Mouse models for the study of postnatal cardiac hypertrophy
The main objective of this study was to create a postnatal model for cardiac hypertrophy (CH), in order to explain the mechanisms that are present in childhood cardiac hypertrophy. Five days after implantation, intraperitoneal (IP) isoproterenol (ISO) was injected for 7 days to pregnant female mice....
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | International Journal of Cardiology: Heart & Vasculature |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352906715000202 |
| _version_ | 1818312985120079872 |
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| author | A. Del Olmo-Turrubiarte A. Calzada-Torres G. Díaz-Rosas I. Palma-Lara R. Sánchez-Urbina N.A. Balderrábano-Saucedo H. González-Márquez P. Garcia-Alonso A. Contreras-Ramos |
| author_facet | A. Del Olmo-Turrubiarte A. Calzada-Torres G. Díaz-Rosas I. Palma-Lara R. Sánchez-Urbina N.A. Balderrábano-Saucedo H. González-Márquez P. Garcia-Alonso A. Contreras-Ramos |
| author_sort | A. Del Olmo-Turrubiarte |
| collection | DOAJ |
| description | The main objective of this study was to create a postnatal model for cardiac hypertrophy (CH), in order to explain the mechanisms that are present in childhood cardiac hypertrophy. Five days after implantation, intraperitoneal (IP) isoproterenol (ISO) was injected for 7 days to pregnant female mice. The fetuses were obtained at 15, 17 and 19 dpc from both groups, also newborns (NB), neonates (7–15 days) and young adults (6 weeks of age). Histopathological exams were done on the hearts. Immunohistochemistry and western blot demonstrated GATA4 and PCNA protein expression, qPCR real time the mRNA of adrenergic receptors (α-AR and β-AR), alpha and beta myosins (α-MHC, β-MHC) and GATA4. After the administration of ISO, there was no change in the number of offsprings. We observed significant structural changes in the size of the offspring hearts. Morphometric analysis revealed an increase in the size of the left ventricular wall and interventricular septum (IVS). Histopathological analysis demonstrated loss of cellular compaction and presence of left ventricular small fibrous foci after birth. Adrenergic receptors might be responsible for changing a physiological into a pathological hypertrophy. However GATA4 seemed to be the determining factor in the pathology. A new animal model was established for the study of pathologic CH in early postnatal stages. |
| first_indexed | 2024-12-13T08:26:33Z |
| format | Article |
| id | doaj.art-12018dec1d1c424e845313b9b95c6922 |
| institution | Directory Open Access Journal |
| issn | 2352-9067 |
| language | English |
| last_indexed | 2024-12-13T08:26:33Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Cardiology: Heart & Vasculature |
| spelling | doaj.art-12018dec1d1c424e845313b9b95c69222022-12-21T23:53:52ZengElsevierInternational Journal of Cardiology: Heart & Vasculature2352-90672015-06-017C13114010.1016/j.ijcha.2015.02.005Mouse models for the study of postnatal cardiac hypertrophyA. Del Olmo-Turrubiarte0A. Calzada-Torres1G. Díaz-Rosas2I. Palma-Lara3R. Sánchez-Urbina4N.A. Balderrábano-Saucedo5H. González-Márquez6P. Garcia-Alonso7A. Contreras-Ramos8Laboratorio de Investigación de Biología del Desarrollo y Teratogénesis Experimental, Hospital Infantil de México Federico Gómez (HIMFG), MexicoLaboratorio de Investigación de Biología del Desarrollo y Teratogénesis Experimental, Hospital Infantil de México Federico Gómez (HIMFG), MexicoLaboratorio de Investigación de Biología del Desarrollo y Teratogénesis Experimental, Hospital Infantil de México Federico Gómez (HIMFG), MexicoEscuela Superior de Medicina del IPN, MexicoLaboratorio de Investigación de Biología del Desarrollo y Teratogénesis Experimental, Hospital Infantil de México Federico Gómez (HIMFG), MexicoLaboratorio de Cardiopatías Congénitas, HIMFG, MexicoDepartamento de Ciencias de la Salud, Universidad Autónoma Metropolitana, MexicoInstituto Nacional de Perinatología, MexicoLaboratorio de Investigación de Biología del Desarrollo y Teratogénesis Experimental, Hospital Infantil de México Federico Gómez (HIMFG), MexicoThe main objective of this study was to create a postnatal model for cardiac hypertrophy (CH), in order to explain the mechanisms that are present in childhood cardiac hypertrophy. Five days after implantation, intraperitoneal (IP) isoproterenol (ISO) was injected for 7 days to pregnant female mice. The fetuses were obtained at 15, 17 and 19 dpc from both groups, also newborns (NB), neonates (7–15 days) and young adults (6 weeks of age). Histopathological exams were done on the hearts. Immunohistochemistry and western blot demonstrated GATA4 and PCNA protein expression, qPCR real time the mRNA of adrenergic receptors (α-AR and β-AR), alpha and beta myosins (α-MHC, β-MHC) and GATA4. After the administration of ISO, there was no change in the number of offsprings. We observed significant structural changes in the size of the offspring hearts. Morphometric analysis revealed an increase in the size of the left ventricular wall and interventricular septum (IVS). Histopathological analysis demonstrated loss of cellular compaction and presence of left ventricular small fibrous foci after birth. Adrenergic receptors might be responsible for changing a physiological into a pathological hypertrophy. However GATA4 seemed to be the determining factor in the pathology. A new animal model was established for the study of pathologic CH in early postnatal stages.http://www.sciencedirect.com/science/article/pii/S2352906715000202Fetal cardiac hypertrophyIsoproterenolGATA4α-MHCβ-MHC |
| spellingShingle | A. Del Olmo-Turrubiarte A. Calzada-Torres G. Díaz-Rosas I. Palma-Lara R. Sánchez-Urbina N.A. Balderrábano-Saucedo H. González-Márquez P. Garcia-Alonso A. Contreras-Ramos Mouse models for the study of postnatal cardiac hypertrophy International Journal of Cardiology: Heart & Vasculature Fetal cardiac hypertrophy Isoproterenol GATA4 α-MHC β-MHC |
| title | Mouse models for the study of postnatal cardiac hypertrophy |
| title_full | Mouse models for the study of postnatal cardiac hypertrophy |
| title_fullStr | Mouse models for the study of postnatal cardiac hypertrophy |
| title_full_unstemmed | Mouse models for the study of postnatal cardiac hypertrophy |
| title_short | Mouse models for the study of postnatal cardiac hypertrophy |
| title_sort | mouse models for the study of postnatal cardiac hypertrophy |
| topic | Fetal cardiac hypertrophy Isoproterenol GATA4 α-MHC β-MHC |
| url | http://www.sciencedirect.com/science/article/pii/S2352906715000202 |
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