Effect of a Third Dose of SARS-CoV-2 mRNA BNT162b2 Vaccine on Humoral and Cellular Responses and Serum Anti-HLA Antibodies in Kidney Transplant Recipients
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has severely impacted on public health, mainly on immunosuppressed patients, including solid organ transplant recipients. Vaccination represents a valuable tool for the prevention of severe SARS-CoV-2 infection, and the immuno...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/6/921 |
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| author | Irene Cassaniti Marilena Gregorini Federica Bergami Francesca Arena Josè Camilla Sammartino Elena Percivalle Ehsan Soleymaninejadian Massimo Abelli Elena Ticozzelli Angela Nocco Francesca Minero Eleonora Francesca Pattonieri Daniele Lilleri Teresa Rampino Fausto Baldanti |
| author_facet | Irene Cassaniti Marilena Gregorini Federica Bergami Francesca Arena Josè Camilla Sammartino Elena Percivalle Ehsan Soleymaninejadian Massimo Abelli Elena Ticozzelli Angela Nocco Francesca Minero Eleonora Francesca Pattonieri Daniele Lilleri Teresa Rampino Fausto Baldanti |
| author_sort | Irene Cassaniti |
| collection | DOAJ |
| description | The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has severely impacted on public health, mainly on immunosuppressed patients, including solid organ transplant recipients. Vaccination represents a valuable tool for the prevention of severe SARS-CoV-2 infection, and the immunogenicity of mRNA vaccines has been evaluated in transplanted patients. In this study, we investigated the role of a third dose of the BNT162b2 vaccine in a cohort of kidney transplant recipients, analyzing both humoral and cell-mediated responses. We observed an increased immune response after the third dose of the vaccine, especially in terms of Spike-specific T cell response. The level of seroconversion remained lower than 50% even after the administration of the third dose. Mycophenolate treatment, steroid administration and age seemed to be associated with a poor immune response. In our cohort, 11/45 patients experienced a SARS-CoV-2 infection after the third vaccine dose. HLA antibodies appearance was recorded in 7 out 45 (15.5%) patients, but none of the patients developed acute renal rejection. Further studies for the evaluation of long-term immune responses are still ongoing, and the impact of a fourth dose of the vaccine will be evaluated. |
| first_indexed | 2024-03-09T22:16:54Z |
| format | Article |
| id | doaj.art-11ff124371b7487f8579e940666b3dc3 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T22:16:54Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-11ff124371b7487f8579e940666b3dc32023-11-23T19:21:27ZengMDPI AGVaccines2076-393X2022-06-0110692110.3390/vaccines10060921Effect of a Third Dose of SARS-CoV-2 mRNA BNT162b2 Vaccine on Humoral and Cellular Responses and Serum Anti-HLA Antibodies in Kidney Transplant RecipientsIrene Cassaniti0Marilena Gregorini1Federica Bergami2Francesca Arena3Josè Camilla Sammartino4Elena Percivalle5Ehsan Soleymaninejadian6Massimo Abelli7Elena Ticozzelli8Angela Nocco9Francesca Minero10Eleonora Francesca Pattonieri11Daniele Lilleri12Teresa Rampino13Fausto Baldanti14Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyDepartment of Internal Medicine and Therapeutics, University of Pavia, 27100 Pavia, ItalyMicrobiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyMicrobiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyMicrobiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyMicrobiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyMicrobiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyTransplant Unit, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyUnit of General Surgery, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyLaboratory of Transplant Immunology, Fondazione IRCCS Ca’ Granda, Ospedale Maggiore Policlinico, 20122 Milan, ItalyUnit of Nephrology, Dialysis, Transplantation, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyUnit of Nephrology, Dialysis, Transplantation, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyMicrobiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyUnit of Nephrology, Dialysis, Transplantation, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyMicrobiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, ItalyThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has severely impacted on public health, mainly on immunosuppressed patients, including solid organ transplant recipients. Vaccination represents a valuable tool for the prevention of severe SARS-CoV-2 infection, and the immunogenicity of mRNA vaccines has been evaluated in transplanted patients. In this study, we investigated the role of a third dose of the BNT162b2 vaccine in a cohort of kidney transplant recipients, analyzing both humoral and cell-mediated responses. We observed an increased immune response after the third dose of the vaccine, especially in terms of Spike-specific T cell response. The level of seroconversion remained lower than 50% even after the administration of the third dose. Mycophenolate treatment, steroid administration and age seemed to be associated with a poor immune response. In our cohort, 11/45 patients experienced a SARS-CoV-2 infection after the third vaccine dose. HLA antibodies appearance was recorded in 7 out 45 (15.5%) patients, but none of the patients developed acute renal rejection. Further studies for the evaluation of long-term immune responses are still ongoing, and the impact of a fourth dose of the vaccine will be evaluated.https://www.mdpi.com/2076-393X/10/6/921transplanted patientsSARS-CoV-2BNT162b2 vaccinethird dosekidneyDSA |
| spellingShingle | Irene Cassaniti Marilena Gregorini Federica Bergami Francesca Arena Josè Camilla Sammartino Elena Percivalle Ehsan Soleymaninejadian Massimo Abelli Elena Ticozzelli Angela Nocco Francesca Minero Eleonora Francesca Pattonieri Daniele Lilleri Teresa Rampino Fausto Baldanti Effect of a Third Dose of SARS-CoV-2 mRNA BNT162b2 Vaccine on Humoral and Cellular Responses and Serum Anti-HLA Antibodies in Kidney Transplant Recipients Vaccines transplanted patients SARS-CoV-2 BNT162b2 vaccine third dose kidney DSA |
| title | Effect of a Third Dose of SARS-CoV-2 mRNA BNT162b2 Vaccine on Humoral and Cellular Responses and Serum Anti-HLA Antibodies in Kidney Transplant Recipients |
| title_full | Effect of a Third Dose of SARS-CoV-2 mRNA BNT162b2 Vaccine on Humoral and Cellular Responses and Serum Anti-HLA Antibodies in Kidney Transplant Recipients |
| title_fullStr | Effect of a Third Dose of SARS-CoV-2 mRNA BNT162b2 Vaccine on Humoral and Cellular Responses and Serum Anti-HLA Antibodies in Kidney Transplant Recipients |
| title_full_unstemmed | Effect of a Third Dose of SARS-CoV-2 mRNA BNT162b2 Vaccine on Humoral and Cellular Responses and Serum Anti-HLA Antibodies in Kidney Transplant Recipients |
| title_short | Effect of a Third Dose of SARS-CoV-2 mRNA BNT162b2 Vaccine on Humoral and Cellular Responses and Serum Anti-HLA Antibodies in Kidney Transplant Recipients |
| title_sort | effect of a third dose of sars cov 2 mrna bnt162b2 vaccine on humoral and cellular responses and serum anti hla antibodies in kidney transplant recipients |
| topic | transplanted patients SARS-CoV-2 BNT162b2 vaccine third dose kidney DSA |
| url | https://www.mdpi.com/2076-393X/10/6/921 |
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