Characterizing the bacterial communities associated with Mediterranean sponges: a metataxonomic analysis
The oceans cover over 70% of our planet, hosting a biodiversity of tremendous wealth. Sponges are one of the major ecosystem engineers on the seafloor, providing a habitat for a wide variety of species to be considered a good source of bioactive compounds. In this study, a metataxonomic approach was...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1295459/full |
| _version_ | 1827383930120044544 |
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| author | Roberta Esposito Serena Federico Serena Federico Michele Sonnessa Sofia Reddel Marco Bertolino Nadia Ruocco Giacomo Zagami Marco Giovine Marina Pozzolini Marco Guida Valerio Zupo Maria Costantini |
| author_facet | Roberta Esposito Serena Federico Serena Federico Michele Sonnessa Sofia Reddel Marco Bertolino Nadia Ruocco Giacomo Zagami Marco Giovine Marina Pozzolini Marco Guida Valerio Zupo Maria Costantini |
| author_sort | Roberta Esposito |
| collection | DOAJ |
| description | The oceans cover over 70% of our planet, hosting a biodiversity of tremendous wealth. Sponges are one of the major ecosystem engineers on the seafloor, providing a habitat for a wide variety of species to be considered a good source of bioactive compounds. In this study, a metataxonomic approach was employed to describe the bacterial communities of the sponges collected from Faro Lake (Sicily) and Porto Paone (Gulf of Naples). Morphological analysis and amplification of the conserved molecular markers, including 18S and 28S (RNA ribosomal genes), CO1 (mitochondrial cytochrome oxidase subunit 1), and ITS (internal transcribed spacer), allowed the identification of four sponges. Metataxonomic analysis of sponges revealed a large number of amplicon sequence variants (ASVs) belonging to the phyla Proteobacteria, Cloroflexi, Dadabacteria, and Poribacteria. In particular, Myxilla (Myxilla) rosacea and Clathria (Clathria) toxivaria displayed several classes such as Alphaproteobacteria, Dehalococcoidia, Gammaproteobacteria, Cyanobacteria, and Bacteroidia. On the other hand, the sponges Ircinia oros and Cacospongia mollior hosted bacteria belonging to the classes Dadabacteriia, Anaerolineae, Acidimicrobiia, Nitrospiria, and Poribacteria. Moreover, for the first time, the presence of Rhizobiaceae bacteria was revealed in the sponge M. (Myxilla) rosacea, which was mainly associated with soil and plants and involved in biological nitrogen fixation. |
| first_indexed | 2024-03-08T14:48:40Z |
| format | Article |
| id | doaj.art-11eec9a76fab4780b3509ed86f5a85b0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T14:48:40Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-11eec9a76fab4780b3509ed86f5a85b02024-01-11T04:42:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-01-011410.3389/fmicb.2023.12954591295459Characterizing the bacterial communities associated with Mediterranean sponges: a metataxonomic analysisRoberta Esposito0Serena Federico1Serena Federico2Michele Sonnessa3Sofia Reddel4Marco Bertolino5Nadia Ruocco6Giacomo Zagami7Marco Giovine8Marina Pozzolini9Marco Guida10Valerio Zupo11Maria Costantini12Department of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Napoli, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Napoli, ItalyDepartment of Earth, Environmental and Life Sciences, University of Genoa, Genoa, ItalyBio-Fab Research Srl, Rome, ItalyBio-Fab Research Srl, Rome, ItalyDepartment of Earth, Environmental and Life Sciences, University of Genoa, Genoa, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Calabria Marine Centre, Amendolara, ItalyDipartimento Di Scienze Biologiche, Chimiche, Farmaceutiche Ed Ambientali, Università Di Messina, Messina, ItalyDepartment of Earth, Environmental and Life Sciences, University of Genoa, Genoa, ItalyDepartment of Earth, Environmental and Life Sciences, University of Genoa, Genoa, ItalyDepartment of Biology, University of Naples Federico II, Complesso Universitario di Monte Sant'Angelo, Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Ischia Marine Centre, Naples, ItalyDepartment of Ecosustainable Marine Biotechnology, Stazione Zoologica Anton Dohrn, Napoli, ItalyThe oceans cover over 70% of our planet, hosting a biodiversity of tremendous wealth. Sponges are one of the major ecosystem engineers on the seafloor, providing a habitat for a wide variety of species to be considered a good source of bioactive compounds. In this study, a metataxonomic approach was employed to describe the bacterial communities of the sponges collected from Faro Lake (Sicily) and Porto Paone (Gulf of Naples). Morphological analysis and amplification of the conserved molecular markers, including 18S and 28S (RNA ribosomal genes), CO1 (mitochondrial cytochrome oxidase subunit 1), and ITS (internal transcribed spacer), allowed the identification of four sponges. Metataxonomic analysis of sponges revealed a large number of amplicon sequence variants (ASVs) belonging to the phyla Proteobacteria, Cloroflexi, Dadabacteria, and Poribacteria. In particular, Myxilla (Myxilla) rosacea and Clathria (Clathria) toxivaria displayed several classes such as Alphaproteobacteria, Dehalococcoidia, Gammaproteobacteria, Cyanobacteria, and Bacteroidia. On the other hand, the sponges Ircinia oros and Cacospongia mollior hosted bacteria belonging to the classes Dadabacteriia, Anaerolineae, Acidimicrobiia, Nitrospiria, and Poribacteria. Moreover, for the first time, the presence of Rhizobiaceae bacteria was revealed in the sponge M. (Myxilla) rosacea, which was mainly associated with soil and plants and involved in biological nitrogen fixation.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1295459/fullbacteriametataxonomic analysismolecular identificationmorphological identificationsponges |
| spellingShingle | Roberta Esposito Serena Federico Serena Federico Michele Sonnessa Sofia Reddel Marco Bertolino Nadia Ruocco Giacomo Zagami Marco Giovine Marina Pozzolini Marco Guida Valerio Zupo Maria Costantini Characterizing the bacterial communities associated with Mediterranean sponges: a metataxonomic analysis Frontiers in Microbiology bacteria metataxonomic analysis molecular identification morphological identification sponges |
| title | Characterizing the bacterial communities associated with Mediterranean sponges: a metataxonomic analysis |
| title_full | Characterizing the bacterial communities associated with Mediterranean sponges: a metataxonomic analysis |
| title_fullStr | Characterizing the bacterial communities associated with Mediterranean sponges: a metataxonomic analysis |
| title_full_unstemmed | Characterizing the bacterial communities associated with Mediterranean sponges: a metataxonomic analysis |
| title_short | Characterizing the bacterial communities associated with Mediterranean sponges: a metataxonomic analysis |
| title_sort | characterizing the bacterial communities associated with mediterranean sponges a metataxonomic analysis |
| topic | bacteria metataxonomic analysis molecular identification morphological identification sponges |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1295459/full |
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