COVID-19 Vaccine mRNABNT162b2 Elicits Human Antibody Response in Milk of Breastfeeding Women
Objective: The objective of this research is to demonstrate the release of SARS-CoV-2 Spike (S) antibodies in human milk samples obtained by patients who have been vaccinated with mRNABNT162b2 vaccine. Methods: Milk and serum samples were collected in 10 volunteers 20 days after the first dose and 7...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/9/7/785 |
| _version_ | 1797525893464195072 |
|---|---|
| author | Maurizio Guida Daniela Terracciano Michele Cennamo Federica Aiello Evelina La Civita Gennaro Esposito Valentina Gargiulo Giuseppe M. Maruotti Giuseppe Portella Laura Sarno |
| author_facet | Maurizio Guida Daniela Terracciano Michele Cennamo Federica Aiello Evelina La Civita Gennaro Esposito Valentina Gargiulo Giuseppe M. Maruotti Giuseppe Portella Laura Sarno |
| author_sort | Maurizio Guida |
| collection | DOAJ |
| description | Objective: The objective of this research is to demonstrate the release of SARS-CoV-2 Spike (S) antibodies in human milk samples obtained by patients who have been vaccinated with mRNABNT162b2 vaccine. Methods: Milk and serum samples were collected in 10 volunteers 20 days after the first dose and 7 seven days after the second dose of the mRNABNT162b2 vaccine. Anti-SARS-CoV-2 S antibodies were measured by the Elecsys<sup>®</sup> Anti-SARS-CoV-2 S ECLIA assay (Roche Diagnostics AG, Rotkreuz, Switzerland), a quantitative electrochemiluminescence immunometric method. Results: At first sample, anti-SARS-CoV-2 S antibodies were detected in all serum samples (103.9 ± 54.9 U/mL) and only in two (40%) milk samples with a low concentration (1.2 ± 0.3 U/mL). At the second sample, collected 7 days after the second dose, anti-SARS-CoV-2 S antibodies were detected in all serum samples (3875.7 ± 3504.6 UI/mL) and in all milk samples (41.5 ± 47.5 UI/mL). No correlation was found between the level of serum and milk antibodies; the milk antibodies/serum antibodies ratio was on average 2% (range: 0.2–8.4%). Conclusion: We demonstrated a release of anti-SARS-CoV-2 S antibodies in the breast milk of women vaccinated with mRNABNT162b2. Vaccinating breastfeeding women could be a strategy to protect their infants from COVID-19 infection. |
| first_indexed | 2024-03-10T09:20:26Z |
| format | Article |
| id | doaj.art-121823a02184454eb5e98bc02313f2a8 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T09:20:26Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-121823a02184454eb5e98bc02313f2a82023-11-22T05:13:01ZengMDPI AGVaccines2076-393X2021-07-019778510.3390/vaccines9070785COVID-19 Vaccine mRNABNT162b2 Elicits Human Antibody Response in Milk of Breastfeeding WomenMaurizio Guida0Daniela Terracciano1Michele Cennamo2Federica Aiello3Evelina La Civita4Gennaro Esposito5Valentina Gargiulo6Giuseppe M. Maruotti7Giuseppe Portella8Laura Sarno9Department of Neurosciences, Reproductive Science and Dentistry, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Neurosciences, Reproductive Science and Dentistry, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Public Health, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Mother and Child, University Hospital Federico II, 80131 Naples, ItalyDepartment of Neurosciences, Reproductive Science and Dentistry, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Translational Medical Sciences, University of Naples “Federico II”, 80131 Naples, ItalyDepartment of Neurosciences, Reproductive Science and Dentistry, University of Naples “Federico II”, 80131 Naples, ItalyObjective: The objective of this research is to demonstrate the release of SARS-CoV-2 Spike (S) antibodies in human milk samples obtained by patients who have been vaccinated with mRNABNT162b2 vaccine. Methods: Milk and serum samples were collected in 10 volunteers 20 days after the first dose and 7 seven days after the second dose of the mRNABNT162b2 vaccine. Anti-SARS-CoV-2 S antibodies were measured by the Elecsys<sup>®</sup> Anti-SARS-CoV-2 S ECLIA assay (Roche Diagnostics AG, Rotkreuz, Switzerland), a quantitative electrochemiluminescence immunometric method. Results: At first sample, anti-SARS-CoV-2 S antibodies were detected in all serum samples (103.9 ± 54.9 U/mL) and only in two (40%) milk samples with a low concentration (1.2 ± 0.3 U/mL). At the second sample, collected 7 days after the second dose, anti-SARS-CoV-2 S antibodies were detected in all serum samples (3875.7 ± 3504.6 UI/mL) and in all milk samples (41.5 ± 47.5 UI/mL). No correlation was found between the level of serum and milk antibodies; the milk antibodies/serum antibodies ratio was on average 2% (range: 0.2–8.4%). Conclusion: We demonstrated a release of anti-SARS-CoV-2 S antibodies in the breast milk of women vaccinated with mRNABNT162b2. Vaccinating breastfeeding women could be a strategy to protect their infants from COVID-19 infection.https://www.mdpi.com/2076-393X/9/7/785vaccinemilkimmunoglobulinsSARS-Cov-2 |
| spellingShingle | Maurizio Guida Daniela Terracciano Michele Cennamo Federica Aiello Evelina La Civita Gennaro Esposito Valentina Gargiulo Giuseppe M. Maruotti Giuseppe Portella Laura Sarno COVID-19 Vaccine mRNABNT162b2 Elicits Human Antibody Response in Milk of Breastfeeding Women Vaccines vaccine milk immunoglobulins SARS-Cov-2 |
| title | COVID-19 Vaccine mRNABNT162b2 Elicits Human Antibody Response in Milk of Breastfeeding Women |
| title_full | COVID-19 Vaccine mRNABNT162b2 Elicits Human Antibody Response in Milk of Breastfeeding Women |
| title_fullStr | COVID-19 Vaccine mRNABNT162b2 Elicits Human Antibody Response in Milk of Breastfeeding Women |
| title_full_unstemmed | COVID-19 Vaccine mRNABNT162b2 Elicits Human Antibody Response in Milk of Breastfeeding Women |
| title_short | COVID-19 Vaccine mRNABNT162b2 Elicits Human Antibody Response in Milk of Breastfeeding Women |
| title_sort | covid 19 vaccine mrnabnt162b2 elicits human antibody response in milk of breastfeeding women |
| topic | vaccine milk immunoglobulins SARS-Cov-2 |
| url | https://www.mdpi.com/2076-393X/9/7/785 |
| work_keys_str_mv | AT maurizioguida covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen AT danielaterracciano covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen AT michelecennamo covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen AT federicaaiello covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen AT evelinalacivita covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen AT gennaroesposito covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen AT valentinagargiulo covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen AT giuseppemmaruotti covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen AT giuseppeportella covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen AT laurasarno covid19vaccinemrnabnt162b2elicitshumanantibodyresponseinmilkofbreastfeedingwomen |