Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity
Parkinson’s disease (PD) is the second most common progressive neurodegenerative disease, after Alzheimer’s disease. In our previous study, we found that amber—a fossilized plant resin—can protect cells from apoptosis by decreasing the generation of reactive oxygen species (ROS). In this study, we f...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/6/1817 |
| _version_ | 1827627290258833408 |
|---|---|
| author | Yuening Luo Siqi Zhou Reiko Takeda Kazuma Okazaki Marie Sekita Kazuichi Sakamoto |
| author_facet | Yuening Luo Siqi Zhou Reiko Takeda Kazuma Okazaki Marie Sekita Kazuichi Sakamoto |
| author_sort | Yuening Luo |
| collection | DOAJ |
| description | Parkinson’s disease (PD) is the second most common progressive neurodegenerative disease, after Alzheimer’s disease. In our previous study, we found that amber—a fossilized plant resin—can protect cells from apoptosis by decreasing the generation of reactive oxygen species (ROS). In this study, we focused on the effect of amber on 6-hydroxydopamine-induced cell apoptosis in the human neuroblastoma cell line SHSY5Y (one model for PD). Initially, we determined the protective effect of amber on the PD model. We found that amber extract has a protective effect against 6-hydroxydopamine-induced cell apoptosis. The decrease in ROS, cleaved caspase-3, pERK, and extracellular signal-regulated kinase (ERK) protein levels confirmed that amber extract decreases apoptosis via the ROS-mediated ERK signaling pathway. Furthermore, we determined the effects of amber extract on autophagy. The results showed that amber extract increased the levels of LC3II and Beclin-1, suggesting that amber extract can protect neuronal cells against 6-hydroxydopamine-induced cell apoptosis by promoting autophagy. |
| first_indexed | 2024-03-09T13:11:15Z |
| format | Article |
| id | doaj.art-1216dec23a4b4a40aeb0a86cc0a86c9e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T13:11:15Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1216dec23a4b4a40aeb0a86cc0a86c9e2023-11-30T21:41:38ZengMDPI AGMolecules1420-30492022-03-01276181710.3390/molecules27061817Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced NeurotoxicityYuening Luo0Siqi Zhou1Reiko Takeda2Kazuma Okazaki3Marie Sekita4Kazuichi Sakamoto5Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, JapanGraduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, JapanGraduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, JapanResearch and Development, Kohaku Bio Technology Co., Ltd., Tsukuba 305-8572, Ibaraki, JapanResearch and Development, Kohaku Bio Technology Co., Ltd., Tsukuba 305-8572, Ibaraki, JapanGraduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, JapanParkinson’s disease (PD) is the second most common progressive neurodegenerative disease, after Alzheimer’s disease. In our previous study, we found that amber—a fossilized plant resin—can protect cells from apoptosis by decreasing the generation of reactive oxygen species (ROS). In this study, we focused on the effect of amber on 6-hydroxydopamine-induced cell apoptosis in the human neuroblastoma cell line SHSY5Y (one model for PD). Initially, we determined the protective effect of amber on the PD model. We found that amber extract has a protective effect against 6-hydroxydopamine-induced cell apoptosis. The decrease in ROS, cleaved caspase-3, pERK, and extracellular signal-regulated kinase (ERK) protein levels confirmed that amber extract decreases apoptosis via the ROS-mediated ERK signaling pathway. Furthermore, we determined the effects of amber extract on autophagy. The results showed that amber extract increased the levels of LC3II and Beclin-1, suggesting that amber extract can protect neuronal cells against 6-hydroxydopamine-induced cell apoptosis by promoting autophagy.https://www.mdpi.com/1420-3049/27/6/18176-hydroxydopamineamberextracellular signal-regulated kinase pathwayautophagy |
| spellingShingle | Yuening Luo Siqi Zhou Reiko Takeda Kazuma Okazaki Marie Sekita Kazuichi Sakamoto Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity Molecules 6-hydroxydopamine amber extracellular signal-regulated kinase pathway autophagy |
| title | Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity |
| title_full | Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity |
| title_fullStr | Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity |
| title_full_unstemmed | Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity |
| title_short | Protective Effect of Amber Extract on Human Dopaminergic Cells against 6-Hydroxydopamine-Induced Neurotoxicity |
| title_sort | protective effect of amber extract on human dopaminergic cells against 6 hydroxydopamine induced neurotoxicity |
| topic | 6-hydroxydopamine amber extracellular signal-regulated kinase pathway autophagy |
| url | https://www.mdpi.com/1420-3049/27/6/1817 |
| work_keys_str_mv | AT yueningluo protectiveeffectofamberextractonhumandopaminergiccellsagainst6hydroxydopamineinducedneurotoxicity AT siqizhou protectiveeffectofamberextractonhumandopaminergiccellsagainst6hydroxydopamineinducedneurotoxicity AT reikotakeda protectiveeffectofamberextractonhumandopaminergiccellsagainst6hydroxydopamineinducedneurotoxicity AT kazumaokazaki protectiveeffectofamberextractonhumandopaminergiccellsagainst6hydroxydopamineinducedneurotoxicity AT mariesekita protectiveeffectofamberextractonhumandopaminergiccellsagainst6hydroxydopamineinducedneurotoxicity AT kazuichisakamoto protectiveeffectofamberextractonhumandopaminergiccellsagainst6hydroxydopamineinducedneurotoxicity |