Generation of Immune Inhibitory Dendritic Cells and
Variety of positive as well as negative regulatory signals are provided by antigen presenting cell in particular by dendritic cells. In this research, we studied the capacity of dendritic cells to expand antigen-specific T regulatory cells.We also investigated the role of TGF-beta in induction inhib...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2009-03-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/230 |
| _version_ | 1818026430730076160 |
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| author | Abediankenari Saeid Ghasemi Maryam |
| author_facet | Abediankenari Saeid Ghasemi Maryam |
| author_sort | Abediankenari Saeid |
| collection | DOAJ |
| description | Variety of positive as well as negative regulatory signals are provided by antigen presenting cell in particular by dendritic cells. In this research, we studied the capacity of dendritic cells to expand antigen-specific T regulatory cells.We also investigated the role of TGF-beta in induction inhibitory functions of dendritic cells in mixed leukocyte reactions.
Dendritic cells were generated from blood CD14+ monocytes with granulocyte-Monocyte colony stimulating factor and interleukin-4 with or without TGF-beta (TGF-β-GM-DC or GM-DC). CD4+ T cell were isolated to assess lymphocyte proliferation by lymphocyte transformation test assay and the ratio of CD4+FOXp3+ CD25+ T cells were determined by fluorescene-activated cell sorter.
T cell proliferation responses in GM-DC showed a significance antigen-specific proliferative response comparing with TGFβ-GM -DC. T Cell proliferation was inhibited in co-culture system containing DC-treated TGF-β.
It can be suggested that the expsansion of T regulatory by TGF-β-GM-DC provides a means for antigen specific control of unwanted immune reactions. |
| first_indexed | 2024-12-10T04:31:53Z |
| format | Article |
| id | doaj.art-11e7a0a226c6462fb87b528dd28be51c |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-10T04:31:53Z |
| publishDate | 2009-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-11e7a0a226c6462fb87b528dd28be51c2022-12-22T02:02:07ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492009-03-0181230Generation of Immune Inhibitory Dendritic Cells andAbediankenari Saeid0Ghasemi Maryam1Department of Microbiology and Immunology, School of Medicine, 2 Department of Pathology, School of Medicine, Mazandaran University of Medical Sciences, Sari, IranVariety of positive as well as negative regulatory signals are provided by antigen presenting cell in particular by dendritic cells. In this research, we studied the capacity of dendritic cells to expand antigen-specific T regulatory cells.We also investigated the role of TGF-beta in induction inhibitory functions of dendritic cells in mixed leukocyte reactions. Dendritic cells were generated from blood CD14+ monocytes with granulocyte-Monocyte colony stimulating factor and interleukin-4 with or without TGF-beta (TGF-β-GM-DC or GM-DC). CD4+ T cell were isolated to assess lymphocyte proliferation by lymphocyte transformation test assay and the ratio of CD4+FOXp3+ CD25+ T cells were determined by fluorescene-activated cell sorter. T cell proliferation responses in GM-DC showed a significance antigen-specific proliferative response comparing with TGFβ-GM -DC. T Cell proliferation was inhibited in co-culture system containing DC-treated TGF-β. It can be suggested that the expsansion of T regulatory by TGF-β-GM-DC provides a means for antigen specific control of unwanted immune reactions.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/230 |
| spellingShingle | Abediankenari Saeid Ghasemi Maryam Generation of Immune Inhibitory Dendritic Cells and Iranian Journal of Allergy, Asthma and Immunology |
| title | Generation of Immune Inhibitory Dendritic Cells and |
| title_full | Generation of Immune Inhibitory Dendritic Cells and |
| title_fullStr | Generation of Immune Inhibitory Dendritic Cells and |
| title_full_unstemmed | Generation of Immune Inhibitory Dendritic Cells and |
| title_short | Generation of Immune Inhibitory Dendritic Cells and |
| title_sort | generation of immune inhibitory dendritic cells and |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/230 |
| work_keys_str_mv | AT abediankenarisaeid generationofimmuneinhibitorydendriticcellsand AT ghasemimaryam generationofimmuneinhibitorydendriticcellsand |