Microscopic Evaluation of the Effect of Oral Microbiota on the Development of Bisphosphonate-Related Osteonecrosis of the Jaws in Rats

Objectives: Osteonecrosis of the jaws is a side effect associated with the use of bisphosphonates. Using histologic analysis, this study aimed to evaluate the influence of microbial colonies in the development of osteonecrosis in the jaws of rats subjected to nitrogenous and non-nitrogenous bisphosp...

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Main Authors: Felipe M. Silveira, Adriana Etges, Marcos B. Correa, Ana Carolina U. Vasconcelos
Format: Article
Language:English
Published: Stilus Optimus 2017-01-01
Series:eJournal of Oral Maxillofacial Research
Subjects:
Online Access:http://www.ejomr.org/JOMR/archives/2016/4/e3/v7n4e3ht.htm
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author Felipe M. Silveira
Adriana Etges
Marcos B. Correa
Ana Carolina U. Vasconcelos
author_facet Felipe M. Silveira
Adriana Etges
Marcos B. Correa
Ana Carolina U. Vasconcelos
author_sort Felipe M. Silveira
collection DOAJ
description Objectives: Osteonecrosis of the jaws is a side effect associated with the use of bisphosphonates. Using histologic analysis, this study aimed to evaluate the influence of microbial colonies in the development of osteonecrosis in the jaws of rats subjected to nitrogenous and non-nitrogenous bisphosphonates, undergoing surgical procedures. Material and Methods: Thirty-four rats (Rattus norvegicus, Wistar strain) were allocated randomly into three groups: 12 animals treated with zoledronic acid; 12 animals treated with clodronate; and 10 animals treated with saline. Sixty days after the start of treatment, the animals underwent three extractions of the upper right molars. After 120 days of drug administration, the rats were killed. Histologic analysis was performed on specimens stained with hematoxylin and eosin by the technique of manual counting points using Image-Pro Plus software on images of the right hemimaxilla. Results: Osteonecrosis was induced in the test groups. There was no statistically significant association between the presence of microbial colonies and the presence of non-vital bone (Kruskal-Wallis, P > 0.05). Conclusions: Use of zoledronic acid was associated with non-vital bone and the results suggested that the presence of microbial colonies does not lead to osteonecrosis.
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spelling doaj.art-11ecfcc6b55c4433a6517c6ccdfc9ecf2022-12-22T00:41:19ZengStilus OptimuseJournal of Oral Maxillofacial Research2029-283X2017-01-0174e310.5037/jomr.2016.7403Microscopic Evaluation of the Effect of Oral Microbiota on the Development of Bisphosphonate-Related Osteonecrosis of the Jaws in RatsFelipe M. SilveiraAdriana EtgesMarcos B. CorreaAna Carolina U. VasconcelosObjectives: Osteonecrosis of the jaws is a side effect associated with the use of bisphosphonates. Using histologic analysis, this study aimed to evaluate the influence of microbial colonies in the development of osteonecrosis in the jaws of rats subjected to nitrogenous and non-nitrogenous bisphosphonates, undergoing surgical procedures. Material and Methods: Thirty-four rats (Rattus norvegicus, Wistar strain) were allocated randomly into three groups: 12 animals treated with zoledronic acid; 12 animals treated with clodronate; and 10 animals treated with saline. Sixty days after the start of treatment, the animals underwent three extractions of the upper right molars. After 120 days of drug administration, the rats were killed. Histologic analysis was performed on specimens stained with hematoxylin and eosin by the technique of manual counting points using Image-Pro Plus software on images of the right hemimaxilla. Results: Osteonecrosis was induced in the test groups. There was no statistically significant association between the presence of microbial colonies and the presence of non-vital bone (Kruskal-Wallis, P > 0.05). Conclusions: Use of zoledronic acid was associated with non-vital bone and the results suggested that the presence of microbial colonies does not lead to osteonecrosis.http://www.ejomr.org/JOMR/archives/2016/4/e3/v7n4e3ht.htmbisphosphonate-associated osteonecrosis of the jawdiphosphonatesinfection
spellingShingle Felipe M. Silveira
Adriana Etges
Marcos B. Correa
Ana Carolina U. Vasconcelos
Microscopic Evaluation of the Effect of Oral Microbiota on the Development of Bisphosphonate-Related Osteonecrosis of the Jaws in Rats
eJournal of Oral Maxillofacial Research
bisphosphonate-associated osteonecrosis of the jaw
diphosphonates
infection
title Microscopic Evaluation of the Effect of Oral Microbiota on the Development of Bisphosphonate-Related Osteonecrosis of the Jaws in Rats
title_full Microscopic Evaluation of the Effect of Oral Microbiota on the Development of Bisphosphonate-Related Osteonecrosis of the Jaws in Rats
title_fullStr Microscopic Evaluation of the Effect of Oral Microbiota on the Development of Bisphosphonate-Related Osteonecrosis of the Jaws in Rats
title_full_unstemmed Microscopic Evaluation of the Effect of Oral Microbiota on the Development of Bisphosphonate-Related Osteonecrosis of the Jaws in Rats
title_short Microscopic Evaluation of the Effect of Oral Microbiota on the Development of Bisphosphonate-Related Osteonecrosis of the Jaws in Rats
title_sort microscopic evaluation of the effect of oral microbiota on the development of bisphosphonate related osteonecrosis of the jaws in rats
topic bisphosphonate-associated osteonecrosis of the jaw
diphosphonates
infection
url http://www.ejomr.org/JOMR/archives/2016/4/e3/v7n4e3ht.htm
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