POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOSTICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUS
Aim. Demonstrate the possibility of using nested PCR method for determination of Varicella Zoster virus (VZV) in clinical samples of peripheral blood of patients. Materials and methods. Material from 35 patients with clinical manifestations of herpes zoster and control group of 20 healthy donors was...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2015-06-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/view/14023 |
| _version_ | 1797782183864172544 |
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| author | Kh. F Fam E. A Borovikova A. V Sidorov A. V Karataeva T. P Antonova V. V Zverev |
| author_facet | Kh. F Fam E. A Borovikova A. V Sidorov A. V Karataeva T. P Antonova V. V Zverev |
| author_sort | Kh. F Fam |
| collection | DOAJ |
| description | Aim. Demonstrate the possibility of using nested PCR method for determination of Varicella Zoster virus (VZV) in clinical samples of peripheral blood of patients. Materials and methods. Material from 35 patients with clinical manifestations of herpes zoster and control group of 20 healthy donors was used in the study. Monocyte fraction of venous blood cells, pretreated with heparin, was isolated by centrifugation in ficoll-verografin density gradient, total DNA was then isolated from cells by phenol-chloroform extraction with subsequent precipitation with alcohol. Polymerase chain reaction was carried out in thermocyclers Tercyc and TProfessional Gradient (Biometra), amplified DNA was analyzed by electrophoresis on 1.6% agarose gel in the presence of ethidium bromide. Results. Data on detection of viral DNA in blood monocytes in 17 (49%) of ill patients, as well as in 1 (out of 20 in control group) practically healthy donor were obtained. A possibility of a subclinical reactivation of the virus is discussed in the latter case. Conclusion. A possibility of viral DNA determination in monocytes of patient blood without using expensive equipment is shown, that could find application in clinical practice, especially for diagnostics of patients with non-characteristic clinical manifestations, as well as patients with subclinical forms of the disease. |
| first_indexed | 2024-03-13T00:07:25Z |
| format | Article |
| id | doaj.art-11f7bc7858f24f2a872972be9eb3cb39 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-13T00:07:25Z |
| publishDate | 2015-06-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-11f7bc7858f24f2a872972be9eb3cb392023-07-12T20:20:08ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132015-06-0192325302449POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOSTICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUSKh. F Fam0E. A Borovikova1A. V Sidorov2A. V Karataeva3T. P Antonova4V. V Zverev5Mechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraMechnikov Research Institute of Vaccines and SeraAim. Demonstrate the possibility of using nested PCR method for determination of Varicella Zoster virus (VZV) in clinical samples of peripheral blood of patients. Materials and methods. Material from 35 patients with clinical manifestations of herpes zoster and control group of 20 healthy donors was used in the study. Monocyte fraction of venous blood cells, pretreated with heparin, was isolated by centrifugation in ficoll-verografin density gradient, total DNA was then isolated from cells by phenol-chloroform extraction with subsequent precipitation with alcohol. Polymerase chain reaction was carried out in thermocyclers Tercyc and TProfessional Gradient (Biometra), amplified DNA was analyzed by electrophoresis on 1.6% agarose gel in the presence of ethidium bromide. Results. Data on detection of viral DNA in blood monocytes in 17 (49%) of ill patients, as well as in 1 (out of 20 in control group) practically healthy donor were obtained. A possibility of a subclinical reactivation of the virus is discussed in the latter case. Conclusion. A possibility of viral DNA determination in monocytes of patient blood without using expensive equipment is shown, that could find application in clinical practice, especially for diagnostics of patients with non-characteristic clinical manifestations, as well as patients with subclinical forms of the disease.https://microbiol.crie.ru/jour/article/view/14023varicella zoster virusdnapcrnested pcr |
| spellingShingle | Kh. F Fam E. A Borovikova A. V Sidorov A. V Karataeva T. P Antonova V. V Zverev POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOSTICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUS Журнал микробиологии, эпидемиологии и иммунобиологии varicella zoster virus dna pcr nested pcr |
| title | POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOSTICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUS |
| title_full | POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOSTICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUS |
| title_fullStr | POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOSTICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUS |
| title_full_unstemmed | POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOSTICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUS |
| title_short | POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOSTICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUS |
| title_sort | possibility of using nested polymerase chain reaction for diagnostics of diseases caused by varicella zoster virus |
| topic | varicella zoster virus dna pcr nested pcr |
| url | https://microbiol.crie.ru/jour/article/view/14023 |
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