Stability of polymeric cationic niosomes and their plasmid DNA-based complexes as gene delivery carriers
AbstractThis study aims to explore the stability of lipo-polymeric niosomes/niosome-based pCMS-EGFP complexes under different storage temperatures (25 °C, 4 °C, and −20 °C). To date, the question of nucleic acid-complex stability is one of the most vital issues in gene delivery applications. The nee...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Drug Delivery |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10717544.2023.2219420 |
| _version_ | 1797260977857626112 |
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| author | Mohamed Mashal Noha Attia Santiago Grijalvo Ramón Eritja Gustavo Puras José Luis Pedraz |
| author_facet | Mohamed Mashal Noha Attia Santiago Grijalvo Ramón Eritja Gustavo Puras José Luis Pedraz |
| author_sort | Mohamed Mashal |
| collection | DOAJ |
| description | AbstractThis study aims to explore the stability of lipo-polymeric niosomes/niosome-based pCMS-EGFP complexes under different storage temperatures (25 °C, 4 °C, and −20 °C). To date, the question of nucleic acid-complex stability is one of the most vital issues in gene delivery applications. The need for stable vaccines during the COVID-19 pandemic has merely highlighted it. In the case of niosomes as gene carriers, the scientific literature still lacks comprehensive stability studies. In this study, the physicochemical features of niosomes/nioplexes in terms of size, surface charge, and polydispersity index (PDI), along with transfection efficiency, and cytotoxicity in NT2 cells were evaluated for 8 weeks. Compared to day 0, the physicochemical features of the niosomes stored at 25 °C and −20 °C changed dramatically in terms of size, zeta potential, and PDI, while remaining in reasonable values when stored at 4 °C. However, niosomes and nioplexes stored at 4 °C and −20 °C showed nearly stable transfection efficiency values, yet an obvious decrease at 25 °C. This article provides a proof of concept into the stability of polymeric cationic niosomes and their nioplexes as promising gene delivery vehicles. Moreover, it highlights the practical possibility of storing nioplexes at 4 °C for up to 2 months, as an alternative to niosomes, for gene delivery purposes. |
| first_indexed | 2024-03-08T23:31:24Z |
| format | Article |
| id | doaj.art-12143e432e7544cba65da8fd7b01828f |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-04-24T23:33:54Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-12143e432e7544cba65da8fd7b01828f2024-03-15T14:22:17ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642023-12-0130110.1080/10717544.2023.2219420Stability of polymeric cationic niosomes and their plasmid DNA-based complexes as gene delivery carriersMohamed Mashal0Noha Attia1Santiago Grijalvo2Ramón Eritja3Gustavo Puras4José Luis Pedraz5NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, SpainNanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, SpainNetworking Research Centre of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, SpainNetworking Research Centre of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, SpainNanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, SpainNanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, SpainAbstractThis study aims to explore the stability of lipo-polymeric niosomes/niosome-based pCMS-EGFP complexes under different storage temperatures (25 °C, 4 °C, and −20 °C). To date, the question of nucleic acid-complex stability is one of the most vital issues in gene delivery applications. The need for stable vaccines during the COVID-19 pandemic has merely highlighted it. In the case of niosomes as gene carriers, the scientific literature still lacks comprehensive stability studies. In this study, the physicochemical features of niosomes/nioplexes in terms of size, surface charge, and polydispersity index (PDI), along with transfection efficiency, and cytotoxicity in NT2 cells were evaluated for 8 weeks. Compared to day 0, the physicochemical features of the niosomes stored at 25 °C and −20 °C changed dramatically in terms of size, zeta potential, and PDI, while remaining in reasonable values when stored at 4 °C. However, niosomes and nioplexes stored at 4 °C and −20 °C showed nearly stable transfection efficiency values, yet an obvious decrease at 25 °C. This article provides a proof of concept into the stability of polymeric cationic niosomes and their nioplexes as promising gene delivery vehicles. Moreover, it highlights the practical possibility of storing nioplexes at 4 °C for up to 2 months, as an alternative to niosomes, for gene delivery purposes.https://www.tandfonline.com/doi/10.1080/10717544.2023.2219420Stabilitynon-viral vectorsgene deliverycationic niosomenioplexes |
| spellingShingle | Mohamed Mashal Noha Attia Santiago Grijalvo Ramón Eritja Gustavo Puras José Luis Pedraz Stability of polymeric cationic niosomes and their plasmid DNA-based complexes as gene delivery carriers Drug Delivery Stability non-viral vectors gene delivery cationic niosome nioplexes |
| title | Stability of polymeric cationic niosomes and their plasmid DNA-based complexes as gene delivery carriers |
| title_full | Stability of polymeric cationic niosomes and their plasmid DNA-based complexes as gene delivery carriers |
| title_fullStr | Stability of polymeric cationic niosomes and their plasmid DNA-based complexes as gene delivery carriers |
| title_full_unstemmed | Stability of polymeric cationic niosomes and their plasmid DNA-based complexes as gene delivery carriers |
| title_short | Stability of polymeric cationic niosomes and their plasmid DNA-based complexes as gene delivery carriers |
| title_sort | stability of polymeric cationic niosomes and their plasmid dna based complexes as gene delivery carriers |
| topic | Stability non-viral vectors gene delivery cationic niosome nioplexes |
| url | https://www.tandfonline.com/doi/10.1080/10717544.2023.2219420 |
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