Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma (DLBCL)
Purpose: Given the importance of understanding the genetic variations involved in the pathogenesis of non-Hodgkin’s lymphoma (NHL), this work was designed to study the impact of IL-10 (−1082 G/A; rs1800896 and −819 C/T; rs1800871) gene promoter polymorphism on susceptibility of Egyptians to diffuse...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2014-01-01
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| Series: | Egyptian Journal of Medical Human Genetics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110863013000633 |
| _version_ | 1819179580335849472 |
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| author | Roba M. Talaat Amal M. Abdel-Aziz Eman A. El-Maadawy Naser Abdel-Bary |
| author_facet | Roba M. Talaat Amal M. Abdel-Aziz Eman A. El-Maadawy Naser Abdel-Bary |
| author_sort | Roba M. Talaat |
| collection | DOAJ |
| description | Purpose: Given the importance of understanding the genetic variations involved in the pathogenesis of non-Hodgkin’s lymphoma (NHL), this work was designed to study the impact of IL-10 (−1082 G/A; rs1800896 and −819 C/T; rs1800871) gene promoter polymorphism on susceptibility of Egyptians to diffuse large B cell lymphoma (DLBCL); the major type of NHL. To the best of our knowledge, this study is the first one that examines IL-10 promoter polymorphism in DLBCL in Egyptians.
Methods: Genotyping polymorphism is performed using sequence-specific primers polymerase chain reaction (SSP-PCR) in 100 Egyptian DLBCL patients and 119 normal controls. Circulating plasma levels of IL-10 were measured using Enzyme-linked immunosorbent assay (ELISA).
Results: Insignificant change in IL-10 (−1082 and −819) genotypes was recorded. Although A allele is slightly decreased in DLBCL patients, it did not reach statistical significance. GT haplotype was significantly elevated (P < 0.05) in NHL patients. A significant linkage disequilibrium between the −1082 and −819 SNPs with D′ = 0.596 and r2 = 0.1032 (P < 0.001) was demonstrated. Significantly increased plasma IL-10 (P < 0.01) was found which is positively correlated (r = 0.307; P < 0.01) with the disease.
Conclusions: Taken together, our findings demonstrated that IL-10 promoter gene polymorphism (−1082 and −819) may not have an influence on the clinical outcome of DLBCL, especially in terms of overall secretion level. Further investigations of other cytokine gene polymorphisms will lead to a better understanding of the disease’s biological background. |
| first_indexed | 2024-12-22T22:00:42Z |
| format | Article |
| id | doaj.art-11eb4796544447419610f8547ba34577 |
| institution | Directory Open Access Journal |
| issn | 1110-8630 |
| language | English |
| last_indexed | 2024-12-22T22:00:42Z |
| publishDate | 2014-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-11eb4796544447419610f8547ba345772022-12-21T18:11:08ZengSpringerOpenEgyptian Journal of Medical Human Genetics1110-86302014-01-0115171310.1016/j.ejmhg.2013.09.001Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma (DLBCL)Roba M. Talaat0Amal M. Abdel-Aziz1Eman A. El-Maadawy2Naser Abdel-Bary3Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Menufia University, Sadat Branch, EgyptMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Menufia University, Sadat Branch, EgyptMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Menufia University, Sadat Branch, EgyptClinical Oncology Department, Faculty of Medicine, Menufia University, EgyptPurpose: Given the importance of understanding the genetic variations involved in the pathogenesis of non-Hodgkin’s lymphoma (NHL), this work was designed to study the impact of IL-10 (−1082 G/A; rs1800896 and −819 C/T; rs1800871) gene promoter polymorphism on susceptibility of Egyptians to diffuse large B cell lymphoma (DLBCL); the major type of NHL. To the best of our knowledge, this study is the first one that examines IL-10 promoter polymorphism in DLBCL in Egyptians. Methods: Genotyping polymorphism is performed using sequence-specific primers polymerase chain reaction (SSP-PCR) in 100 Egyptian DLBCL patients and 119 normal controls. Circulating plasma levels of IL-10 were measured using Enzyme-linked immunosorbent assay (ELISA). Results: Insignificant change in IL-10 (−1082 and −819) genotypes was recorded. Although A allele is slightly decreased in DLBCL patients, it did not reach statistical significance. GT haplotype was significantly elevated (P < 0.05) in NHL patients. A significant linkage disequilibrium between the −1082 and −819 SNPs with D′ = 0.596 and r2 = 0.1032 (P < 0.001) was demonstrated. Significantly increased plasma IL-10 (P < 0.01) was found which is positively correlated (r = 0.307; P < 0.01) with the disease. Conclusions: Taken together, our findings demonstrated that IL-10 promoter gene polymorphism (−1082 and −819) may not have an influence on the clinical outcome of DLBCL, especially in terms of overall secretion level. Further investigations of other cytokine gene polymorphisms will lead to a better understanding of the disease’s biological background.http://www.sciencedirect.com/science/article/pii/S1110863013000633NHLDLBCLSNPIL-10ELISA |
| spellingShingle | Roba M. Talaat Amal M. Abdel-Aziz Eman A. El-Maadawy Naser Abdel-Bary Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma (DLBCL) Egyptian Journal of Medical Human Genetics NHL DLBCL SNP IL-10 ELISA |
| title | Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma (DLBCL) |
| title_full | Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma (DLBCL) |
| title_fullStr | Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma (DLBCL) |
| title_full_unstemmed | Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma (DLBCL) |
| title_short | Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma (DLBCL) |
| title_sort | interleukin 10 gene promoter polymorphism and risk of diffuse large b cell lymphoma dlbcl |
| topic | NHL DLBCL SNP IL-10 ELISA |
| url | http://www.sciencedirect.com/science/article/pii/S1110863013000633 |
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