Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications
The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, alt...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-06-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2306-5354/5/2/43 |
| _version_ | 1797708784634691584 |
|---|---|
| author | Katharina Schimek Hao-Hsiang Hsu Moritz Boehme Jacob Jan Kornet Uwe Marx Roland Lauster Ralf Pörtner Gerd Lindner |
| author_facet | Katharina Schimek Hao-Hsiang Hsu Moritz Boehme Jacob Jan Kornet Uwe Marx Roland Lauster Ralf Pörtner Gerd Lindner |
| author_sort | Katharina Schimek |
| collection | DOAJ |
| description | The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attractive alternative to replace animal testing. The two-organ chip is designed to hold 96-well cell culture inserts (CCIs). Small-sized skin equivalents are needed for this. In this study, full-thickness skin equivalents (ftSEs) were generated successfully inside 96-well CCIs. These skin equivalents developed with in vivo-like histological architecture, with normal differentiation marker expressions and proliferation rates. The 96-well CCI-based ftSEs were successfully integrated into the two-organ chip. The permeation of fluorescein sodium salt through the ftSEs was monitored during the culture. The results show a decreasing value for the permeation over time, which seems a promising method to track the development of the ftSEs. Additionally, the permeation was implemented in a computational fluid dynamics simulation, as a tool to predict results in long-term experiments. The advantage of these ftSEs is the reduced need for cells and substances, which makes them more suitable for high throughput assays. |
| first_indexed | 2024-03-12T06:27:42Z |
| format | Article |
| id | doaj.art-121443439f8a498889d8f40c95ccf1d8 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T06:27:42Z |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-121443439f8a498889d8f40c95ccf1d82023-09-03T01:48:48ZengMDPI AGBioengineering2306-53542018-06-01524310.3390/bioengineering5020043bioengineering5020043Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip ApplicationsKatharina Schimek0Hao-Hsiang Hsu1Moritz Boehme2Jacob Jan Kornet3Uwe Marx4Roland Lauster5Ralf Pörtner6Gerd Lindner7TissUse GmbH, D-13347 Berlin, GermanyInstitute of Bioprocess and Biosystem Engineering, Hamburg University of Technology, D-21073 Hamburg, GermanyInstitute of Bioprocess and Biosystem Engineering, Hamburg University of Technology, D-21073 Hamburg, GermanyInstitute of Bioprocess and Biosystem Engineering, Hamburg University of Technology, D-21073 Hamburg, GermanyTissUse GmbH, D-13347 Berlin, GermanyInstitute of Biotechnology, Department Medical Biotechnology, Technische Universität Berlin, D-13355 Berlin, GermanyInstitute of Bioprocess and Biosystem Engineering, Hamburg University of Technology, D-21073 Hamburg, GermanyInstitute of Biotechnology, Department Medical Biotechnology, Technische Universität Berlin, D-13355 Berlin, GermanyThe human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attractive alternative to replace animal testing. The two-organ chip is designed to hold 96-well cell culture inserts (CCIs). Small-sized skin equivalents are needed for this. In this study, full-thickness skin equivalents (ftSEs) were generated successfully inside 96-well CCIs. These skin equivalents developed with in vivo-like histological architecture, with normal differentiation marker expressions and proliferation rates. The 96-well CCI-based ftSEs were successfully integrated into the two-organ chip. The permeation of fluorescein sodium salt through the ftSEs was monitored during the culture. The results show a decreasing value for the permeation over time, which seems a promising method to track the development of the ftSEs. Additionally, the permeation was implemented in a computational fluid dynamics simulation, as a tool to predict results in long-term experiments. The advantage of these ftSEs is the reduced need for cells and substances, which makes them more suitable for high throughput assays.http://www.mdpi.com/2306-5354/5/2/43full thickness skin equivalentsmulti-organ chipsubstance permeation96-well cell culture insert |
| spellingShingle | Katharina Schimek Hao-Hsiang Hsu Moritz Boehme Jacob Jan Kornet Uwe Marx Roland Lauster Ralf Pörtner Gerd Lindner Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications Bioengineering full thickness skin equivalents multi-organ chip substance permeation 96-well cell culture insert |
| title | Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications |
| title_full | Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications |
| title_fullStr | Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications |
| title_full_unstemmed | Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications |
| title_short | Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications |
| title_sort | bioengineering of a full thickness skin equivalent in a 96 well insert format for substance permeation studies and organ on a chip applications |
| topic | full thickness skin equivalents multi-organ chip substance permeation 96-well cell culture insert |
| url | http://www.mdpi.com/2306-5354/5/2/43 |
| work_keys_str_mv | AT katharinaschimek bioengineeringofafullthicknessskinequivalentina96wellinsertformatforsubstancepermeationstudiesandorganonachipapplications AT haohsianghsu bioengineeringofafullthicknessskinequivalentina96wellinsertformatforsubstancepermeationstudiesandorganonachipapplications AT moritzboehme bioengineeringofafullthicknessskinequivalentina96wellinsertformatforsubstancepermeationstudiesandorganonachipapplications AT jacobjankornet bioengineeringofafullthicknessskinequivalentina96wellinsertformatforsubstancepermeationstudiesandorganonachipapplications AT uwemarx bioengineeringofafullthicknessskinequivalentina96wellinsertformatforsubstancepermeationstudiesandorganonachipapplications AT rolandlauster bioengineeringofafullthicknessskinequivalentina96wellinsertformatforsubstancepermeationstudiesandorganonachipapplications AT ralfportner bioengineeringofafullthicknessskinequivalentina96wellinsertformatforsubstancepermeationstudiesandorganonachipapplications AT gerdlindner bioengineeringofafullthicknessskinequivalentina96wellinsertformatforsubstancepermeationstudiesandorganonachipapplications |