Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction

Objective: The aim of this study was to evaluate the percentage of pregnant women with negative Group B Streptococcus (GBS) screening results by culture at 35 weeks' gestation, who subsequently had positive GBS test results after 39 weeks' gestation. Materials and Methods: From 2006 to 200...

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Main Authors: Chi-Feng Wei, Bo-Ching She, Hung-Shuo Liang, Qing-Dong Ling, Chen-Yuan Tsai, Chih-Wei Yen, Hsueh-Yin Wu, Ming-Song Tsai
Format: Article
Language:English
Published: Elsevier 2009-06-01
Series:Taiwanese Journal of Obstetrics & Gynecology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1028455909602692
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author Chi-Feng Wei
Bo-Ching She
Hung-Shuo Liang
Qing-Dong Ling
Chen-Yuan Tsai
Chih-Wei Yen
Hsueh-Yin Wu
Ming-Song Tsai
author_facet Chi-Feng Wei
Bo-Ching She
Hung-Shuo Liang
Qing-Dong Ling
Chen-Yuan Tsai
Chih-Wei Yen
Hsueh-Yin Wu
Ming-Song Tsai
author_sort Chi-Feng Wei
collection DOAJ
description Objective: The aim of this study was to evaluate the percentage of pregnant women with negative Group B Streptococcus (GBS) screening results by culture at 35 weeks' gestation, who subsequently had positive GBS test results after 39 weeks' gestation. Materials and Methods: From 2006 to 2007, we recruited 150 pregnant women who received routine GBS culture screening at 35 weeks' gestation with negative results, and who had repeat cultures and real-time polymerase chain reaction (RT-PCR) tests for GBS after 39 weeks' gestation. Results: Two percent of pregnant women with GBS-negative results by culture screening at 35 weeks' gestation were GBS-positive at 39 weeks' gestation. Conclusion: It is necessary to perform a GBS test 4 weeks after an initial negative GBS culture at 35–37 weeks of gestation. RT-PCR provides a simple and rapid alternative method for detecting rectovaginal GBS colonization at the time of labor.
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spelling doaj.art-11e729f3a7ef45e29a4d9869ba3208f42022-12-21T19:12:00ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592009-06-0148211611910.1016/S1028-4559(09)60269-2Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain ReactionChi-Feng Wei0Bo-Ching She1Hung-Shuo Liang2Qing-Dong Ling3Chen-Yuan Tsai4Chih-Wei Yen5Hsueh-Yin Wu6Ming-Song Tsai7Department of Obstetrics and Gynecology, Cathay General Hospital, Si-Jhih, TaiwanDepartment of Obstetrics and Gynecology, Cathay General Hospital, Si-Jhih, TaiwanDepartment of Obstetrics and Gynecology, Cathay General Hospital, Si-Jhih, TaiwanCathay Medical Research Institute, Taipei, TaiwanDepartment of Laboratory Medicine, Cathay General Hospital, Si-Jhih, TaiwanDepartment of Pediatrics, Cathay General Hospital, Si-Jhih, TaiwanDepartment of Laboratory Medicine, Cathay General Hospital, Si-Jhih, TaiwanDepartment of Obstetrics and Gynecology, Cathay General Hospital, Taipei, TaiwanObjective: The aim of this study was to evaluate the percentage of pregnant women with negative Group B Streptococcus (GBS) screening results by culture at 35 weeks' gestation, who subsequently had positive GBS test results after 39 weeks' gestation. Materials and Methods: From 2006 to 2007, we recruited 150 pregnant women who received routine GBS culture screening at 35 weeks' gestation with negative results, and who had repeat cultures and real-time polymerase chain reaction (RT-PCR) tests for GBS after 39 weeks' gestation. Results: Two percent of pregnant women with GBS-negative results by culture screening at 35 weeks' gestation were GBS-positive at 39 weeks' gestation. Conclusion: It is necessary to perform a GBS test 4 weeks after an initial negative GBS culture at 35–37 weeks of gestation. RT-PCR provides a simple and rapid alternative method for detecting rectovaginal GBS colonization at the time of labor.http://www.sciencedirect.com/science/article/pii/S1028455909602692group B streptococcimaternal screeningpregnant womenreal-time polymerase chain reaction
spellingShingle Chi-Feng Wei
Bo-Ching She
Hung-Shuo Liang
Qing-Dong Ling
Chen-Yuan Tsai
Chih-Wei Yen
Hsueh-Yin Wu
Ming-Song Tsai
Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction
Taiwanese Journal of Obstetrics & Gynecology
group B streptococci
maternal screening
pregnant women
real-time polymerase chain reaction
title Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction
title_full Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction
title_fullStr Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction
title_full_unstemmed Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction
title_short Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction
title_sort prenatal group b streptococcus test using real time polymerase chain reaction
topic group B streptococci
maternal screening
pregnant women
real-time polymerase chain reaction
url http://www.sciencedirect.com/science/article/pii/S1028455909602692
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AT hungshuoliang prenatalgroupbstreptococcustestusingrealtimepolymerasechainreaction
AT qingdongling prenatalgroupbstreptococcustestusingrealtimepolymerasechainreaction
AT chenyuantsai prenatalgroupbstreptococcustestusingrealtimepolymerasechainreaction
AT chihweiyen prenatalgroupbstreptococcustestusingrealtimepolymerasechainreaction
AT hsuehyinwu prenatalgroupbstreptococcustestusingrealtimepolymerasechainreaction
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