Evaluation of the immunopathological response to bcg vaccine in a xenogeneic immunocompetent animal
Mycobacterium bovis, the causative agent of bovine tuberculosis (BTB), is one of the most significant endemic diseases confronting government, veterinary professionals, and farming industry worldwide nowadays. M. bovis has not only a negative impact on bovine health and economy, but also poses a thr...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2023-05-01
|
| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2998 |
| _version_ | 1827850767867838464 |
|---|---|
| author | Salma A. SHOULAH Mohamed M. GABALLA Said M. ELSHAFAE Maha A. MOUSSA Abdelfattah SELIM Majed H. WAKID |
| author_facet | Salma A. SHOULAH Mohamed M. GABALLA Said M. ELSHAFAE Maha A. MOUSSA Abdelfattah SELIM Majed H. WAKID |
| author_sort | Salma A. SHOULAH |
| collection | DOAJ |
| description | Mycobacterium bovis, the causative agent of bovine tuberculosis (BTB), is one of the most significant endemic diseases confronting
government, veterinary professionals, and farming industry worldwide nowadays. M. bovis has not only a negative impact on bovine health
and economy, but also poses a threat to public health as a zoonotic disease that could be transmitted from animal to human. Although,
bacillus Calmette-Guérin (BCG) vaccine of M. bovis has been extensively used in many animals, only few studies had reported its side
effects in these animals. In this study, the systemic pathological lesions and immunoglobulin levels associated with intranasal (IN) and
subcutaneous (SC) injection of BCG vaccine in Swiss male mice have been evaluated. The results revealed an elevation in IgM and IgA
levels in both routes (nebulization, subcutaneous injection) while there was a dramatic increase in IgG levels in subcutaneously injected
mice. Aerosolization of BCG vaccine using a nebulizer resulted in severe pulmonary lesions with numerous megakaryocytes in the spleens
of mice. On the other hand, SC injection had mild effect on pulmonary tissues and induced moderate extramedullary hematopoiesis in
the hepatic tissues of mice. In conclusion, inadvertent vaccination of BCG in Swiss mice, triggered adverse tissue reaction and remarkable
increase in Ig level. The severity of tissue lesions corresponded to the injection route in mice. |
| first_indexed | 2024-03-12T10:23:22Z |
| format | Article |
| id | doaj.art-11eb81782392415c91658a42833936bd |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-12T10:23:22Z |
| publishDate | 2023-05-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-11eb81782392415c91658a42833936bd2023-09-02T09:54:26ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-05-0129328128810.9775/kvfd.2023.291872998Evaluation of the immunopathological response to bcg vaccine in a xenogeneic immunocompetent animalSalma A. SHOULAH0Mohamed M. GABALLA1Said M. ELSHAFAE2Maha A. MOUSSA3Abdelfattah SELIM4Majed H. WAKID5Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Moshtohor, Tukh, Qalyobiya, 13736 EGYPTDepartment of Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Tukh, Qalyobiya, 13736 EGYPTDepartment of Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Tukh, Qalyobiya, 13736 EGYPTDepartment of Statistics, Faculty of Commerce, Benha University, Benha, Qalyobiya, EGYPTDepartment of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Moshtohor, Tukh, Qalyobiya, 13736 EGYPTDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, SAUDI ARABIAMycobacterium bovis, the causative agent of bovine tuberculosis (BTB), is one of the most significant endemic diseases confronting government, veterinary professionals, and farming industry worldwide nowadays. M. bovis has not only a negative impact on bovine health and economy, but also poses a threat to public health as a zoonotic disease that could be transmitted from animal to human. Although, bacillus Calmette-Guérin (BCG) vaccine of M. bovis has been extensively used in many animals, only few studies had reported its side effects in these animals. In this study, the systemic pathological lesions and immunoglobulin levels associated with intranasal (IN) and subcutaneous (SC) injection of BCG vaccine in Swiss male mice have been evaluated. The results revealed an elevation in IgM and IgA levels in both routes (nebulization, subcutaneous injection) while there was a dramatic increase in IgG levels in subcutaneously injected mice. Aerosolization of BCG vaccine using a nebulizer resulted in severe pulmonary lesions with numerous megakaryocytes in the spleens of mice. On the other hand, SC injection had mild effect on pulmonary tissues and induced moderate extramedullary hematopoiesis in the hepatic tissues of mice. In conclusion, inadvertent vaccination of BCG in Swiss mice, triggered adverse tissue reaction and remarkable increase in Ig level. The severity of tissue lesions corresponded to the injection route in mice.https://vetdergikafkas.org/pdf.php?id=2998aerosolbcgextramedullary hematopoiesismicevaccine |
| spellingShingle | Salma A. SHOULAH Mohamed M. GABALLA Said M. ELSHAFAE Maha A. MOUSSA Abdelfattah SELIM Majed H. WAKID Evaluation of the immunopathological response to bcg vaccine in a xenogeneic immunocompetent animal Kafkas Universitesi Veteriner Fakültesi Dergisi aerosol bcg extramedullary hematopoiesis mice vaccine |
| title | Evaluation of the immunopathological response to bcg vaccine in a xenogeneic immunocompetent animal |
| title_full | Evaluation of the immunopathological response to bcg vaccine in a xenogeneic immunocompetent animal |
| title_fullStr | Evaluation of the immunopathological response to bcg vaccine in a xenogeneic immunocompetent animal |
| title_full_unstemmed | Evaluation of the immunopathological response to bcg vaccine in a xenogeneic immunocompetent animal |
| title_short | Evaluation of the immunopathological response to bcg vaccine in a xenogeneic immunocompetent animal |
| title_sort | evaluation of the immunopathological response to bcg vaccine in a xenogeneic immunocompetent animal |
| topic | aerosol bcg extramedullary hematopoiesis mice vaccine |
| url | https://vetdergikafkas.org/pdf.php?id=2998 |
| work_keys_str_mv | AT salmaashoulah evaluationoftheimmunopathologicalresponsetobcgvaccineinaxenogeneicimmunocompetentanimal AT mohamedmgaballa evaluationoftheimmunopathologicalresponsetobcgvaccineinaxenogeneicimmunocompetentanimal AT saidmelshafae evaluationoftheimmunopathologicalresponsetobcgvaccineinaxenogeneicimmunocompetentanimal AT mahaamoussa evaluationoftheimmunopathologicalresponsetobcgvaccineinaxenogeneicimmunocompetentanimal AT abdelfattahselim evaluationoftheimmunopathologicalresponsetobcgvaccineinaxenogeneicimmunocompetentanimal AT majedhwakid evaluationoftheimmunopathologicalresponsetobcgvaccineinaxenogeneicimmunocompetentanimal |