Fragility in the 14q21q translocation region
Aphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicat...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2002-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300003 |
| _version_ | 1819074239951536128 |
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| author | Stacy R. Denison Asha S. Multani Sen Pathak Ira F. Greenbaum |
| author_facet | Stacy R. Denison Asha S. Multani Sen Pathak Ira F. Greenbaum |
| author_sort | Stacy R. Denison |
| collection | DOAJ |
| description | Aphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicated the absence of beta-satellite signal at the translocation region. Per-individual analysis of metaphases from APC-treated peripheral blood lymphocyte cultures identified significantly nonrandom chromosomal breakage at the translocation region in all three individuals examined. The APC-inducible fragility at the 14q21q translocation region suggests that this rearrangement was the result of chromosomal mutation at fragile site(s) in the progenitor chromosomes, or that this fragility was the result of the fusion of nonfragile progenitor chromosomes. |
| first_indexed | 2024-12-21T18:06:22Z |
| format | Article |
| id | doaj.art-1213db2bb107447e9d5af730235c129a |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T18:06:22Z |
| publishDate | 2002-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-1213db2bb107447e9d5af730235c129a2022-12-21T18:54:55ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852002-01-0125327127610.1590/S1415-47572002000300003Fragility in the 14q21q translocation regionStacy R. DenisonAsha S. MultaniSen PathakIra F. GreenbaumAphidicolin (APC)-induced chromosomal breakage was analyzed for women representing three generations of a single family and carrying a Robertsonian translocation rob(14q21q). Fluorescence in situ hybridization (FISH) analysis confirmed the dicentric constitution of the derived chromosome and indicated the absence of beta-satellite signal at the translocation region. Per-individual analysis of metaphases from APC-treated peripheral blood lymphocyte cultures identified significantly nonrandom chromosomal breakage at the translocation region in all three individuals examined. The APC-inducible fragility at the 14q21q translocation region suggests that this rearrangement was the result of chromosomal mutation at fragile site(s) in the progenitor chromosomes, or that this fragility was the result of the fusion of nonfragile progenitor chromosomes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300003rob(14q21q)Robertsonian translocationfragile sitesaphidicolinheritability |
| spellingShingle | Stacy R. Denison Asha S. Multani Sen Pathak Ira F. Greenbaum Fragility in the 14q21q translocation region Genetics and Molecular Biology rob(14q21q) Robertsonian translocation fragile sites aphidicolin heritability |
| title | Fragility in the 14q21q translocation region |
| title_full | Fragility in the 14q21q translocation region |
| title_fullStr | Fragility in the 14q21q translocation region |
| title_full_unstemmed | Fragility in the 14q21q translocation region |
| title_short | Fragility in the 14q21q translocation region |
| title_sort | fragility in the 14q21q translocation region |
| topic | rob(14q21q) Robertsonian translocation fragile sites aphidicolin heritability |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300003 |
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