CaHSP18.1a, a Small Heat Shock Protein from Pepper (<i>Capsicum annuum</i> L.), Positively Responds to Heat, Drought, and Salt Tolerance
Pepper is a thermophilic crop, shallow-rooted plant that is often severely affected by abiotic stresses such as heat, salt, and drought. The growth and development of pepper is seriously affected by adverse stresses, resulting in decreases in the yield and quality of pepper crops. Small heat shock p...
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MDPI AG
2021-05-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/5/117 |
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| author | Yan-Li Liu Shuai Liu Jing-Jing Xiao Guo-Xin Cheng Haq Saeed ul Zhen-Hui Gong |
| author_facet | Yan-Li Liu Shuai Liu Jing-Jing Xiao Guo-Xin Cheng Haq Saeed ul Zhen-Hui Gong |
| author_sort | Yan-Li Liu |
| collection | DOAJ |
| description | Pepper is a thermophilic crop, shallow-rooted plant that is often severely affected by abiotic stresses such as heat, salt, and drought. The growth and development of pepper is seriously affected by adverse stresses, resulting in decreases in the yield and quality of pepper crops. Small heat shock proteins (s HSPs) play a crucial role in protecting plant cells against various stresses. A previous study in our laboratory showed that the expression level of <i>CaHSP18.1a</i> was highly induced by heat stress, but the function and mechanism of CaHSP18.1a responding to abiotic stresses is not clear. In this study, we first analyzed the expression of <i>CaHSP18.1a</i> in the thermo-sensitive B6 line and thermo-tolerant R9 line and demonstrated that the transcription of <i>CaHSP18.1a</i> was strongly induced by heat stress, salt, and drought stress in both R9 and B6, and that the response is more intense and earlier in the R9 line. In the R9 line, the silencing of <i>CaHSP18.1a</i> decreased resistance to heat, drought, and salt stresses. The silencing of <i>CaHSP18.1a</i> resulted in significant increases in relative electrolyte leakage (REL) and malonaldehyde (MDA) contents, while total chlorophyll content decreased under heat, salt, and drought stresses. Overexpression analyses of <i>CaHSP18.1a</i> in transgenic <i>Arabidopsis</i> further confirmed that <i>CaHSP18.1a</i> functions positively in resistance to heat, drought, and salt stresses. The transgenic <i>Arabidopsis</i> had higherchlorophyll content and activities of superoxide dismutase, catalase, and ascorbate peroxidase than the wild type (WT). However, the relative conductivity and MDA content were decreased in transgenic Arabidopsis compared to the wild type (WT). We further showed that the CaHSP18.1a protein is localized to the cell membrane. These results indicate CaHSP18.1a may act as a positive regulator of responses to abiotic stresses. |
| first_indexed | 2024-03-10T11:16:47Z |
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| language | English |
| last_indexed | 2024-03-10T11:16:47Z |
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| spelling | doaj.art-11f2cfe66b5b419fbae1d2f448eff3972023-11-21T20:20:05ZengMDPI AGHorticulturae2311-75242021-05-017511710.3390/horticulturae7050117CaHSP18.1a, a Small Heat Shock Protein from Pepper (<i>Capsicum annuum</i> L.), Positively Responds to Heat, Drought, and Salt ToleranceYan-Li Liu0Shuai Liu1Jing-Jing Xiao2Guo-Xin Cheng3Haq Saeed ul4Zhen-Hui Gong5College of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling 712100, ChinaPepper is a thermophilic crop, shallow-rooted plant that is often severely affected by abiotic stresses such as heat, salt, and drought. The growth and development of pepper is seriously affected by adverse stresses, resulting in decreases in the yield and quality of pepper crops. Small heat shock proteins (s HSPs) play a crucial role in protecting plant cells against various stresses. A previous study in our laboratory showed that the expression level of <i>CaHSP18.1a</i> was highly induced by heat stress, but the function and mechanism of CaHSP18.1a responding to abiotic stresses is not clear. In this study, we first analyzed the expression of <i>CaHSP18.1a</i> in the thermo-sensitive B6 line and thermo-tolerant R9 line and demonstrated that the transcription of <i>CaHSP18.1a</i> was strongly induced by heat stress, salt, and drought stress in both R9 and B6, and that the response is more intense and earlier in the R9 line. In the R9 line, the silencing of <i>CaHSP18.1a</i> decreased resistance to heat, drought, and salt stresses. The silencing of <i>CaHSP18.1a</i> resulted in significant increases in relative electrolyte leakage (REL) and malonaldehyde (MDA) contents, while total chlorophyll content decreased under heat, salt, and drought stresses. Overexpression analyses of <i>CaHSP18.1a</i> in transgenic <i>Arabidopsis</i> further confirmed that <i>CaHSP18.1a</i> functions positively in resistance to heat, drought, and salt stresses. The transgenic <i>Arabidopsis</i> had higherchlorophyll content and activities of superoxide dismutase, catalase, and ascorbate peroxidase than the wild type (WT). However, the relative conductivity and MDA content were decreased in transgenic Arabidopsis compared to the wild type (WT). We further showed that the CaHSP18.1a protein is localized to the cell membrane. These results indicate CaHSP18.1a may act as a positive regulator of responses to abiotic stresses.https://www.mdpi.com/2311-7524/7/5/117<i>CaHSP18.1a</i>gene silencingtransgenic <i>Arabidopsis</i>heat stresspeppergene expression |
| spellingShingle | Yan-Li Liu Shuai Liu Jing-Jing Xiao Guo-Xin Cheng Haq Saeed ul Zhen-Hui Gong CaHSP18.1a, a Small Heat Shock Protein from Pepper (<i>Capsicum annuum</i> L.), Positively Responds to Heat, Drought, and Salt Tolerance Horticulturae <i>CaHSP18.1a</i> gene silencing transgenic <i>Arabidopsis</i> heat stress pepper gene expression |
| title | CaHSP18.1a, a Small Heat Shock Protein from Pepper (<i>Capsicum annuum</i> L.), Positively Responds to Heat, Drought, and Salt Tolerance |
| title_full | CaHSP18.1a, a Small Heat Shock Protein from Pepper (<i>Capsicum annuum</i> L.), Positively Responds to Heat, Drought, and Salt Tolerance |
| title_fullStr | CaHSP18.1a, a Small Heat Shock Protein from Pepper (<i>Capsicum annuum</i> L.), Positively Responds to Heat, Drought, and Salt Tolerance |
| title_full_unstemmed | CaHSP18.1a, a Small Heat Shock Protein from Pepper (<i>Capsicum annuum</i> L.), Positively Responds to Heat, Drought, and Salt Tolerance |
| title_short | CaHSP18.1a, a Small Heat Shock Protein from Pepper (<i>Capsicum annuum</i> L.), Positively Responds to Heat, Drought, and Salt Tolerance |
| title_sort | cahsp18 1a a small heat shock protein from pepper i capsicum annuum i l positively responds to heat drought and salt tolerance |
| topic | <i>CaHSP18.1a</i> gene silencing transgenic <i>Arabidopsis</i> heat stress pepper gene expression |
| url | https://www.mdpi.com/2311-7524/7/5/117 |
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