Metabolic engineering of <it>Saccharomyces cerevisiae </it>for the production of n-butanol
<p>Abstract</p> <p>Background</p> <p>Increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol.</p> <p>Results and conclusion&l...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-12-01
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| Series: | Microbial Cell Factories |
| Online Access: | http://www.microbialcellfactories.com/content/7/1/36 |
| _version_ | 1819035128660230144 |
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| author | Myers Samuel Prasad Nilu Chan Rossana Steen Eric J Petzold Christopher J Redding Alyssa Ouellet Mario Keasling Jay D |
| author_facet | Myers Samuel Prasad Nilu Chan Rossana Steen Eric J Petzold Christopher J Redding Alyssa Ouellet Mario Keasling Jay D |
| author_sort | Myers Samuel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol.</p> <p>Results and conclusion</p> <p><it>Saccharomyces cerevisiae </it>was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (<it>S. cerevisiae</it>, <it>Escherichia coli</it>, <it>Clostridium beijerinckii</it>, and <it>Ralstonia eutropha</it>) were substituted for the Clostridial enzymes and their effect on n-butanol production was compared. By choosing the appropriate isozymes, we were able to improve production of n-butanol ten-fold to 2.5 mg/L. The most productive strains harbored the <it>C. beijerinckii </it>3-hydroxybutyryl-CoA dehydrogenase, which uses NADH as a co-factor, rather than the <it>R. eutropha </it>isozyme, which uses NADPH, and the acetoacetyl-CoA transferase from <it>S. cerevisiae </it>or <it>E. coli </it>rather than that from <it>R. eutropha</it>. Surprisingly, expression of the genes encoding the butyryl-CoA dehydrogenase from <it>C. beijerinckii </it>(<it>bcd </it>and <it>etfAB</it>) did not improve butanol production significantly as previously reported in <it>E. coli</it>. Using metabolite analysis, we were able to determine which steps in the n-butanol biosynthetic pathway were the most problematic and ripe for future improvement.</p> |
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| format | Article |
| id | doaj.art-120cae3b76c740d8b14229ace9c86fd5 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-21T07:44:42Z |
| publishDate | 2008-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-120cae3b76c740d8b14229ace9c86fd52022-12-21T19:11:13ZengBMCMicrobial Cell Factories1475-28592008-12-01713610.1186/1475-2859-7-36Metabolic engineering of <it>Saccharomyces cerevisiae </it>for the production of n-butanolMyers SamuelPrasad NiluChan RossanaSteen Eric JPetzold Christopher JRedding AlyssaOuellet MarioKeasling Jay D<p>Abstract</p> <p>Background</p> <p>Increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol.</p> <p>Results and conclusion</p> <p><it>Saccharomyces cerevisiae </it>was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (<it>S. cerevisiae</it>, <it>Escherichia coli</it>, <it>Clostridium beijerinckii</it>, and <it>Ralstonia eutropha</it>) were substituted for the Clostridial enzymes and their effect on n-butanol production was compared. By choosing the appropriate isozymes, we were able to improve production of n-butanol ten-fold to 2.5 mg/L. The most productive strains harbored the <it>C. beijerinckii </it>3-hydroxybutyryl-CoA dehydrogenase, which uses NADH as a co-factor, rather than the <it>R. eutropha </it>isozyme, which uses NADPH, and the acetoacetyl-CoA transferase from <it>S. cerevisiae </it>or <it>E. coli </it>rather than that from <it>R. eutropha</it>. Surprisingly, expression of the genes encoding the butyryl-CoA dehydrogenase from <it>C. beijerinckii </it>(<it>bcd </it>and <it>etfAB</it>) did not improve butanol production significantly as previously reported in <it>E. coli</it>. Using metabolite analysis, we were able to determine which steps in the n-butanol biosynthetic pathway were the most problematic and ripe for future improvement.</p>http://www.microbialcellfactories.com/content/7/1/36 |
| spellingShingle | Myers Samuel Prasad Nilu Chan Rossana Steen Eric J Petzold Christopher J Redding Alyssa Ouellet Mario Keasling Jay D Metabolic engineering of <it>Saccharomyces cerevisiae </it>for the production of n-butanol Microbial Cell Factories |
| title | Metabolic engineering of <it>Saccharomyces cerevisiae </it>for the production of n-butanol |
| title_full | Metabolic engineering of <it>Saccharomyces cerevisiae </it>for the production of n-butanol |
| title_fullStr | Metabolic engineering of <it>Saccharomyces cerevisiae </it>for the production of n-butanol |
| title_full_unstemmed | Metabolic engineering of <it>Saccharomyces cerevisiae </it>for the production of n-butanol |
| title_short | Metabolic engineering of <it>Saccharomyces cerevisiae </it>for the production of n-butanol |
| title_sort | metabolic engineering of it saccharomyces cerevisiae it for the production of n butanol |
| url | http://www.microbialcellfactories.com/content/7/1/36 |
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