Polo-Like Kinase 4 (PLK4) Is Overexpressed in Central Nervous System Neuroblastoma (CNS-NB)
Neuroblastoma (NB) is the most common extracranial solid tumor in pediatrics, with rare occurrences of primary and metastatic tumors in the central nervous system (CNS). We previously reported the overexpression of the polo-like kinase 4 (PLK4) in embryonal brain tumors. PLK4 has also been found to...
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MDPI AG
2018-11-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/5/4/96 |
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| author | Anders W. Bailey Amreena Suri Pauline M. Chou Tatiana Pundy Samantha Gadd Stacey L. Raimondi Tadanori Tomita Simone Treiger Sredni |
| author_facet | Anders W. Bailey Amreena Suri Pauline M. Chou Tatiana Pundy Samantha Gadd Stacey L. Raimondi Tadanori Tomita Simone Treiger Sredni |
| author_sort | Anders W. Bailey |
| collection | DOAJ |
| description | Neuroblastoma (NB) is the most common extracranial solid tumor in pediatrics, with rare occurrences of primary and metastatic tumors in the central nervous system (CNS). We previously reported the overexpression of the polo-like kinase 4 (PLK4) in embryonal brain tumors. PLK4 has also been found to be overexpressed in a variety of peripheral adult tumors and recently in peripheral NB. Here, we investigated <i>PLK4</i> expression in NBs of the CNS (CNS-NB) and validated our findings by performing a multi-platform transcriptomic meta-analysis using publicly available data. We evaluated the <i>PLK4</i> expression by quantitative real-time PCR (qRT-PCR) on the CNS-NB samples and compared the relative expression levels among other embryonal and non-embryonal brain tumors. The relative <i>PLK4</i> expression levels of the NB samples were found to be significantly higher than the non-embryonal brain tumors (<i>p</i>-value < 0.0001 in both our samples and in public databases). Here, we expand upon our previous work that detected <i>PLK4</i> overexpression in pediatric embryonal tumors to include CNS-NB. As we previously reported, inhibiting PLK4 in embryonal tumors led to decreased tumor cell proliferation, survival, invasion and migration in vitro and tumor growth in vivo, and therefore PLK4 may be a potential new therapeutic approach to CNS-NB. |
| first_indexed | 2024-03-12T19:59:32Z |
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| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T19:59:32Z |
| publishDate | 2018-11-01 |
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| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-11ff6acefb154c15bbb4c8121a6cac3a2023-08-02T02:29:05ZengMDPI AGBioengineering2306-53542018-11-01549610.3390/bioengineering5040096bioengineering5040096Polo-Like Kinase 4 (PLK4) Is Overexpressed in Central Nervous System Neuroblastoma (CNS-NB)Anders W. Bailey0Amreena Suri1Pauline M. Chou2Tatiana Pundy3Samantha Gadd4Stacey L. Raimondi5Tadanori Tomita6Simone Treiger Sredni7Division of Pediatric Neurosurgery, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611, USADivision of Pediatric Neurosurgery, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611, USADepartment of Pathology, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611, USADivision of Pediatric Neurosurgery, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611, USADepartment of Pathology, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611, USADepartment of Biology, Elmhurst College, Elmhurst, IL 60126, USADivision of Pediatric Neurosurgery, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611, USADivision of Pediatric Neurosurgery, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL 60611, USANeuroblastoma (NB) is the most common extracranial solid tumor in pediatrics, with rare occurrences of primary and metastatic tumors in the central nervous system (CNS). We previously reported the overexpression of the polo-like kinase 4 (PLK4) in embryonal brain tumors. PLK4 has also been found to be overexpressed in a variety of peripheral adult tumors and recently in peripheral NB. Here, we investigated <i>PLK4</i> expression in NBs of the CNS (CNS-NB) and validated our findings by performing a multi-platform transcriptomic meta-analysis using publicly available data. We evaluated the <i>PLK4</i> expression by quantitative real-time PCR (qRT-PCR) on the CNS-NB samples and compared the relative expression levels among other embryonal and non-embryonal brain tumors. The relative <i>PLK4</i> expression levels of the NB samples were found to be significantly higher than the non-embryonal brain tumors (<i>p</i>-value < 0.0001 in both our samples and in public databases). Here, we expand upon our previous work that detected <i>PLK4</i> overexpression in pediatric embryonal tumors to include CNS-NB. As we previously reported, inhibiting PLK4 in embryonal tumors led to decreased tumor cell proliferation, survival, invasion and migration in vitro and tumor growth in vivo, and therefore PLK4 may be a potential new therapeutic approach to CNS-NB.https://www.mdpi.com/2306-5354/5/4/96embryonal brain tumorpediatricCNS-PNETlow grade gliomarhabdoidATRTmedulloblastomakinase inhibitor |
| spellingShingle | Anders W. Bailey Amreena Suri Pauline M. Chou Tatiana Pundy Samantha Gadd Stacey L. Raimondi Tadanori Tomita Simone Treiger Sredni Polo-Like Kinase 4 (PLK4) Is Overexpressed in Central Nervous System Neuroblastoma (CNS-NB) Bioengineering embryonal brain tumor pediatric CNS-PNET low grade glioma rhabdoid ATRT medulloblastoma kinase inhibitor |
| title | Polo-Like Kinase 4 (PLK4) Is Overexpressed in Central Nervous System Neuroblastoma (CNS-NB) |
| title_full | Polo-Like Kinase 4 (PLK4) Is Overexpressed in Central Nervous System Neuroblastoma (CNS-NB) |
| title_fullStr | Polo-Like Kinase 4 (PLK4) Is Overexpressed in Central Nervous System Neuroblastoma (CNS-NB) |
| title_full_unstemmed | Polo-Like Kinase 4 (PLK4) Is Overexpressed in Central Nervous System Neuroblastoma (CNS-NB) |
| title_short | Polo-Like Kinase 4 (PLK4) Is Overexpressed in Central Nervous System Neuroblastoma (CNS-NB) |
| title_sort | polo like kinase 4 plk4 is overexpressed in central nervous system neuroblastoma cns nb |
| topic | embryonal brain tumor pediatric CNS-PNET low grade glioma rhabdoid ATRT medulloblastoma kinase inhibitor |
| url | https://www.mdpi.com/2306-5354/5/4/96 |
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