Microbiological Quality and Antimicrobial Resistance of Commercial Probiotic Products for Food-Producing Animals
Probiotics have been popularly used in livestock production as an alternative to antibiotics. This study aimed to investigate the microbiological quality and phenotypic and genotypic antimicrobial resistance of bacteria in probiotic products sold for food animals. A total of 45 probiotic products we...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/2/148 |
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| author | Hoang My Tran Rangsiya Prathan Si Thu Hein Rungtip Chuanchuen |
| author_facet | Hoang My Tran Rangsiya Prathan Si Thu Hein Rungtip Chuanchuen |
| author_sort | Hoang My Tran |
| collection | DOAJ |
| description | Probiotics have been popularly used in livestock production as an alternative to antibiotics. This study aimed to investigate the microbiological quality and phenotypic and genotypic antimicrobial resistance of bacteria in probiotic products sold for food animals. A total of 45 probiotic products were examined for the number of viable cells, species, and antimicrobial susceptibility; the contamination of <i>Escherichia coli</i> and <i>Salmonella</i>; and the presence of 112 genes encoding resistance to clinically important antimicrobials and transferability of AMR determinants. The results showed that 29 of 45 products (64.4%) were incorrectly labeled in either number of viable cells or bacterial species. None of the tested products were contaminated with <i>E. coli</i> and <i>Salmonella</i>. A total of 33 out of 64 bacterial isolates (51.6%) exhibited resistance to at least one antimicrobial agent. Of the 45 products tested, 16 (35.5%) carried AMR genes. Almost all AMR genes detected in probiotic products were not correlated to the AMR phenotype of probiotic strains formulated in the products. Three streptomycin-resistant <i>Lactobacillus</i> isolates could horizontally transfer their AMR determinants. The findings demonstrated that the probiotic products could serve as reservoirs for the spread of AMR genes and may not yield benefits to animals as claimed. The need for the adequate quality control of probiotic products is highlighted. |
| first_indexed | 2024-03-07T22:44:13Z |
| format | Article |
| id | doaj.art-1203faabb5a54a16a36945c80825f452 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-07T22:44:13Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-1203faabb5a54a16a36945c80825f4522024-02-23T15:05:04ZengMDPI AGAntibiotics2079-63822024-02-0113214810.3390/antibiotics13020148Microbiological Quality and Antimicrobial Resistance of Commercial Probiotic Products for Food-Producing AnimalsHoang My Tran0Rangsiya Prathan1Si Thu Hein2Rungtip Chuanchuen3The International Graduate Course of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandResearch Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandResearch Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandResearch Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandProbiotics have been popularly used in livestock production as an alternative to antibiotics. This study aimed to investigate the microbiological quality and phenotypic and genotypic antimicrobial resistance of bacteria in probiotic products sold for food animals. A total of 45 probiotic products were examined for the number of viable cells, species, and antimicrobial susceptibility; the contamination of <i>Escherichia coli</i> and <i>Salmonella</i>; and the presence of 112 genes encoding resistance to clinically important antimicrobials and transferability of AMR determinants. The results showed that 29 of 45 products (64.4%) were incorrectly labeled in either number of viable cells or bacterial species. None of the tested products were contaminated with <i>E. coli</i> and <i>Salmonella</i>. A total of 33 out of 64 bacterial isolates (51.6%) exhibited resistance to at least one antimicrobial agent. Of the 45 products tested, 16 (35.5%) carried AMR genes. Almost all AMR genes detected in probiotic products were not correlated to the AMR phenotype of probiotic strains formulated in the products. Three streptomycin-resistant <i>Lactobacillus</i> isolates could horizontally transfer their AMR determinants. The findings demonstrated that the probiotic products could serve as reservoirs for the spread of AMR genes and may not yield benefits to animals as claimed. The need for the adequate quality control of probiotic products is highlighted.https://www.mdpi.com/2079-6382/13/2/148antimicrobial resistancefood animalsprobiotics |
| spellingShingle | Hoang My Tran Rangsiya Prathan Si Thu Hein Rungtip Chuanchuen Microbiological Quality and Antimicrobial Resistance of Commercial Probiotic Products for Food-Producing Animals Antibiotics antimicrobial resistance food animals probiotics |
| title | Microbiological Quality and Antimicrobial Resistance of Commercial Probiotic Products for Food-Producing Animals |
| title_full | Microbiological Quality and Antimicrobial Resistance of Commercial Probiotic Products for Food-Producing Animals |
| title_fullStr | Microbiological Quality and Antimicrobial Resistance of Commercial Probiotic Products for Food-Producing Animals |
| title_full_unstemmed | Microbiological Quality and Antimicrobial Resistance of Commercial Probiotic Products for Food-Producing Animals |
| title_short | Microbiological Quality and Antimicrobial Resistance of Commercial Probiotic Products for Food-Producing Animals |
| title_sort | microbiological quality and antimicrobial resistance of commercial probiotic products for food producing animals |
| topic | antimicrobial resistance food animals probiotics |
| url | https://www.mdpi.com/2079-6382/13/2/148 |
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