript-Level Dysregulation of <i>BCL2</i> Family Genes in Acute Myeloblastic Leukemia |
| title_full_unstemmed | Transcript-Level Dysregulation of <i>BCL2</i> Family Genes in Acute Myeloblastic Leukemia |
| title_short | Transcript-Level Dysregulation of <i>BCL2</i> Family Genes in Acute Myeloblastic Leukemia |
| title_sort | transcript level dysregulation of i bcl2 i family genes in acute myeloblastic leukemia |
| topic | AML <i>BCL2</i> family apoptosis gene expression RNA-seq exome sequencing |
| url | https://www.mdpi.com/2072-6694/13/13/3175 |
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