Gibberellin-Mediated Sensitivity of Rice Roots to Aluminum Stress
Aluminum toxicity poses a significant constraint on crop production in acidic soils. While phytohormones are recognized for their pivotal role in mediating plant responses to aluminum stress, the specific involvement of gibberellin (GA) in regulating aluminum tolerance remains unexplored. In this st...
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MDPI AG
2024-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/4/543 |
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| author | Long Lu Xinyu Chen Qinyan Tan Wenqian Li Yanyan Sun Zaoli Zhang Yuanyuan Song Rensen Zeng |
| author_facet | Long Lu Xinyu Chen Qinyan Tan Wenqian Li Yanyan Sun Zaoli Zhang Yuanyuan Song Rensen Zeng |
| author_sort | Long Lu |
| collection | DOAJ |
| description | Aluminum toxicity poses a significant constraint on crop production in acidic soils. While phytohormones are recognized for their pivotal role in mediating plant responses to aluminum stress, the specific involvement of gibberellin (GA) in regulating aluminum tolerance remains unexplored. In this study, we demonstrate that external GA exacerbates the inhibitory impact of aluminum stress on root growth of rice seedlings, concurrently promoting reactive oxygen species (ROS) accumulation. Furthermore, rice plants overexpressing the GA synthesis gene <i>SD1</i> exhibit enhanced sensitivity to aluminum stress. In contrast, the <i>slr1</i> gain-of-function mutant, characterized by impeded GA signaling, displays enhanced tolerance to aluminum stress, suggesting the negative regulatory role of GA in rice resistance to aluminum-induced toxicity. We also reveal that GA application suppresses the expression of crucial aluminum tolerance genes in rice, including <i>Al resistance transcription factor 1</i> (<i>ART1</i>), <i>Nramp aluminum transporter 1</i> (<i>OsNramp4</i>), and <i>Sensitive to Aluminum 1</i> (<i>SAL1</i>). Conversely, the <i>slr1</i> mutant exhibits up-regulated expression of these genes compared to the wild type. In summary, our results shed light on the inhibitory effect of GA in rice resistance to aluminum stress, contributing to a theoretical foundation for unraveling the intricate mechanisms of plant hormones in regulating aluminum tolerance. |
| first_indexed | 2024-03-07T22:16:34Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-07T22:16:34Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-11edffab78db449d8b4725da94478d902024-02-23T15:32:02ZengMDPI AGPlants2223-77472024-02-0113454310.3390/plants13040543Gibberellin-Mediated Sensitivity of Rice Roots to Aluminum StressLong Lu0Xinyu Chen1Qinyan Tan2Wenqian Li3Yanyan Sun4Zaoli Zhang5Yuanyuan Song6Rensen Zeng7Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaAluminum toxicity poses a significant constraint on crop production in acidic soils. While phytohormones are recognized for their pivotal role in mediating plant responses to aluminum stress, the specific involvement of gibberellin (GA) in regulating aluminum tolerance remains unexplored. In this study, we demonstrate that external GA exacerbates the inhibitory impact of aluminum stress on root growth of rice seedlings, concurrently promoting reactive oxygen species (ROS) accumulation. Furthermore, rice plants overexpressing the GA synthesis gene <i>SD1</i> exhibit enhanced sensitivity to aluminum stress. In contrast, the <i>slr1</i> gain-of-function mutant, characterized by impeded GA signaling, displays enhanced tolerance to aluminum stress, suggesting the negative regulatory role of GA in rice resistance to aluminum-induced toxicity. We also reveal that GA application suppresses the expression of crucial aluminum tolerance genes in rice, including <i>Al resistance transcription factor 1</i> (<i>ART1</i>), <i>Nramp aluminum transporter 1</i> (<i>OsNramp4</i>), and <i>Sensitive to Aluminum 1</i> (<i>SAL1</i>). Conversely, the <i>slr1</i> mutant exhibits up-regulated expression of these genes compared to the wild type. In summary, our results shed light on the inhibitory effect of GA in rice resistance to aluminum stress, contributing to a theoretical foundation for unraveling the intricate mechanisms of plant hormones in regulating aluminum tolerance.https://www.mdpi.com/2223-7747/13/4/543aluminum toxicityricegibberellin biosynthesis and signalingROS accumulation |
| spellingShingle | Long Lu Xinyu Chen Qinyan Tan Wenqian Li Yanyan Sun Zaoli Zhang Yuanyuan Song Rensen Zeng Gibberellin-Mediated Sensitivity of Rice Roots to Aluminum Stress Plants aluminum toxicity rice gibberellin biosynthesis and signaling ROS accumulation |
| title | Gibberellin-Mediated Sensitivity of Rice Roots to Aluminum Stress |
| title_full | Gibberellin-Mediated Sensitivity of Rice Roots to Aluminum Stress |
| title_fullStr | Gibberellin-Mediated Sensitivity of Rice Roots to Aluminum Stress |
| title_full_unstemmed | Gibberellin-Mediated Sensitivity of Rice Roots to Aluminum Stress |
| title_short | Gibberellin-Mediated Sensitivity of Rice Roots to Aluminum Stress |
| title_sort | gibberellin mediated sensitivity of rice roots to aluminum stress |
| topic | aluminum toxicity rice gibberellin biosynthesis and signaling ROS accumulation |
| url | https://www.mdpi.com/2223-7747/13/4/543 |
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