Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)
Abstract Dysregulation of VEGF-A bioavailability has been implicated in the development of lung injury/fibrosis, exemplified by Idiopathic Pulmonary Fibrosis (IPF). VEGF-A is a target of the hypoxic response via its translational regulation by HIF-1α. The role of hypoxia and hyperoxia in the develop...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-01-01
|
| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12931-017-0711-x |
| _version_ | 1818200631896178688 |
|---|---|
| author | Shaney L. Barratt Thomas Blythe Khadija Ourradi Caroline Jarrett Gavin I. Welsh David O. Bates Ann B. Millar |
| author_facet | Shaney L. Barratt Thomas Blythe Khadija Ourradi Caroline Jarrett Gavin I. Welsh David O. Bates Ann B. Millar |
| author_sort | Shaney L. Barratt |
| collection | DOAJ |
| description | Abstract Dysregulation of VEGF-A bioavailability has been implicated in the development of lung injury/fibrosis, exemplified by Idiopathic Pulmonary Fibrosis (IPF). VEGF-A is a target of the hypoxic response via its translational regulation by HIF-1α. The role of hypoxia and hyperoxia in the development and progression of IPF has not been explored. In normal lung (NF) and IPF-derived fibroblasts (FF) VEGF-Axxxa protein expression was upregulated by hypoxia, mediated through activation of VEGF-Axxxa gene transcription. VEGF-A receptors and co-receptors were differentially expressed by hypoxia and hyperoxia. Our data supports a potential role for hypoxia, hyperoxia and VEGF-Axxxa isoforms as drivers of fibrogenesis. |
| first_indexed | 2024-12-12T02:40:44Z |
| format | Article |
| id | doaj.art-120bb84e425d477797fb1bb9fb2175c7 |
| institution | Directory Open Access Journal |
| issn | 1465-993X |
| language | English |
| last_indexed | 2024-12-12T02:40:44Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-120bb84e425d477797fb1bb9fb2175c72022-12-22T00:41:09ZengBMCRespiratory Research1465-993X2018-01-011911510.1186/s12931-017-0711-xEffects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)Shaney L. Barratt0Thomas Blythe1Khadija Ourradi2Caroline Jarrett3Gavin I. Welsh4David O. Bates5Ann B. Millar6Academic Respiratory Unit, Learning and Research Building, Southmead HospitalAcademic Respiratory Unit, Learning and Research Building, Southmead HospitalAcademic Respiratory Unit, Learning and Research Building, Southmead HospitalAcademic Respiratory Unit, Learning and Research Building, Southmead HospitalBristol Renal, Translational Health Sciences, Bristol Medical School, University of BristolCancer Biology, Division of Cancer and Stem Cells, School of Medicine, University of NottinghamAcademic Respiratory Unit, Learning and Research Building, Southmead HospitalAbstract Dysregulation of VEGF-A bioavailability has been implicated in the development of lung injury/fibrosis, exemplified by Idiopathic Pulmonary Fibrosis (IPF). VEGF-A is a target of the hypoxic response via its translational regulation by HIF-1α. The role of hypoxia and hyperoxia in the development and progression of IPF has not been explored. In normal lung (NF) and IPF-derived fibroblasts (FF) VEGF-Axxxa protein expression was upregulated by hypoxia, mediated through activation of VEGF-Axxxa gene transcription. VEGF-A receptors and co-receptors were differentially expressed by hypoxia and hyperoxia. Our data supports a potential role for hypoxia, hyperoxia and VEGF-Axxxa isoforms as drivers of fibrogenesis.http://link.springer.com/article/10.1186/s12931-017-0711-xInterstitial lung diseaseVascular endothelial growth factorHypoxiaIdiopathic pulmonary fibrosis |
| spellingShingle | Shaney L. Barratt Thomas Blythe Khadija Ourradi Caroline Jarrett Gavin I. Welsh David O. Bates Ann B. Millar Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF) Respiratory Research Interstitial lung disease Vascular endothelial growth factor Hypoxia Idiopathic pulmonary fibrosis |
| title | Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF) |
| title_full | Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF) |
| title_fullStr | Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF) |
| title_full_unstemmed | Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF) |
| title_short | Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF) |
| title_sort | effects of hypoxia and hyperoxia on the differential expression of vegf a isoforms and receptors in idiopathic pulmonary fibrosis ipf |
| topic | Interstitial lung disease Vascular endothelial growth factor Hypoxia Idiopathic pulmonary fibrosis |
| url | http://link.springer.com/article/10.1186/s12931-017-0711-x |
| work_keys_str_mv | AT shaneylbarratt effectsofhypoxiaandhyperoxiaonthedifferentialexpressionofvegfaisoformsandreceptorsinidiopathicpulmonaryfibrosisipf AT thomasblythe effectsofhypoxiaandhyperoxiaonthedifferentialexpressionofvegfaisoformsandreceptorsinidiopathicpulmonaryfibrosisipf AT khadijaourradi effectsofhypoxiaandhyperoxiaonthedifferentialexpressionofvegfaisoformsandreceptorsinidiopathicpulmonaryfibrosisipf AT carolinejarrett effectsofhypoxiaandhyperoxiaonthedifferentialexpressionofvegfaisoformsandreceptorsinidiopathicpulmonaryfibrosisipf AT gaviniwelsh effectsofhypoxiaandhyperoxiaonthedifferentialexpressionofvegfaisoformsandreceptorsinidiopathicpulmonaryfibrosisipf AT davidobates effectsofhypoxiaandhyperoxiaonthedifferentialexpressionofvegfaisoformsandreceptorsinidiopathicpulmonaryfibrosisipf AT annbmillar effectsofhypoxiaandhyperoxiaonthedifferentialexpressionofvegfaisoformsandreceptorsinidiopathicpulmonaryfibrosisipf |