Inhibitory effects of plant extracts on growth, development and α-amylase activity in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae)
The bioinsecticidal effects of methanolic extracts of the leaves of castor bean, Ricinus communis, and papaya, Carica papaya, on the red flour beetle, Tribolium castaneum, were studied. More specifically, larval weight, larval mortality, percentage pupation, percentage adult emergence and F1 progeny...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2014-05-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-201402-0004_Inhibitory_effects_of_plant_extracts_on_growth_development_and_alpha_-amylase_activity_in_the_red_flour_beetl.php |
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| author | Nujira TATUN Boonguea VAJARASATHIRA Jatuporn TUNGJITWITAYAKUL Sho SAKURAI |
| author_facet | Nujira TATUN Boonguea VAJARASATHIRA Jatuporn TUNGJITWITAYAKUL Sho SAKURAI |
| author_sort | Nujira TATUN |
| collection | DOAJ |
| description | The bioinsecticidal effects of methanolic extracts of the leaves of castor bean, Ricinus communis, and papaya, Carica papaya, on the red flour beetle, Tribolium castaneum, were studied. More specifically, larval weight, larval mortality, percentage pupation, percentage adult emergence and F1 progeny production were recorded. We also studied the effects of the extracts on α-amylase activity and amylase gene expression in T. castaneum. The extracts of R. communis and C. papaya, which were administered in a diet, increased larval mortality and extended the durations of the larval and pupal periods. Feeding them an extract-treated diet also reduced the percentages of larvae that pupated and of adults that emerged and the number of F1 progeny. α-Amylase activity was inhibited and glucose content reduced in the larvae and adults of these insects. This inhibition of α-amylase activity by the R. communis and C. papaya extracts was concentration dependent in vitro. In addition, amylase gene expression was reduced in insects fed a diet containing C. papaya, but not R. communis extract. These results indicate that the bioinsecticidal effects of the leaf extracts are mediated by inhibiting the gene expression and activity of enzymes involved in sugar metabolism. |
| first_indexed | 2024-12-16T14:24:07Z |
| format | Article |
| id | doaj.art-12055193b0a6437195406ff19ed8bf83 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-16T14:24:07Z |
| publishDate | 2014-05-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-12055193b0a6437195406ff19ed8bf832022-12-21T22:28:24ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292014-05-01111218118810.14411/eje.2014.022eje-201402-0004Inhibitory effects of plant extracts on growth, development and α-amylase activity in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae)Nujira TATUN0Boonguea VAJARASATHIRA1Jatuporn TUNGJITWITAYAKUL2Sho SAKURAI3School of Science, University of Phayao, Phayao 56000, Thailand; e-mail: nujira.ta@up.ac.thDepartment of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandSchool of Science, University of Phayao, Phayao 56000, Thailand; e-mail: nujira.ta@up.ac.thDivision of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, JapanThe bioinsecticidal effects of methanolic extracts of the leaves of castor bean, Ricinus communis, and papaya, Carica papaya, on the red flour beetle, Tribolium castaneum, were studied. More specifically, larval weight, larval mortality, percentage pupation, percentage adult emergence and F1 progeny production were recorded. We also studied the effects of the extracts on α-amylase activity and amylase gene expression in T. castaneum. The extracts of R. communis and C. papaya, which were administered in a diet, increased larval mortality and extended the durations of the larval and pupal periods. Feeding them an extract-treated diet also reduced the percentages of larvae that pupated and of adults that emerged and the number of F1 progeny. α-Amylase activity was inhibited and glucose content reduced in the larvae and adults of these insects. This inhibition of α-amylase activity by the R. communis and C. papaya extracts was concentration dependent in vitro. In addition, amylase gene expression was reduced in insects fed a diet containing C. papaya, but not R. communis extract. These results indicate that the bioinsecticidal effects of the leaf extracts are mediated by inhibiting the gene expression and activity of enzymes involved in sugar metabolism.https://www.eje.cz/artkey/eje-201402-0004_Inhibitory_effects_of_plant_extracts_on_growth_development_and_alpha_-amylase_activity_in_the_red_flour_beetl.phpcoleopteratenebrionidaeα-amylaseamylase inhibitorcarica papayaricinus communistribolium castaneum |
| spellingShingle | Nujira TATUN Boonguea VAJARASATHIRA Jatuporn TUNGJITWITAYAKUL Sho SAKURAI Inhibitory effects of plant extracts on growth, development and α-amylase activity in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) European Journal of Entomology coleoptera tenebrionidae α-amylase amylase inhibitor carica papaya ricinus communis tribolium castaneum |
| title | Inhibitory effects of plant extracts on growth, development and α-amylase activity in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_full | Inhibitory effects of plant extracts on growth, development and α-amylase activity in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_fullStr | Inhibitory effects of plant extracts on growth, development and α-amylase activity in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_full_unstemmed | Inhibitory effects of plant extracts on growth, development and α-amylase activity in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_short | Inhibitory effects of plant extracts on growth, development and α-amylase activity in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_sort | inhibitory effects of plant extracts on growth development and α amylase activity in the red flour beetle tribolium castaneum coleoptera tenebrionidae |
| topic | coleoptera tenebrionidae α-amylase amylase inhibitor carica papaya ricinus communis tribolium castaneum |
| url | https://www.eje.cz/artkey/eje-201402-0004_Inhibitory_effects_of_plant_extracts_on_growth_development_and_alpha_-amylase_activity_in_the_red_flour_beetl.php |
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