The MSX1 homeoprotein recruits G9a methyltransferase to repressed target genes in myoblast cells.
Although the significance of lysine modifications of core histones for regulating gene expression is widely appreciated, the mechanisms by which these modifications are incorporated at specific regulatory elements during cellular differentiation remains largely unknown. In our previous studies, we h...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3358287?pdf=render |
| _version_ | 1818132657086660608 |
|---|---|
| author | Jingqiang Wang Cory Abate-Shen |
| author_facet | Jingqiang Wang Cory Abate-Shen |
| author_sort | Jingqiang Wang |
| collection | DOAJ |
| description | Although the significance of lysine modifications of core histones for regulating gene expression is widely appreciated, the mechanisms by which these modifications are incorporated at specific regulatory elements during cellular differentiation remains largely unknown. In our previous studies, we have shown that in developing myoblasts the Msx1 homeoprotein represses gene expression by influencing the modification status of chromatin at its target genes. We now show that genomic binding by Msx1 promotes enrichment of the H3K9me2 mark on repressed target genes via recruitment of G9a histone methyltransferase, the enzyme responsible for catalyzing this histone mark. Interaction of Msx1 with G9a is mediated via the homeodomain and is required for transcriptional repression and regulation of cellular differentiation, as well as enrichment of the H3K9me2 mark in proximity to Msx1 binding sites on repressed target genes in myoblast cells as well as the developing limb. We propose that regulation of chromatin status by Msx1 recruitment of G9a and other histone modifying enzymes to regulatory regions of target genes represents an important means of regulating the gene expression during development. |
| first_indexed | 2024-12-11T08:40:18Z |
| format | Article |
| id | doaj.art-120039af380740d0b6913aa359f5efce |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:40:18Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-120039af380740d0b6913aa359f5efce2022-12-22T01:14:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3764710.1371/journal.pone.0037647The MSX1 homeoprotein recruits G9a methyltransferase to repressed target genes in myoblast cells.Jingqiang WangCory Abate-ShenAlthough the significance of lysine modifications of core histones for regulating gene expression is widely appreciated, the mechanisms by which these modifications are incorporated at specific regulatory elements during cellular differentiation remains largely unknown. In our previous studies, we have shown that in developing myoblasts the Msx1 homeoprotein represses gene expression by influencing the modification status of chromatin at its target genes. We now show that genomic binding by Msx1 promotes enrichment of the H3K9me2 mark on repressed target genes via recruitment of G9a histone methyltransferase, the enzyme responsible for catalyzing this histone mark. Interaction of Msx1 with G9a is mediated via the homeodomain and is required for transcriptional repression and regulation of cellular differentiation, as well as enrichment of the H3K9me2 mark in proximity to Msx1 binding sites on repressed target genes in myoblast cells as well as the developing limb. We propose that regulation of chromatin status by Msx1 recruitment of G9a and other histone modifying enzymes to regulatory regions of target genes represents an important means of regulating the gene expression during development.http://europepmc.org/articles/PMC3358287?pdf=render |
| spellingShingle | Jingqiang Wang Cory Abate-Shen The MSX1 homeoprotein recruits G9a methyltransferase to repressed target genes in myoblast cells. PLoS ONE |
| title | The MSX1 homeoprotein recruits G9a methyltransferase to repressed target genes in myoblast cells. |
| title_full | The MSX1 homeoprotein recruits G9a methyltransferase to repressed target genes in myoblast cells. |
| title_fullStr | The MSX1 homeoprotein recruits G9a methyltransferase to repressed target genes in myoblast cells. |
| title_full_unstemmed | The MSX1 homeoprotein recruits G9a methyltransferase to repressed target genes in myoblast cells. |
| title_short | The MSX1 homeoprotein recruits G9a methyltransferase to repressed target genes in myoblast cells. |
| title_sort | msx1 homeoprotein recruits g9a methyltransferase to repressed target genes in myoblast cells |
| url | http://europepmc.org/articles/PMC3358287?pdf=render |
| work_keys_str_mv | AT jingqiangwang themsx1homeoproteinrecruitsg9amethyltransferasetorepressedtargetgenesinmyoblastcells AT coryabateshen themsx1homeoproteinrecruitsg9amethyltransferasetorepressedtargetgenesinmyoblastcells AT jingqiangwang msx1homeoproteinrecruitsg9amethyltransferasetorepressedtargetgenesinmyoblastcells AT coryabateshen msx1homeoproteinrecruitsg9amethyltransferasetorepressedtargetgenesinmyoblastcells |