| Summary: | Characterizing the complete mitochondrial genome (mitogenome) of an organism is useful for genomic studies in taxonomy and evolution. The mitogenomic characteristics of <i>Eucrate crenata</i> (Decapoda: Brachyura: Goneplacidae) have never been studied. The present study decodes the first mitogenome of <i>E. crenata</i> by high-throughput sequencing (HTS). The length of the mitogenome is 15,597 bp, and it contains 13 protein-coding genes, 2 ribosomal RNA genes (<i>rrnS</i> and <i>rrnL</i>), and 22 transfer RNA genes. There are 14 and 23 genes observed on the heavy and light strands, respectively. <i>E. crenata</i> possesses a <i>trnH-cac</i> translocation, with the <i>trnH-cac</i> shifted between <i>trnE-gaa</i> and <i>trnF-ttc</i> instead of the usual location between <i>nad5</i> and <i>nad4</i> in decapods. Phylogenetic analyses based on the current dataset of 33 Brachyuran mitogenomes indicate that <i>E. crenata</i>. is closely related to <i>Ashtoret lunaris</i> of Matutidae. The similar codon usage and rearrangements in the two species provide evidence for their close phylogenetic relationship. Positive selection analysis showed that one residue located in <i>cox1</i> was identified as a positively selected site with high BEB value (>95%), indicating that this gene was under positive selection pressure. This study is the first complete mitogenome record for the family Goneplacidae, and the results obtained may improve the understanding of the phylogeny of Goneplacidae in Brachyura.
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