Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model
Marine plants are important sources of pharmacologically active metabolites. The aim of the present work was to evaluate the cytotoxic and antitumor activity of a polyphenolic fraction obtained from Thalassia testudinum marine plant and thalassiolin B in human colorectal cancer cells. Human cancer c...
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2020.592985/full |
| _version_ | 1818647576500502528 |
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| author | Livan Delgado-Roche Livan Delgado-Roche Kethia González Fernando Mesta Beatriz Couder Zaira Tavarez Ruby Zavala Ivones Hernandez Gabino Garrido Idania Rodeiro Wim Vanden Berghe |
| author_facet | Livan Delgado-Roche Livan Delgado-Roche Kethia González Fernando Mesta Beatriz Couder Zaira Tavarez Ruby Zavala Ivones Hernandez Gabino Garrido Idania Rodeiro Wim Vanden Berghe |
| author_sort | Livan Delgado-Roche |
| collection | DOAJ |
| description | Marine plants are important sources of pharmacologically active metabolites. The aim of the present work was to evaluate the cytotoxic and antitumor activity of a polyphenolic fraction obtained from Thalassia testudinum marine plant and thalassiolin B in human colorectal cancer cells. Human cancer cell lines, including HCT15, HCT116, SW260, and HT29 were treated with tested products for cytotoxicity evaluation by crystal violet assay. The potential proapoptotic effect of these natural products was assessed by flow cytometry in HCT15 cells at 48 h using Annexin V-FITC/propidium iodide. In addition, reactive oxygen species (ROS) generation was measured by fluorescence using DCFH-DA staining, and sulfhydryl concentration by spectrophotometry. The in vivo antitumor activity of the polyphenolic fraction (25 mg/kg) was evaluated in a xenograft model in nu/nu mice. In vivo proapoptotic effect was also evaluated by immunohistochemistry using anti-caspase 3 and anti-Bcl-2 antibodies. The results showed that tested products exert colorectal cancer cell cytotoxicity. Besides, the tested products induced a significant increase (p < 0.05) of intracellular ROS generation, and a depletion of sulfhydryl concentration in HCT15 cells. The polyphenolic fraction arrested tumor growth and induced apoptosis in the xenograft mice model. These results demonstrate the cytotoxic activity of T. testudinum metabolites associated, at least, with ROS overproduction and pro-apoptotic effects. Here we demonstrated for the first time the antitumor activity of a T. testudinum polar extract in a xenograft mice model. These results suggest the potential use of T. testudinum marine plant metabolites as adjuvant treatment in cancer therapy. |
| first_indexed | 2024-12-17T01:04:44Z |
| format | Article |
| id | doaj.art-11ef240ae4b0423db23b276f5e8cdb26 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-17T01:04:44Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-11ef240ae4b0423db23b276f5e8cdb262022-12-21T22:09:19ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-11-011110.3389/fphar.2020.592985592985Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse ModelLivan Delgado-Roche0Livan Delgado-Roche1Kethia González2Fernando Mesta3Beatriz Couder4Zaira Tavarez5Ruby Zavala6Ivones Hernandez7Gabino Garrido8Idania Rodeiro9Wim Vanden Berghe10Dirección Médica, Laboratorios Liomont S.A. de C.V., Ciudad de México, MéxicoInstituto de Ciencias del Mar (ICIMAR), La Habana, CubaInstituto de Ciencias del Mar (ICIMAR), La Habana, CubaEscuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Ciudad de México, MéxicoUniversidad Nacional Autónoma de Mexico, Ciudad Universitaria, Ciudad de México, MéxicoUniversidad Nacional Autónoma de Mexico, Ciudad Universitaria, Ciudad de México, MéxicoUniversidad Nacional Autónoma de Mexico, Ciudad Universitaria, Ciudad de México, MéxicoInstituto de Ciencias del Mar (ICIMAR), La Habana, CubaDepartamento de Ciencias Farmacéuticas, Facultad de Ciencias, Universidad de Católica del Norte, Antofagasta, ChileInstituto de Ciencias del Mar (ICIMAR), La Habana, CubaPPES Lab, Proteinchemistry, Proteomics and Epigenetic Signaling, Department of Biomedical Sciences, University of Antwerp, Antwerp, BelgiumMarine plants are important sources of pharmacologically active metabolites. The aim of the present work was to evaluate the cytotoxic and antitumor activity of a polyphenolic fraction obtained from Thalassia testudinum marine plant and thalassiolin B in human colorectal cancer cells. Human cancer cell lines, including HCT15, HCT116, SW260, and HT29 were treated with tested products for cytotoxicity evaluation by crystal violet assay. The potential proapoptotic effect of these natural products was assessed by flow cytometry in HCT15 cells at 48 h using Annexin V-FITC/propidium iodide. In addition, reactive oxygen species (ROS) generation was measured by fluorescence using DCFH-DA staining, and sulfhydryl concentration by spectrophotometry. The in vivo antitumor activity of the polyphenolic fraction (25 mg/kg) was evaluated in a xenograft model in nu/nu mice. In vivo proapoptotic effect was also evaluated by immunohistochemistry using anti-caspase 3 and anti-Bcl-2 antibodies. The results showed that tested products exert colorectal cancer cell cytotoxicity. Besides, the tested products induced a significant increase (p < 0.05) of intracellular ROS generation, and a depletion of sulfhydryl concentration in HCT15 cells. The polyphenolic fraction arrested tumor growth and induced apoptosis in the xenograft mice model. These results demonstrate the cytotoxic activity of T. testudinum metabolites associated, at least, with ROS overproduction and pro-apoptotic effects. Here we demonstrated for the first time the antitumor activity of a T. testudinum polar extract in a xenograft mice model. These results suggest the potential use of T. testudinum marine plant metabolites as adjuvant treatment in cancer therapy.https://www.frontiersin.org/articles/10.3389/fphar.2020.592985/fullT. testudinumpolyphenolsthalassiolin Bcolorectal cancerreactive oxygen speciesapoptosis |
| spellingShingle | Livan Delgado-Roche Livan Delgado-Roche Kethia González Fernando Mesta Beatriz Couder Zaira Tavarez Ruby Zavala Ivones Hernandez Gabino Garrido Idania Rodeiro Wim Vanden Berghe Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model Frontiers in Pharmacology T. testudinum polyphenols thalassiolin B colorectal cancer reactive oxygen species apoptosis |
| title | Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model |
| title_full | Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model |
| title_fullStr | Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model |
| title_full_unstemmed | Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model |
| title_short | Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model |
| title_sort | polyphenolic fraction obtained from thalassia testudinum marine plant and thalassiolin b exert cytotoxic effects in colorectal cancer cells and arrest tumor progression in a xenograft mouse model |
| topic | T. testudinum polyphenols thalassiolin B colorectal cancer reactive oxygen species apoptosis |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2020.592985/full |
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