De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (<i>Trichosurus vulpecula</i>) in New Zealand: Transcriptome Diversity after Decades of Population Control
The common brushtail possum (<i>Trichosurus vulpecula</i>), introduced from Australia in the mid-nineteenth century, is an invasive species in New Zealand where it is widespread and forms the largest self-sustained reservoir of bovine tuberculosis (<i>Mycobacterium bovis</i>)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/4/436 |
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| author | Arsalan Emami-Khoyi Shilpa Pradeep Parbhu James G. Ross Elaine C. Murphy Jennifer Bothwell Daniela M. Monsanto Bettine Jansen van Vuuren Peter R. Teske Adrian M. Paterson |
| author_facet | Arsalan Emami-Khoyi Shilpa Pradeep Parbhu James G. Ross Elaine C. Murphy Jennifer Bothwell Daniela M. Monsanto Bettine Jansen van Vuuren Peter R. Teske Adrian M. Paterson |
| author_sort | Arsalan Emami-Khoyi |
| collection | DOAJ |
| description | The common brushtail possum (<i>Trichosurus vulpecula</i>), introduced from Australia in the mid-nineteenth century, is an invasive species in New Zealand where it is widespread and forms the largest self-sustained reservoir of bovine tuberculosis (<i>Mycobacterium bovis</i>) among wild populations. Conservation and agricultural authorities regularly apply a series of population control measures to suppress brushtail possum populations. The evolutionary consequence of more than half a century of intensive population control operations on the species’ genomic diversity and population structure is hindered by a paucity of available genomic resources. This study is the first to characterise the functional content and diversity of brushtail possum liver and brain cerebral cortex transcriptomes. Raw sequences from hepatic cells and cerebral cortex were assembled into 58,001 and 64,735 transcripts respectively. Functional annotation and polymorphism assignment of the assembled transcripts demonstrated a considerable level of variation in the core metabolic pathways that represent potential targets for selection pressure exerted by chemical toxicants. This study suggests that the brushtail possum population in New Zealand harbours considerable variation in metabolic pathways that could potentially promote the development of tolerance against chemical toxicants. |
| first_indexed | 2024-03-10T20:23:51Z |
| format | Article |
| id | doaj.art-120d3532794d4ddeb9d6a283908071cc |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T20:23:51Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-120d3532794d4ddeb9d6a283908071cc2023-11-19T21:55:15ZengMDPI AGGenes2073-44252020-04-0111443610.3390/genes11040436De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (<i>Trichosurus vulpecula</i>) in New Zealand: Transcriptome Diversity after Decades of Population ControlArsalan Emami-Khoyi0Shilpa Pradeep Parbhu1James G. Ross2Elaine C. Murphy3Jennifer Bothwell4Daniela M. Monsanto5Bettine Jansen van Vuuren6Peter R. Teske7Adrian M. Paterson8Center for Ecological Genomics and Wildlife Conservation, University of Johannesburg, Auckland Park 2006, South AfricaCenter for Ecological Genomics and Wildlife Conservation, University of Johannesburg, Auckland Park 2006, South AfricaDepartment of Pest-management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New ZealandDepartment of Pest-management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New ZealandDepartment of Pest-management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New ZealandCenter for Ecological Genomics and Wildlife Conservation, University of Johannesburg, Auckland Park 2006, South AfricaCenter for Ecological Genomics and Wildlife Conservation, University of Johannesburg, Auckland Park 2006, South AfricaCenter for Ecological Genomics and Wildlife Conservation, University of Johannesburg, Auckland Park 2006, South AfricaDepartment of Pest-management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New ZealandThe common brushtail possum (<i>Trichosurus vulpecula</i>), introduced from Australia in the mid-nineteenth century, is an invasive species in New Zealand where it is widespread and forms the largest self-sustained reservoir of bovine tuberculosis (<i>Mycobacterium bovis</i>) among wild populations. Conservation and agricultural authorities regularly apply a series of population control measures to suppress brushtail possum populations. The evolutionary consequence of more than half a century of intensive population control operations on the species’ genomic diversity and population structure is hindered by a paucity of available genomic resources. This study is the first to characterise the functional content and diversity of brushtail possum liver and brain cerebral cortex transcriptomes. Raw sequences from hepatic cells and cerebral cortex were assembled into 58,001 and 64,735 transcripts respectively. Functional annotation and polymorphism assignment of the assembled transcripts demonstrated a considerable level of variation in the core metabolic pathways that represent potential targets for selection pressure exerted by chemical toxicants. This study suggests that the brushtail possum population in New Zealand harbours considerable variation in metabolic pathways that could potentially promote the development of tolerance against chemical toxicants.https://www.mdpi.com/2073-4425/11/4/436<i>De novo</i> transcriptome assemblycommon brushtail possumliverbrain cerebral cortexdrug and xenobiotic metabolismchemical toxicants |
| spellingShingle | Arsalan Emami-Khoyi Shilpa Pradeep Parbhu James G. Ross Elaine C. Murphy Jennifer Bothwell Daniela M. Monsanto Bettine Jansen van Vuuren Peter R. Teske Adrian M. Paterson De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (<i>Trichosurus vulpecula</i>) in New Zealand: Transcriptome Diversity after Decades of Population Control Genes <i>De novo</i> transcriptome assembly common brushtail possum liver brain cerebral cortex drug and xenobiotic metabolism chemical toxicants |
| title | De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (<i>Trichosurus vulpecula</i>) in New Zealand: Transcriptome Diversity after Decades of Population Control |
| title_full | De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (<i>Trichosurus vulpecula</i>) in New Zealand: Transcriptome Diversity after Decades of Population Control |
| title_fullStr | De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (<i>Trichosurus vulpecula</i>) in New Zealand: Transcriptome Diversity after Decades of Population Control |
| title_full_unstemmed | De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (<i>Trichosurus vulpecula</i>) in New Zealand: Transcriptome Diversity after Decades of Population Control |
| title_short | De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (<i>Trichosurus vulpecula</i>) in New Zealand: Transcriptome Diversity after Decades of Population Control |
| title_sort | de novo transcriptome assembly and annotation of liver and brain tissues of common brushtail possums i trichosurus vulpecula i in new zealand transcriptome diversity after decades of population control |
| topic | <i>De novo</i> transcriptome assembly common brushtail possum liver brain cerebral cortex drug and xenobiotic metabolism chemical toxicants |
| url | https://www.mdpi.com/2073-4425/11/4/436 |
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