Amelioration of oxidative stress, inflammation and liver function by nutraceuticals in rat model of hepatic cancer initiation induced by N-nitrosodiethylamine
Introduction: Nutraceuticals might serve as protective agent against liver cancer induced by pro-cancerous chemicals that initiate high oxidative stress, inflammation and affect DNA integrity. The aim of the present research was to study the prevention of hepatocellular carcinoma initiation induced...
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| Format: | Article |
| Language: | English |
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Shahrekord University of Medical Sciences
2021-04-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | http://herbmedpharmacol.com/PDF/jhp-10-194.pdf |
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| author | Sahar Y. Al-Okbi Doha A. Mohamed Hagar F.H. Elbakry |
| author_facet | Sahar Y. Al-Okbi Doha A. Mohamed Hagar F.H. Elbakry |
| author_sort | Sahar Y. Al-Okbi |
| collection | DOAJ |
| description | Introduction: Nutraceuticals might serve as protective agent against liver cancer induced by pro-cancerous chemicals that initiate high oxidative stress, inflammation and affect DNA integrity. The aim of the present research was to study the prevention of hepatocellular carcinoma initiation induced by N-nitrosodiethylamine (NDEA) through treatment by nutraceuticals. Methods: Two nutraceuticals were prepared; the first (NI) was a mixture of different extracts of green tea, wheat germ and tomato, the second one (NII) was composed of extracts mixture of broccoli, hazelnuts and carrot. Total flavonoids and flavonols were determined in the nutraceuticals. Four groups of rats were run; the first served as control normal, the other three groups were treated by intraperitoneal injection of NDEA, one of these groups was designated as control NDEA, the other two groups (test groups) were treated daily with oral doses of NI and NII, respectively. The experiment continued for 8 weeks. Plasma transaminases, alkaline phosphatase and catalase activities, total protein, albumin, malondialdehyde (MDA) and tumor necrosis factor-α (TNF-α) along with liver MDA level and catalase activity were assessed. Results: NI showed higher flavonoids and lower flavonols than NII (P < 0.05). High oxidative stress and inflammation biomarkers, liver dysfunction, reduced plasma albumin and total protein were demonstrated in control NDEA compared to control normal (P < 0.05). Test groups showed significant improvement in all parameters (P < 0.05) compared to NDEA control. NI was superior in improving plasma transaminases and catalase activities, MDA and TNF-α levels and liver catalase activity compared to NII (P < 0.05). Conclusion: Both NI and NII might prevent liver cancer initiation during exposure to carcinogenic agents, NI being superior to NII. |
| first_indexed | 2024-12-10T17:29:28Z |
| format | Article |
| id | doaj.art-1208060b90d84359bf182886d9078945 |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-12-10T17:29:28Z |
| publishDate | 2021-04-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-1208060b90d84359bf182886d90789452022-12-22T01:39:44ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042021-04-0110219420110.34172/jhp.2021.21jhp-36648Amelioration of oxidative stress, inflammation and liver function by nutraceuticals in rat model of hepatic cancer initiation induced by N-nitrosodiethylamineSahar Y. Al-Okbi0Doha A. Mohamed1Hagar F.H. Elbakry2Nutrition and Food Sciences Department, National Research Centre, Cairo, EgyptNutrition and Food Sciences Department, National Research Centre, Cairo, EgyptNutrition and Food Sciences Department, National Research Centre, Cairo, EgyptIntroduction: Nutraceuticals might serve as protective agent against liver cancer induced by pro-cancerous chemicals that initiate high oxidative stress, inflammation and affect DNA integrity. The aim of the present research was to study the prevention of hepatocellular carcinoma initiation induced by N-nitrosodiethylamine (NDEA) through treatment by nutraceuticals. Methods: Two nutraceuticals were prepared; the first (NI) was a mixture of different extracts of green tea, wheat germ and tomato, the second one (NII) was composed of extracts mixture of broccoli, hazelnuts and carrot. Total flavonoids and flavonols were determined in the nutraceuticals. Four groups of rats were run; the first served as control normal, the other three groups were treated by intraperitoneal injection of NDEA, one of these groups was designated as control NDEA, the other two groups (test groups) were treated daily with oral doses of NI and NII, respectively. The experiment continued for 8 weeks. Plasma transaminases, alkaline phosphatase and catalase activities, total protein, albumin, malondialdehyde (MDA) and tumor necrosis factor-α (TNF-α) along with liver MDA level and catalase activity were assessed. Results: NI showed higher flavonoids and lower flavonols than NII (P < 0.05). High oxidative stress and inflammation biomarkers, liver dysfunction, reduced plasma albumin and total protein were demonstrated in control NDEA compared to control normal (P < 0.05). Test groups showed significant improvement in all parameters (P < 0.05) compared to NDEA control. NI was superior in improving plasma transaminases and catalase activities, MDA and TNF-α levels and liver catalase activity compared to NII (P < 0.05). Conclusion: Both NI and NII might prevent liver cancer initiation during exposure to carcinogenic agents, NI being superior to NII.http://herbmedpharmacol.com/PDF/jhp-10-194.pdfcancer initiationn-nitrosodiethylamineplants extractsinflammationoxidative stressliver function |
| spellingShingle | Sahar Y. Al-Okbi Doha A. Mohamed Hagar F.H. Elbakry Amelioration of oxidative stress, inflammation and liver function by nutraceuticals in rat model of hepatic cancer initiation induced by N-nitrosodiethylamine Journal of HerbMed Pharmacology cancer initiation n-nitrosodiethylamine plants extracts inflammation oxidative stress liver function |
| title | Amelioration of oxidative stress, inflammation and liver function by nutraceuticals in rat model of hepatic cancer initiation induced by N-nitrosodiethylamine |
| title_full | Amelioration of oxidative stress, inflammation and liver function by nutraceuticals in rat model of hepatic cancer initiation induced by N-nitrosodiethylamine |
| title_fullStr | Amelioration of oxidative stress, inflammation and liver function by nutraceuticals in rat model of hepatic cancer initiation induced by N-nitrosodiethylamine |
| title_full_unstemmed | Amelioration of oxidative stress, inflammation and liver function by nutraceuticals in rat model of hepatic cancer initiation induced by N-nitrosodiethylamine |
| title_short | Amelioration of oxidative stress, inflammation and liver function by nutraceuticals in rat model of hepatic cancer initiation induced by N-nitrosodiethylamine |
| title_sort | amelioration of oxidative stress inflammation and liver function by nutraceuticals in rat model of hepatic cancer initiation induced by n nitrosodiethylamine |
| topic | cancer initiation n-nitrosodiethylamine plants extracts inflammation oxidative stress liver function |
| url | http://herbmedpharmacol.com/PDF/jhp-10-194.pdf |
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