Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles
Growth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed “magnetic targeting”, aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-09-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/8/9/707 |
| _version_ | 1819077048409260032 |
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| author | Michal Marcus Alexandra Smith Ahmad Maswadeh Ziv Shemesh Idan Zak Menachem Motiei Hadas Schori Shlomo Margel Amos Sharoni Orit Shefi |
| author_facet | Michal Marcus Alexandra Smith Ahmad Maswadeh Ziv Shemesh Idan Zak Menachem Motiei Hadas Schori Shlomo Margel Amos Sharoni Orit Shefi |
| author_sort | Michal Marcus |
| collection | DOAJ |
| description | Growth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed “magnetic targeting”, aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for the nervous tissues. Here, we covalently conjugated nerve growth factor to iron oxide nanoparticles (NGF-MNPs) and used controlled magnetic fields to deliver the NGF–MNP complexes to target sites. In order to actuate the magnetic fields a modular magnetic device was designed and fabricated. PC12 cells that were plated homogenously in culture were differentiated selectively only in targeted sites out of the entire dish, restricted to areas above the magnetic “hot spots”. To examine the ability to guide the NGF-MNPs towards specific targets in vivo, we examined two model systems. First, we injected and directed magnetic carriers within the sciatic nerve. Second, we injected the MNPs intravenously and showed a significant accumulation of MNPs in mouse retina while using an external magnet that was placed next to one of the eyes. We propose a novel approach to deliver drugs selectively to injured sites, thus, to promote an effective repair with minimal systemic side effects, overcoming current challenges in regenerative therapeutics. |
| first_indexed | 2024-12-21T18:51:00Z |
| format | Article |
| id | doaj.art-11fcc7641b0046cd86d479e5d25327bc |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-21T18:51:00Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-11fcc7641b0046cd86d479e5d25327bc2022-12-21T18:53:45ZengMDPI AGNanomaterials2079-49912018-09-018970710.3390/nano8090707nano8090707Magnetic Targeting of Growth Factors Using Iron Oxide NanoparticlesMichal Marcus0Alexandra Smith1Ahmad Maswadeh2Ziv Shemesh3Idan Zak4Menachem Motiei5Hadas Schori6Shlomo Margel7Amos Sharoni8Orit Shefi9Faculty of Engineering, Bar Ilan University, Ramat Gan 5290002, IsraelBar Ilan Institute of Nanotechnologies and Advanced Materials, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar Ilan University, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar Ilan University, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar Ilan University, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar Ilan University, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar Ilan University, Ramat Gan 5290002, IsraelBar Ilan Institute of Nanotechnologies and Advanced Materials, Ramat Gan 5290002, IsraelBar Ilan Institute of Nanotechnologies and Advanced Materials, Ramat Gan 5290002, IsraelFaculty of Engineering, Bar Ilan University, Ramat Gan 5290002, IsraelGrowth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed “magnetic targeting”, aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for the nervous tissues. Here, we covalently conjugated nerve growth factor to iron oxide nanoparticles (NGF-MNPs) and used controlled magnetic fields to deliver the NGF–MNP complexes to target sites. In order to actuate the magnetic fields a modular magnetic device was designed and fabricated. PC12 cells that were plated homogenously in culture were differentiated selectively only in targeted sites out of the entire dish, restricted to areas above the magnetic “hot spots”. To examine the ability to guide the NGF-MNPs towards specific targets in vivo, we examined two model systems. First, we injected and directed magnetic carriers within the sciatic nerve. Second, we injected the MNPs intravenously and showed a significant accumulation of MNPs in mouse retina while using an external magnet that was placed next to one of the eyes. We propose a novel approach to deliver drugs selectively to injured sites, thus, to promote an effective repair with minimal systemic side effects, overcoming current challenges in regenerative therapeutics.http://www.mdpi.com/2079-4991/8/9/707nerve growth factormagnetic nanoparticlesneuronal regenerationmagnetic targetingsciatic nerve injury |
| spellingShingle | Michal Marcus Alexandra Smith Ahmad Maswadeh Ziv Shemesh Idan Zak Menachem Motiei Hadas Schori Shlomo Margel Amos Sharoni Orit Shefi Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles Nanomaterials nerve growth factor magnetic nanoparticles neuronal regeneration magnetic targeting sciatic nerve injury |
| title | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
| title_full | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
| title_fullStr | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
| title_full_unstemmed | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
| title_short | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
| title_sort | magnetic targeting of growth factors using iron oxide nanoparticles |
| topic | nerve growth factor magnetic nanoparticles neuronal regeneration magnetic targeting sciatic nerve injury |
| url | http://www.mdpi.com/2079-4991/8/9/707 |
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