Identification of the Potential Genes Regulating Seed Germination Speed in Maize
Seed germination is the crucial stage in plant life cycle. Rapid and uniform germination plays an essential role in plant development and grain yield improvement. However, the molecular mechanism underlying seed germination speed is largely unknown due to the complexity of the dynamic process and th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/4/556 |
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| author | Huairen Zhang Jie Zang Yanqing Huo Zhaogui Zhang Huabang Chen Xunji Chen Juan Liu |
| author_facet | Huairen Zhang Jie Zang Yanqing Huo Zhaogui Zhang Huabang Chen Xunji Chen Juan Liu |
| author_sort | Huairen Zhang |
| collection | DOAJ |
| description | Seed germination is the crucial stage in plant life cycle. Rapid and uniform germination plays an essential role in plant development and grain yield improvement. However, the molecular mechanism underlying seed germination speed is largely unknown due to the complexity of the dynamic process and the difficulty in phenotyping. Here, we conducted a time-series comparative transcriptome study of two elite maize inbred lines, 72-3 and F9721, with striking difference in seed germination speed, and identified a major locus underlying maize germination speed through genome-wide association analysis (GWAS) of an F<sub>2</sub> segregation population. Comparative transcriptome study identified 12 h after imbibition (HAI) as the critical stage responsible for the variation in germination speed. The differentially expressed genes (DEGs) between 72-3 and F9721 were mainly enriched in metabolic pathways, biosynthesis of secondary metabolites, oxidoreductase activity pathways, hormone signal transduction, and amino acid transporter activity pathways. GWAS revealed that germination speed was controlled by a major locus on chromosome 1 with the leading SNP as AX-91332814, explaining 10.63% of phenotypic variation. A total of 87 proposed protein-coding genes surrounding the locus were integrated with DEGs. Combined with evidence from the gene expression database and gene synteny with other model species, we finally anchored three genes as the likely candidates regulating germination speed in maize. This study provides clues for the further exploration of genes controlling the maize seed germination speed, thus facilitating breeding of rapid germinated elite lines through marker assistant selection. |
| first_indexed | 2024-03-09T21:11:42Z |
| format | Article |
| id | doaj.art-120c499a287847d998aca1811a23662d |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:11:42Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-120c499a287847d998aca1811a23662d2023-11-23T21:43:17ZengMDPI AGPlants2223-77472022-02-0111455610.3390/plants11040556Identification of the Potential Genes Regulating Seed Germination Speed in MaizeHuairen Zhang0Jie Zang1Yanqing Huo2Zhaogui Zhang3Huabang Chen4Xunji Chen5Juan Liu6State Key Laboratory of Plant Cell and Chromosome Engineering, Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Biotech & Nuclear, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaState Key Laboratory of Plant Cell and Chromosome Engineering, Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, ChinaSeed germination is the crucial stage in plant life cycle. Rapid and uniform germination plays an essential role in plant development and grain yield improvement. However, the molecular mechanism underlying seed germination speed is largely unknown due to the complexity of the dynamic process and the difficulty in phenotyping. Here, we conducted a time-series comparative transcriptome study of two elite maize inbred lines, 72-3 and F9721, with striking difference in seed germination speed, and identified a major locus underlying maize germination speed through genome-wide association analysis (GWAS) of an F<sub>2</sub> segregation population. Comparative transcriptome study identified 12 h after imbibition (HAI) as the critical stage responsible for the variation in germination speed. The differentially expressed genes (DEGs) between 72-3 and F9721 were mainly enriched in metabolic pathways, biosynthesis of secondary metabolites, oxidoreductase activity pathways, hormone signal transduction, and amino acid transporter activity pathways. GWAS revealed that germination speed was controlled by a major locus on chromosome 1 with the leading SNP as AX-91332814, explaining 10.63% of phenotypic variation. A total of 87 proposed protein-coding genes surrounding the locus were integrated with DEGs. Combined with evidence from the gene expression database and gene synteny with other model species, we finally anchored three genes as the likely candidates regulating germination speed in maize. This study provides clues for the further exploration of genes controlling the maize seed germination speed, thus facilitating breeding of rapid germinated elite lines through marker assistant selection.https://www.mdpi.com/2223-7747/11/4/556maizeseed germinationdivergent germination speedtime-series transcriptomeGWAS mapping |
| spellingShingle | Huairen Zhang Jie Zang Yanqing Huo Zhaogui Zhang Huabang Chen Xunji Chen Juan Liu Identification of the Potential Genes Regulating Seed Germination Speed in Maize Plants maize seed germination divergent germination speed time-series transcriptome GWAS mapping |
| title | Identification of the Potential Genes Regulating Seed Germination Speed in Maize |
| title_full | Identification of the Potential Genes Regulating Seed Germination Speed in Maize |
| title_fullStr | Identification of the Potential Genes Regulating Seed Germination Speed in Maize |
| title_full_unstemmed | Identification of the Potential Genes Regulating Seed Germination Speed in Maize |
| title_short | Identification of the Potential Genes Regulating Seed Germination Speed in Maize |
| title_sort | identification of the potential genes regulating seed germination speed in maize |
| topic | maize seed germination divergent germination speed time-series transcriptome GWAS mapping |
| url | https://www.mdpi.com/2223-7747/11/4/556 |
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