miR-331-5p Affects Motility of Thyroid Cancer Cell Lines and Regulates BID Expression
During tumorigenesis, miRNAs with unbalanced expression profiles can increase the threat of disease progression. Here, we focus on the role of miR-331-5p in the pathogenesis of thyroid cancer (TC). In vitro studies were conducted using TC cell lines after the forced expression and silencing of miR-3...
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MDPI AG
2024-03-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/3/658 |
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| author | Francesca Maria Orlandella Esther Imperlini Katia Pane Neila Luciano Mariantonia Braile Anna Elisa De Stefano Paola Lucia Chiara Iervolino Alessandro Ruocco Stefania Orrù Monica Franzese Giuliana Salvatore |
| author_facet | Francesca Maria Orlandella Esther Imperlini Katia Pane Neila Luciano Mariantonia Braile Anna Elisa De Stefano Paola Lucia Chiara Iervolino Alessandro Ruocco Stefania Orrù Monica Franzese Giuliana Salvatore |
| author_sort | Francesca Maria Orlandella |
| collection | DOAJ |
| description | During tumorigenesis, miRNAs with unbalanced expression profiles can increase the threat of disease progression. Here, we focus on the role of miR-331-5p in the pathogenesis of thyroid cancer (TC). In vitro studies were conducted using TC cell lines after the forced expression and silencing of miR-331-5p. Cell proliferation and viability were analyzed via cell counts and colorimetric assays. Cell motility was analyzed via wound healing assays, Transwell migration and invasion assays, and Matrigel Matrix assays. The putative targets of miR-331-5p were unveiled via label-free proteomic screening and then verified using Western blot and luciferase assays. Expression studies were conducted by interrogating The Cancer Genome Atlas (TCGA). We found that ectopic miR-331-5p expression reduces TC cell motility, while miR-331-5p silencing induces the opposite phenotype. Proteomic screening revealed eight putative downregulated targets of miR-331-5p, among which BID was confirmed as a direct target. TCGA data showed the downregulation of miR-331-5p and the upregulation of <i>BID</i> in TC tissues. In summary, deregulation of the miR-331-5p/BID axis could enhance the aggressiveness of TC cell lines, providing new insights into the mechanisms of the progression of this disease and suggesting a potential role of the component factors as possible biomarkers in TC tissues. |
| first_indexed | 2024-04-24T18:30:50Z |
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| id | doaj.art-11eebf39e0864d42b80e08ac3f9b55a1 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-04-24T18:30:50Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-11eebf39e0864d42b80e08ac3f9b55a12024-03-27T13:23:03ZengMDPI AGBiomedicines2227-90592024-03-0112365810.3390/biomedicines12030658miR-331-5p Affects Motility of Thyroid Cancer Cell Lines and Regulates BID ExpressionFrancesca Maria Orlandella0Esther Imperlini1Katia Pane2Neila Luciano3Mariantonia Braile4Anna Elisa De Stefano5Paola Lucia Chiara Iervolino6Alessandro Ruocco7Stefania Orrù8Monica Franzese9Giuliana Salvatore10Dipartimento delle Scienze Mediche, Motorie e del Benessere, Università degli Studi di Napoli “Parthenope”, 80133 Naples, ItalyDepartment for Innovation in Biological, Agrofood and Forest Systems, University of Tuscia, 01100 Viterbo, ItalyIRCCS SYNLAB SDN, 80143 Naples, ItalyDipartimento di Scienze Biomediche Avanzate, Università degli Studi di Napoli “Federico II”, 80131 Naples, ItalyIRCCS SYNLAB SDN, 80143 Naples, ItalyDipartimento delle Scienze Mediche, Motorie e del Benessere, Università degli Studi di Napoli “Parthenope”, 80133 Naples, ItalyDipartimento di Scienze Biomediche Avanzate, Università degli Studi di Napoli “Federico II”, 80131 Naples, ItalyCEINGE Biotecnologie Avanzate Franco Salvatore S.c.a.r.l, 80145 Naples, ItalyDipartimento delle Scienze Mediche, Motorie e del Benessere, Università degli Studi di Napoli “Parthenope”, 80133 Naples, ItalyIRCCS SYNLAB SDN, 80143 Naples, ItalyDipartimento delle Scienze Mediche, Motorie e del Benessere, Università degli Studi di Napoli “Parthenope”, 80133 Naples, ItalyDuring tumorigenesis, miRNAs with unbalanced expression profiles can increase the threat of disease progression. Here, we focus on the role of miR-331-5p in the pathogenesis of thyroid cancer (TC). In vitro studies were conducted using TC cell lines after the forced expression and silencing of miR-331-5p. Cell proliferation and viability were analyzed via cell counts and colorimetric assays. Cell motility was analyzed via wound healing assays, Transwell migration and invasion assays, and Matrigel Matrix assays. The putative targets of miR-331-5p were unveiled via label-free proteomic screening and then verified using Western blot and luciferase assays. Expression studies were conducted by interrogating The Cancer Genome Atlas (TCGA). We found that ectopic miR-331-5p expression reduces TC cell motility, while miR-331-5p silencing induces the opposite phenotype. Proteomic screening revealed eight putative downregulated targets of miR-331-5p, among which BID was confirmed as a direct target. TCGA data showed the downregulation of miR-331-5p and the upregulation of <i>BID</i> in TC tissues. In summary, deregulation of the miR-331-5p/BID axis could enhance the aggressiveness of TC cell lines, providing new insights into the mechanisms of the progression of this disease and suggesting a potential role of the component factors as possible biomarkers in TC tissues.https://www.mdpi.com/2227-9059/12/3/658miR-331-5pBIDthyroid carcinomamotility |
| spellingShingle | Francesca Maria Orlandella Esther Imperlini Katia Pane Neila Luciano Mariantonia Braile Anna Elisa De Stefano Paola Lucia Chiara Iervolino Alessandro Ruocco Stefania Orrù Monica Franzese Giuliana Salvatore miR-331-5p Affects Motility of Thyroid Cancer Cell Lines and Regulates BID Expression Biomedicines miR-331-5p BID thyroid carcinoma motility |
| title | miR-331-5p Affects Motility of Thyroid Cancer Cell Lines and Regulates BID Expression |
| title_full | miR-331-5p Affects Motility of Thyroid Cancer Cell Lines and Regulates BID Expression |
| title_fullStr | miR-331-5p Affects Motility of Thyroid Cancer Cell Lines and Regulates BID Expression |
| title_full_unstemmed | miR-331-5p Affects Motility of Thyroid Cancer Cell Lines and Regulates BID Expression |
| title_short | miR-331-5p Affects Motility of Thyroid Cancer Cell Lines and Regulates BID Expression |
| title_sort | mir 331 5p affects motility of thyroid cancer cell lines and regulates bid expression |
| topic | miR-331-5p BID thyroid carcinoma motility |
| url | https://www.mdpi.com/2227-9059/12/3/658 |
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