<b>Extraction, physical-chemical characterization and <i>in vitro</i> inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds
The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2018-03-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463 |
| _version_ | 1818233753803161600 |
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| author | José Ariévilo Gurgel Rodrigues Priscila Gomes Barcellos Bianca Fernandes Glauser Ana Luíza Gomes Quinderé Norma Maria Barros Benevides Ana Maria Freire Tovar Paulo Antônio de Souza Mourão |
| author_facet | José Ariévilo Gurgel Rodrigues Priscila Gomes Barcellos Bianca Fernandes Glauser Ana Luíza Gomes Quinderé Norma Maria Barros Benevides Ana Maria Freire Tovar Paulo Antônio de Souza Mourão |
| author_sort | José Ariévilo Gurgel Rodrigues |
| collection | DOAJ |
| description |
The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p < 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose electrophoresis, while polyacrylamide analysis revealed SPs of > 100 kDa. These procedures, combined with the use of Stains-All, also indicated nonSPs. APTTs ranged from 2.81 (A. muscoides) to 21.30 IU (Halymenia sp.) vs. heparin (193 IU), and were dependent on sulfation of the crude SPs. PT was not altered. With respect to TG assay, crude SPs modified concentration-dependent and independently from molecular mass TG by both intrinsic/extrinsic pathways in 60-fold diluted human plasma, with total intrinsic inactivation using crude SPs from A. muscoides in parallel to heparin (p < 0.05). Thrombosis in vitro is differentially modulated by distinct crude SPs from Brazilian seaweeds.
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| first_indexed | 2024-12-12T11:27:12Z |
| format | Article |
| id | doaj.art-121129f44f4d42afa27d638982f56794 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-12-12T11:27:12Z |
| publishDate | 2018-03-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-121129f44f4d42afa27d638982f567942022-12-22T00:25:53ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2018-03-0140110.4025/actascibiolsci.v40i1.3446317708<b>Extraction, physical-chemical characterization and <i>in vitro</i> inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweedsJosé Ariévilo Gurgel Rodrigues0Priscila Gomes Barcellos1Bianca Fernandes Glauser2Ana Luíza Gomes Quinderé3Norma Maria Barros Benevides4Ana Maria Freire Tovar5Paulo Antônio de Souza Mourão6Universidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do CearáUniversidade Federal do CearáUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de Janeiro The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p < 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose electrophoresis, while polyacrylamide analysis revealed SPs of > 100 kDa. These procedures, combined with the use of Stains-All, also indicated nonSPs. APTTs ranged from 2.81 (A. muscoides) to 21.30 IU (Halymenia sp.) vs. heparin (193 IU), and were dependent on sulfation of the crude SPs. PT was not altered. With respect to TG assay, crude SPs modified concentration-dependent and independently from molecular mass TG by both intrinsic/extrinsic pathways in 60-fold diluted human plasma, with total intrinsic inactivation using crude SPs from A. muscoides in parallel to heparin (p < 0.05). Thrombosis in vitro is differentially modulated by distinct crude SPs from Brazilian seaweeds. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463marine algaepolyglycanscoagulation teststhrombin. |
| spellingShingle | José Ariévilo Gurgel Rodrigues Priscila Gomes Barcellos Bianca Fernandes Glauser Ana Luíza Gomes Quinderé Norma Maria Barros Benevides Ana Maria Freire Tovar Paulo Antônio de Souza Mourão <b>Extraction, physical-chemical characterization and <i>in vitro</i> inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds Acta Scientiarum: Biological Sciences marine algae polyglycans coagulation tests thrombin. |
| title | <b>Extraction, physical-chemical characterization and <i>in vitro</i> inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
| title_full | <b>Extraction, physical-chemical characterization and <i>in vitro</i> inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
| title_fullStr | <b>Extraction, physical-chemical characterization and <i>in vitro</i> inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
| title_full_unstemmed | <b>Extraction, physical-chemical characterization and <i>in vitro</i> inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
| title_short | <b>Extraction, physical-chemical characterization and <i>in vitro</i> inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
| title_sort | b extraction physical chemical characterization and i in vitro i inhibitory potential of thrombin generation of crude sulfated polysaccharides from brazilian tropical seaweeds |
| topic | marine algae polyglycans coagulation tests thrombin. |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463 |
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