Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury
Background: Multiple cardiac troponin I (cTnI) immunoassays are commercially available. Overall, assays have not been standardized, and inter-assay differences in the detection of the analyte cardiac troponin I can be clinically relevant.Objective: To compare the diagnostic accuracy of the commercia...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2020.00531/full |
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| author | Joe S. Smith Joe S. Smith Anita Varga Karsten E. Schober |
| author_facet | Joe S. Smith Joe S. Smith Anita Varga Karsten E. Schober |
| author_sort | Joe S. Smith |
| collection | DOAJ |
| description | Background: Multiple cardiac troponin I (cTnI) immunoassays are commercially available. Overall, assays have not been standardized, and inter-assay differences in the detection of the analyte cardiac troponin I can be clinically relevant.Objective: To compare the diagnostic accuracy of the commercially available Abbott i-STAT®1 cTnI immunoassay (i-STAT) and the previously validated ADVIA Centaur TnI-Ultra immunoassay (Centaur) in cattle.Hypothesis: There will be significant differences in bovine serum cTnI results measured by the Centaur and i-STAT methods.Animals: Ten dairy cows with experimentally induced myocardial injury due to monensin administration. Thirty apparently healthy dairy cows with no history of monensin exposure served as controls.Methods: Blood was collected at various time points after administration of a single dose of monensin (20 to 50 mg/kg) via orogastric tube. A total of 112 blood samples were collected. Cardiac TnI concentration was analyzed with the two methods and the association between methods analyzed via linear regression. Bland-Altman analysis to evaluate agreement between methods was performed on samples divided into groups (cTnI < 1.0 ng/mL and cTnI ≥ 1.0 ng/mL).Results: Analyzer results were linearly correlated with each other (R2 = 0.931). Samples with cTnI concentrations <1.0 ng/mL had a bias of −0.13 ± 0.20 ng/mL and samples with cTnI concentrations >1.0 ng/mL had a bias of −9.81 ± 13.26 ng/mL.Conclusions and clinical importance: The results of this study reveal that cTnI concentrations determined with the i-STAT are systematically lower compared to the concentrations determined by the Centaur. |
| first_indexed | 2024-12-21T10:33:30Z |
| format | Article |
| id | doaj.art-12016d3fdbf14c0bbba1c635f4055289 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-21T10:33:30Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-12016d3fdbf14c0bbba1c635f40552892022-12-21T19:07:08ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-08-01710.3389/fvets.2020.00531559031Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial InjuryJoe S. Smith0Joe S. Smith1Anita Varga2Karsten E. Schober3Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesDepartment of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesGold Coast Veterinary Service and Consulting, Esparto, CA, United StatesDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United StatesBackground: Multiple cardiac troponin I (cTnI) immunoassays are commercially available. Overall, assays have not been standardized, and inter-assay differences in the detection of the analyte cardiac troponin I can be clinically relevant.Objective: To compare the diagnostic accuracy of the commercially available Abbott i-STAT®1 cTnI immunoassay (i-STAT) and the previously validated ADVIA Centaur TnI-Ultra immunoassay (Centaur) in cattle.Hypothesis: There will be significant differences in bovine serum cTnI results measured by the Centaur and i-STAT methods.Animals: Ten dairy cows with experimentally induced myocardial injury due to monensin administration. Thirty apparently healthy dairy cows with no history of monensin exposure served as controls.Methods: Blood was collected at various time points after administration of a single dose of monensin (20 to 50 mg/kg) via orogastric tube. A total of 112 blood samples were collected. Cardiac TnI concentration was analyzed with the two methods and the association between methods analyzed via linear regression. Bland-Altman analysis to evaluate agreement between methods was performed on samples divided into groups (cTnI < 1.0 ng/mL and cTnI ≥ 1.0 ng/mL).Results: Analyzer results were linearly correlated with each other (R2 = 0.931). Samples with cTnI concentrations <1.0 ng/mL had a bias of −0.13 ± 0.20 ng/mL and samples with cTnI concentrations >1.0 ng/mL had a bias of −9.81 ± 13.26 ng/mL.Conclusions and clinical importance: The results of this study reveal that cTnI concentrations determined with the i-STAT are systematically lower compared to the concentrations determined by the Centaur.https://www.frontiersin.org/article/10.3389/fvets.2020.00531/fullcardiac troponin Icattleionophorei-STATmonensintoxicity |
| spellingShingle | Joe S. Smith Joe S. Smith Anita Varga Karsten E. Schober Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury Frontiers in Veterinary Science cardiac troponin I cattle ionophore i-STAT monensin toxicity |
| title | Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury |
| title_full | Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury |
| title_fullStr | Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury |
| title_full_unstemmed | Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury |
| title_short | Comparison of Two Commercially Available Immunoassays for the Measurement of Bovine Cardiac Troponin I in Cattle With Induced Myocardial Injury |
| title_sort | comparison of two commercially available immunoassays for the measurement of bovine cardiac troponin i in cattle with induced myocardial injury |
| topic | cardiac troponin I cattle ionophore i-STAT monensin toxicity |
| url | https://www.frontiersin.org/article/10.3389/fvets.2020.00531/full |
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