Immune Responses Regulated by Key Periodontal Bacteria in Germ-Free Mice
The immune dysregulation induced by periodontal bacteria has important roles in the development of periodontitis. However, the role of key periodontal bacteria in local and systemic immunity has not been comprehensively studied. Herein, to explore immunoregulation maps of key periodontal bacteria, a...
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MDPI AG
2022-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/5/513 |
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| author | Xin Shen Yutao Yang Jian Li Bo Zhang Wei Wei Changqing Lu Caixia Yan Hong Wei Yan Li |
| author_facet | Xin Shen Yutao Yang Jian Li Bo Zhang Wei Wei Changqing Lu Caixia Yan Hong Wei Yan Li |
| author_sort | Xin Shen |
| collection | DOAJ |
| description | The immune dysregulation induced by periodontal bacteria has important roles in the development of periodontitis. However, the role of key periodontal bacteria in local and systemic immunity has not been comprehensively studied. Herein, to explore immunoregulation maps of key periodontal bacteria, a mono-colonized germ-free mice model with <i>P. gingivalis</i>, <i>F. nucleatum</i>, and <i>T. denticola</i> for two weeks was designed in this study. The alveolar bone loss was determined by micro-CT. A total of 14 types of innate and adaptive immune cells of the gingiva, spleen, and colon were detected by multi-color flow cytometry. <i>P. gingivalis</i> induced the strongest innate immune response in gingiva and mononuclear phagocytes (MNPs) changed most significantly, compared to <i>F. nucleatum</i> and <i>T. denticola</i>. Immune dysregulation of the colon was widely induced by <i>F. nucleatum</i>. <i>T. denticola</i> mainly induced immune disorder in spleen. ILC3s, Tregs, CD11B+ dendritic cells s, MNPs, macrophages, and plasmacytoid dendritic cells were the main types in response to key periodontal bacteria. However, the alveolar bone loss was not induced by key periodontal bacteria. In conclusion, the overall immunoregulation of monomicrobial stimuli to decipher the complexities of periodontitis was provided in this study. <i>P. gingivalis</i>, <i>F. nucleatum</i>, and <i>T. denticola</i> have different effects on local and systemic immunity in gingiva, colon, and spleen of germ-free mice. |
| first_indexed | 2024-03-10T03:12:04Z |
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| language | English |
| last_indexed | 2024-03-10T03:12:04Z |
| publishDate | 2022-04-01 |
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| series | Pathogens |
| spelling | doaj.art-1206d355dfb04ac59a7f945edbf87ca02023-11-23T12:31:49ZengMDPI AGPathogens2076-08172022-04-0111551310.3390/pathogens11050513Immune Responses Regulated by Key Periodontal Bacteria in Germ-Free MiceXin Shen0Yutao Yang1Jian Li2Bo Zhang3Wei Wei4Changqing Lu5Caixia Yan6Hong Wei7Yan Li8State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaInstitute of Immunology, PLA, Army Medical University, Chongqing 400038, ChinaDepartment of Stomatology, Minda Hospital of Hubei Minzu University, Enshi 445000, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaDepartment of Anatomy, West China School of Basic Medical and Forensic Medicine, Sichuan University, Chengdu 610041, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaCentral Laboratory, Clinical Medicine Scientific and Technical Innovation Park, Shanghai Tenth People’s Hospital, Tongji University, Shanghai 200435, ChinaState Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, ChinaThe immune dysregulation induced by periodontal bacteria has important roles in the development of periodontitis. However, the role of key periodontal bacteria in local and systemic immunity has not been comprehensively studied. Herein, to explore immunoregulation maps of key periodontal bacteria, a mono-colonized germ-free mice model with <i>P. gingivalis</i>, <i>F. nucleatum</i>, and <i>T. denticola</i> for two weeks was designed in this study. The alveolar bone loss was determined by micro-CT. A total of 14 types of innate and adaptive immune cells of the gingiva, spleen, and colon were detected by multi-color flow cytometry. <i>P. gingivalis</i> induced the strongest innate immune response in gingiva and mononuclear phagocytes (MNPs) changed most significantly, compared to <i>F. nucleatum</i> and <i>T. denticola</i>. Immune dysregulation of the colon was widely induced by <i>F. nucleatum</i>. <i>T. denticola</i> mainly induced immune disorder in spleen. ILC3s, Tregs, CD11B+ dendritic cells s, MNPs, macrophages, and plasmacytoid dendritic cells were the main types in response to key periodontal bacteria. However, the alveolar bone loss was not induced by key periodontal bacteria. In conclusion, the overall immunoregulation of monomicrobial stimuli to decipher the complexities of periodontitis was provided in this study. <i>P. gingivalis</i>, <i>F. nucleatum</i>, and <i>T. denticola</i> have different effects on local and systemic immunity in gingiva, colon, and spleen of germ-free mice.https://www.mdpi.com/2076-0817/11/5/513periodontal bacteriainnate immuneadaptive immunegerm-free |
| spellingShingle | Xin Shen Yutao Yang Jian Li Bo Zhang Wei Wei Changqing Lu Caixia Yan Hong Wei Yan Li Immune Responses Regulated by Key Periodontal Bacteria in Germ-Free Mice Pathogens periodontal bacteria innate immune adaptive immune germ-free |
| title | Immune Responses Regulated by Key Periodontal Bacteria in Germ-Free Mice |
| title_full | Immune Responses Regulated by Key Periodontal Bacteria in Germ-Free Mice |
| title_fullStr | Immune Responses Regulated by Key Periodontal Bacteria in Germ-Free Mice |
| title_full_unstemmed | Immune Responses Regulated by Key Periodontal Bacteria in Germ-Free Mice |
| title_short | Immune Responses Regulated by Key Periodontal Bacteria in Germ-Free Mice |
| title_sort | immune responses regulated by key periodontal bacteria in germ free mice |
| topic | periodontal bacteria innate immune adaptive immune germ-free |
| url | https://www.mdpi.com/2076-0817/11/5/513 |
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