The major surface-associated saccharides of Klebsiella pneumoniae contribute to host cell association.
Analysing the pathogenic mechanisms of a bacterium requires an understanding of the composition of the bacterial cell surface. The bacterial surface provides the first barrier against innate immune mechanisms as well as mediating attachment to cells/surfaces to resist clearance. We utilised a series...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2583945?pdf=render |
| _version_ | 1818150540408782848 |
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| author | Abigail Clements Fabien Gaboriaud Jérôme F L Duval Jacinta L Farn Adam W Jenney Trevor Lithgow Odilia L C Wijburg Elizabeth L Hartland Richard A Strugnell |
| author_facet | Abigail Clements Fabien Gaboriaud Jérôme F L Duval Jacinta L Farn Adam W Jenney Trevor Lithgow Odilia L C Wijburg Elizabeth L Hartland Richard A Strugnell |
| author_sort | Abigail Clements |
| collection | DOAJ |
| description | Analysing the pathogenic mechanisms of a bacterium requires an understanding of the composition of the bacterial cell surface. The bacterial surface provides the first barrier against innate immune mechanisms as well as mediating attachment to cells/surfaces to resist clearance. We utilised a series of Klebsiella pneumoniae mutants in which the two major polysaccharide layers, capsule and lipopolysaccharide (LPS), were absent or truncated, to investigate the ability of these layers to protect against innate immune mechanisms and to associate with eukaryotic cells. The capsule alone was found to be essential for resistance to complement mediated killing while both capsule and LPS were involved in cell-association, albeit through different mechanisms. The capsule impeded cell-association while the LPS saccharides increased cell-association in a non-specific manner. The electrohydrodynamic characteristics of the strains suggested the differing interaction of each bacterial strain with eukaryotic cells could be partly explained by the charge density displayed by the outermost polysaccharide layer. This highlights the importance of considering not only specific adhesin:ligand interactions commonly studied in adherence assays but also the initial non-specific interactions governed largely by the electrostatic interaction forces. |
| first_indexed | 2024-12-11T13:24:33Z |
| format | Article |
| id | doaj.art-11efc418e1a9431eb651f3a786902b0d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T13:24:33Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-11efc418e1a9431eb651f3a786902b0d2022-12-22T01:05:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01311e381710.1371/journal.pone.0003817The major surface-associated saccharides of Klebsiella pneumoniae contribute to host cell association.Abigail ClementsFabien GaboriaudJérôme F L DuvalJacinta L FarnAdam W JenneyTrevor LithgowOdilia L C WijburgElizabeth L HartlandRichard A StrugnellAnalysing the pathogenic mechanisms of a bacterium requires an understanding of the composition of the bacterial cell surface. The bacterial surface provides the first barrier against innate immune mechanisms as well as mediating attachment to cells/surfaces to resist clearance. We utilised a series of Klebsiella pneumoniae mutants in which the two major polysaccharide layers, capsule and lipopolysaccharide (LPS), were absent or truncated, to investigate the ability of these layers to protect against innate immune mechanisms and to associate with eukaryotic cells. The capsule alone was found to be essential for resistance to complement mediated killing while both capsule and LPS were involved in cell-association, albeit through different mechanisms. The capsule impeded cell-association while the LPS saccharides increased cell-association in a non-specific manner. The electrohydrodynamic characteristics of the strains suggested the differing interaction of each bacterial strain with eukaryotic cells could be partly explained by the charge density displayed by the outermost polysaccharide layer. This highlights the importance of considering not only specific adhesin:ligand interactions commonly studied in adherence assays but also the initial non-specific interactions governed largely by the electrostatic interaction forces.http://europepmc.org/articles/PMC2583945?pdf=render |
| spellingShingle | Abigail Clements Fabien Gaboriaud Jérôme F L Duval Jacinta L Farn Adam W Jenney Trevor Lithgow Odilia L C Wijburg Elizabeth L Hartland Richard A Strugnell The major surface-associated saccharides of Klebsiella pneumoniae contribute to host cell association. PLoS ONE |
| title | The major surface-associated saccharides of Klebsiella pneumoniae contribute to host cell association. |
| title_full | The major surface-associated saccharides of Klebsiella pneumoniae contribute to host cell association. |
| title_fullStr | The major surface-associated saccharides of Klebsiella pneumoniae contribute to host cell association. |
| title_full_unstemmed | The major surface-associated saccharides of Klebsiella pneumoniae contribute to host cell association. |
| title_short | The major surface-associated saccharides of Klebsiella pneumoniae contribute to host cell association. |
| title_sort | major surface associated saccharides of klebsiella pneumoniae contribute to host cell association |
| url | http://europepmc.org/articles/PMC2583945?pdf=render |
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