Multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cells
Abstract Cigarette smoke (CS) exposure is known to cause injury to respiratory tract epithelial cells and is a contributing factor in the development of chronic obstructive pulmonary disease and lung cancer. Electronic cigarettes (e-cigarettes) are gaining popularity as a potential substitute for co...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-55140-3 |
| _version_ | 1797275121305518080 |
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| author | Meng Wang Qing Cheng Zehong Wu Longjiang Fan Linghui Zeng Hongyu Chen |
| author_facet | Meng Wang Qing Cheng Zehong Wu Longjiang Fan Linghui Zeng Hongyu Chen |
| author_sort | Meng Wang |
| collection | DOAJ |
| description | Abstract Cigarette smoke (CS) exposure is known to cause injury to respiratory tract epithelial cells and is a contributing factor in the development of chronic obstructive pulmonary disease and lung cancer. Electronic cigarettes (e-cigarettes) are gaining popularity as a potential substitute for conventional cigarettes due to their potential for aiding smoking cessation. However, the safety of e-cigarettes remains uncertain, and scientific evidence on this topic is still limited. In this study, we aimed to investigate the effects of CS and e-cigarette smoke (ECS) of different flavors on human lung bronchial epithelial cells. Real-time smoke exposure was carried out using an air–liquid interface system, and cell viability was assessed. RNA-Seq transcriptome analysis was performed to compare the differences between CS and ECS. The transcriptome analysis revealed a significantly higher number of differentially expressed genes in CS than in ECS. Moreover, the impact of mint-flavored e-cigarettes on cells was found to be greater than that of tobacco-flavored e-cigarettes, as evidenced by the greater number of differentially expressed genes. These findings provide a reference for future safety research on traditional cigarettes and e-cigarettes, particularly those of different flavors. The use of omics-scale methodologies has improved our ability to understand the biological effects of CS and ECS on human respiratory tract epithelial cells, which can aid in the development of novel approaches for smoking cessation and lung disease prevention. |
| first_indexed | 2024-03-07T15:08:48Z |
| format | Article |
| id | doaj.art-1218310da573492b9d4b5b673e5d35a2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:08:48Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1218310da573492b9d4b5b673e5d35a22024-03-05T18:44:39ZengNature PortfolioScientific Reports2045-23222024-02-0114111210.1038/s41598-024-55140-3Multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cellsMeng Wang0Qing Cheng1Zehong Wu2Longjiang Fan3Linghui Zeng4Hongyu Chen5Hangzhou Center for Disease Control and PreventionInstitute of Bioinformatics, Zhejiang UniversityRELX Science Center, Shenzhen RELX Tech. Co. Ltd.Institute of Bioinformatics, Zhejiang UniversitySchool of Medicine, Hangzhou City UniversityInstitute of Bioinformatics, Zhejiang UniversityAbstract Cigarette smoke (CS) exposure is known to cause injury to respiratory tract epithelial cells and is a contributing factor in the development of chronic obstructive pulmonary disease and lung cancer. Electronic cigarettes (e-cigarettes) are gaining popularity as a potential substitute for conventional cigarettes due to their potential for aiding smoking cessation. However, the safety of e-cigarettes remains uncertain, and scientific evidence on this topic is still limited. In this study, we aimed to investigate the effects of CS and e-cigarette smoke (ECS) of different flavors on human lung bronchial epithelial cells. Real-time smoke exposure was carried out using an air–liquid interface system, and cell viability was assessed. RNA-Seq transcriptome analysis was performed to compare the differences between CS and ECS. The transcriptome analysis revealed a significantly higher number of differentially expressed genes in CS than in ECS. Moreover, the impact of mint-flavored e-cigarettes on cells was found to be greater than that of tobacco-flavored e-cigarettes, as evidenced by the greater number of differentially expressed genes. These findings provide a reference for future safety research on traditional cigarettes and e-cigarettes, particularly those of different flavors. The use of omics-scale methodologies has improved our ability to understand the biological effects of CS and ECS on human respiratory tract epithelial cells, which can aid in the development of novel approaches for smoking cessation and lung disease prevention.https://doi.org/10.1038/s41598-024-55140-3Cigarette smokeElectronic cigarettesBronchial epithelial cellsTranscriptomic analysisMint flavor |
| spellingShingle | Meng Wang Qing Cheng Zehong Wu Longjiang Fan Linghui Zeng Hongyu Chen Multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cells Scientific Reports Cigarette smoke Electronic cigarettes Bronchial epithelial cells Transcriptomic analysis Mint flavor |
| title | Multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cells |
| title_full | Multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cells |
| title_fullStr | Multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cells |
| title_full_unstemmed | Multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cells |
| title_short | Multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cells |
| title_sort | multidimensional assessment of the biological effects of electronic cigarettes on lung bronchial epithelial cells |
| topic | Cigarette smoke Electronic cigarettes Bronchial epithelial cells Transcriptomic analysis Mint flavor |
| url | https://doi.org/10.1038/s41598-024-55140-3 |
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