Fluorogen-activating proteins: beyond classical fluorescent proteins
Fluorescence imaging is a powerful technique for the real-time noninvasive monitoring of protein dynamics. Recently, fluorogen activating proteins (FAPs)/fluorogen probes for protein imaging were developed. Unlike the traditional fluorescent proteins (FPs), FAPs do not fluoresce unless bound to thei...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-05-01
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| Series: | Acta Pharmaceutica Sinica B |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221138351730597X |
| _version_ | 1819128541841719296 |
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| author | Shengnan Xu Hai-Yu Hu |
| author_facet | Shengnan Xu Hai-Yu Hu |
| author_sort | Shengnan Xu |
| collection | DOAJ |
| description | Fluorescence imaging is a powerful technique for the real-time noninvasive monitoring of protein dynamics. Recently, fluorogen activating proteins (FAPs)/fluorogen probes for protein imaging were developed. Unlike the traditional fluorescent proteins (FPs), FAPs do not fluoresce unless bound to their specific small-molecule fluorogens. When using FAPs/fluorogen probes, a washing step is not required for the removal of free probes from the cells, thus allowing rapid and specific detection of proteins in living cells with high signal-to-noise ratio. Furthermore, with different fluorogens, living cell multi-color proteins labeling system was developed. In this review, we describe about the discovery of FAPs, the design strategy of FAP fluorogens, the application of the FAP technology and the advances of FAP technology in protein labeling systems. KEY WORDS: Fluorogen activating proteins, Fluorogens, Genetically encoded sensors, Fluorescence imaging, Molecular imaging |
| first_indexed | 2024-12-22T08:29:28Z |
| format | Article |
| id | doaj.art-12127a93e0904557b3eb4e7820318d0c |
| institution | Directory Open Access Journal |
| issn | 2211-3835 |
| language | English |
| last_indexed | 2024-12-22T08:29:28Z |
| publishDate | 2018-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-12127a93e0904557b3eb4e7820318d0c2022-12-21T18:32:33ZengElsevierActa Pharmaceutica Sinica B2211-38352018-05-0183339348Fluorogen-activating proteins: beyond classical fluorescent proteinsShengnan Xu0Hai-Yu Hu1State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, ChinaState Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China; Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China; Corresponding author at: State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China.Fluorescence imaging is a powerful technique for the real-time noninvasive monitoring of protein dynamics. Recently, fluorogen activating proteins (FAPs)/fluorogen probes for protein imaging were developed. Unlike the traditional fluorescent proteins (FPs), FAPs do not fluoresce unless bound to their specific small-molecule fluorogens. When using FAPs/fluorogen probes, a washing step is not required for the removal of free probes from the cells, thus allowing rapid and specific detection of proteins in living cells with high signal-to-noise ratio. Furthermore, with different fluorogens, living cell multi-color proteins labeling system was developed. In this review, we describe about the discovery of FAPs, the design strategy of FAP fluorogens, the application of the FAP technology and the advances of FAP technology in protein labeling systems. KEY WORDS: Fluorogen activating proteins, Fluorogens, Genetically encoded sensors, Fluorescence imaging, Molecular imaginghttp://www.sciencedirect.com/science/article/pii/S221138351730597X |
| spellingShingle | Shengnan Xu Hai-Yu Hu Fluorogen-activating proteins: beyond classical fluorescent proteins Acta Pharmaceutica Sinica B |
| title | Fluorogen-activating proteins: beyond classical fluorescent proteins |
| title_full | Fluorogen-activating proteins: beyond classical fluorescent proteins |
| title_fullStr | Fluorogen-activating proteins: beyond classical fluorescent proteins |
| title_full_unstemmed | Fluorogen-activating proteins: beyond classical fluorescent proteins |
| title_short | Fluorogen-activating proteins: beyond classical fluorescent proteins |
| title_sort | fluorogen activating proteins beyond classical fluorescent proteins |
| url | http://www.sciencedirect.com/science/article/pii/S221138351730597X |
| work_keys_str_mv | AT shengnanxu fluorogenactivatingproteinsbeyondclassicalfluorescentproteins AT haiyuhu fluorogenactivatingproteinsbeyondclassicalfluorescentproteins |