Mesenchymal Stem Cells from Familial Alzheimer’s Patients Express MicroRNA Differently
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder and the predominant form of dementia globally. No reliable diagnostic, predictive techniques, or curative interventions are available. MicroRNAs (miRNAs) are vital to controlling gene expression, making them valuable biomarkers for...
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1580 |
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| author | Lory J. Rochín-Hernández Lory S. Rochín-Hernández Mayte L. Padilla-Cristerna Andrea Duarte-García Miguel A. Jiménez-Acosta María P. Figueroa-Corona Marco A. Meraz-Ríos |
| author_facet | Lory J. Rochín-Hernández Lory S. Rochín-Hernández Mayte L. Padilla-Cristerna Andrea Duarte-García Miguel A. Jiménez-Acosta María P. Figueroa-Corona Marco A. Meraz-Ríos |
| author_sort | Lory J. Rochín-Hernández |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is a progressive neurodegenerative disorder and the predominant form of dementia globally. No reliable diagnostic, predictive techniques, or curative interventions are available. MicroRNAs (miRNAs) are vital to controlling gene expression, making them valuable biomarkers for diagnosis and prognosis. This study examines the transcriptome of olfactory ecto-mesenchymal stem cells (MSCs) derived from individuals with the PSEN1(A431E) mutation (Jalisco mutation). The aim is to determine whether this mutation affects the transcriptome and expression profile of miRNAs and their target genes at different stages of asymptomatic, presymptomatic, and symptomatic conditions. Expression microarrays compare the MSCs from mutation carriers with those from healthy donors. The results indicate a distinct variation in the expression of miRNAs and mRNAs among different symptomatologic groups and between individuals with the mutation. Using bioinformatics tools allows us to identify target genes for miRNAs, which in turn affect various biological processes and pathways. These include the cell cycle, senescence, transcription, and pathways involved in regulating the pluripotency of stem cells. These processes are closely linked to inter- and intracellular communication, vital for cellular functioning. These findings can enhance our comprehension and monitoring of the disease’s physiological processes, identify new disorder indicators, and develop innovative treatments and diagnostic tools for preventing or treating AD. |
| first_indexed | 2024-03-08T03:55:17Z |
| format | Article |
| id | doaj.art-11fa380db8804471844b40fe59635784 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:55:17Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-11fa380db8804471844b40fe596357842024-02-09T15:13:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01253158010.3390/ijms25031580Mesenchymal Stem Cells from Familial Alzheimer’s Patients Express MicroRNA DifferentlyLory J. Rochín-Hernández0Lory S. Rochín-Hernández1Mayte L. Padilla-Cristerna2Andrea Duarte-García3Miguel A. Jiménez-Acosta4María P. Figueroa-Corona5Marco A. Meraz-Ríos6Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Biotecnología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoDepartamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Instituto Politécnico Nacional 2508, Ciudad de México 07360, MexicoAlzheimer’s disease (AD) is a progressive neurodegenerative disorder and the predominant form of dementia globally. No reliable diagnostic, predictive techniques, or curative interventions are available. MicroRNAs (miRNAs) are vital to controlling gene expression, making them valuable biomarkers for diagnosis and prognosis. This study examines the transcriptome of olfactory ecto-mesenchymal stem cells (MSCs) derived from individuals with the PSEN1(A431E) mutation (Jalisco mutation). The aim is to determine whether this mutation affects the transcriptome and expression profile of miRNAs and their target genes at different stages of asymptomatic, presymptomatic, and symptomatic conditions. Expression microarrays compare the MSCs from mutation carriers with those from healthy donors. The results indicate a distinct variation in the expression of miRNAs and mRNAs among different symptomatologic groups and between individuals with the mutation. Using bioinformatics tools allows us to identify target genes for miRNAs, which in turn affect various biological processes and pathways. These include the cell cycle, senescence, transcription, and pathways involved in regulating the pluripotency of stem cells. These processes are closely linked to inter- and intracellular communication, vital for cellular functioning. These findings can enhance our comprehension and monitoring of the disease’s physiological processes, identify new disorder indicators, and develop innovative treatments and diagnostic tools for preventing or treating AD.https://www.mdpi.com/1422-0067/25/3/1580miRNAtranscriptomeAlzheimer’s diseasefamilial Alzheimer’s diseaseJalisco mutationPSEN1 |
| spellingShingle | Lory J. Rochín-Hernández Lory S. Rochín-Hernández Mayte L. Padilla-Cristerna Andrea Duarte-García Miguel A. Jiménez-Acosta María P. Figueroa-Corona Marco A. Meraz-Ríos Mesenchymal Stem Cells from Familial Alzheimer’s Patients Express MicroRNA Differently International Journal of Molecular Sciences miRNA transcriptome Alzheimer’s disease familial Alzheimer’s disease Jalisco mutation PSEN1 |
| title | Mesenchymal Stem Cells from Familial Alzheimer’s Patients Express MicroRNA Differently |
| title_full | Mesenchymal Stem Cells from Familial Alzheimer’s Patients Express MicroRNA Differently |
| title_fullStr | Mesenchymal Stem Cells from Familial Alzheimer’s Patients Express MicroRNA Differently |
| title_full_unstemmed | Mesenchymal Stem Cells from Familial Alzheimer’s Patients Express MicroRNA Differently |
| title_short | Mesenchymal Stem Cells from Familial Alzheimer’s Patients Express MicroRNA Differently |
| title_sort | mesenchymal stem cells from familial alzheimer s patients express microrna differently |
| topic | miRNA transcriptome Alzheimer’s disease familial Alzheimer’s disease Jalisco mutation PSEN1 |
| url | https://www.mdpi.com/1422-0067/25/3/1580 |
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