Mechanistic exploration of Yiqi Liangxue Shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacology
AbstractContext Yiqi Liangxue Shengji prescription (YQLXSJ) is a traditional Chinese medicine (TCM) formula that has long been used for treatment after percutaneous coronary intervention (PCI).Objective To investigate the putative pharmacological mechanism of YQLXSJ on restenosis through an integrat...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2023.2244533 |
| _version_ | 1797234812603334656 |
|---|---|
| author | Tianshi Mao Long Xie Yanqiong Guo Xiang Ji Jie Wan Xiaoyun Cui Qian Fan Wei Liu Shuai Wang Wenbo Han Qian Lin Wenhao Jia |
| author_facet | Tianshi Mao Long Xie Yanqiong Guo Xiang Ji Jie Wan Xiaoyun Cui Qian Fan Wei Liu Shuai Wang Wenbo Han Qian Lin Wenhao Jia |
| author_sort | Tianshi Mao |
| collection | DOAJ |
| description | AbstractContext Yiqi Liangxue Shengji prescription (YQLXSJ) is a traditional Chinese medicine (TCM) formula that has long been used for treatment after percutaneous coronary intervention (PCI).Objective To investigate the putative pharmacological mechanism of YQLXSJ on restenosis through an integrated approach utilizing metabolomics and network pharmacology.Materials and methods Forty male Sprague–Dawley rats were divided into sham, model, YQLXSJ, and positive groups. YQLXSJ group received the treatment of YQLXSJ (6 g/kg/d, i.g.) and the positive group was treated with atorvastatin (2 mg/kg/d, i.g.). After 4 weeks, the improvement in intimal hyperplasia was evaluated by ultrasound, H&E staining, and immunofluorescence. UPLC–MS/MS technology was utilized to screen the differential metabolites. Network pharmacology was conducted using TCMSP, GeneCards, and Metascape, etc., in combination with metabolomics. Eventually, the core targets were acquired and validated.Results Compared to models, YQLXSJ exhibited decreased intima-media thickness on ultrasound (0.23 ± 0.02 mm vs. 0.20 ± 0.01 mm, p < 0.01) and reduced intima thickness by H&E (30.12 ± 6.05 μm vs. 14.32 ± 1.37 μm, p < 0.01). We identified 18 differential metabolites and 5 core targets such as inducible nitric oxide synthase (NOS2), endothelial nitric oxide synthase (NOS3), vascular endothelial growth factor-A (VEGFA), ornithine decarboxylase-1 (ODC1) and group IIA secretory phospholipase A2 (PLA2G2A). These targets were further confirmed by molecular docking and ELISA.Discussion and conclusions This study confirms the effects of YQLXSJ on restenosis and reveals some biomarkers. TCM has great potential in the prevention and treatment of restenosis by improving metabolic disorders. |
| first_indexed | 2024-03-09T00:08:48Z |
| format | Article |
| id | doaj.art-12184705d9a642129a5e7f255f4801b4 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-24T16:38:01Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-12184705d9a642129a5e7f255f4801b42024-03-29T11:10:26ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162023-12-016111260127310.1080/13880209.2023.2244533Mechanistic exploration of Yiqi Liangxue Shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacologyTianshi Mao0Long Xie1Yanqiong Guo2Xiang Ji3Jie Wan4Xiaoyun Cui5Qian Fan6Wei Liu7Shuai Wang8Wenbo Han9Qian Lin10Wenhao Jia11Department of Cardiology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, P.R. ChinaDepartment of Cardiology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, P.R. ChinaDepartment of Cardiology, Beijing Fengtai District Hospital of Chinese Medicine, Beijing, P.R. ChinaDepartment of Cardiology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, P.R. ChinaDepartment of Cardiology, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, P.R. ChinaDepartment of Cardiology, Dongfang Hospital Beijing University of Chinese Medicine, Beijing, P.R. ChinaDepartment of Cardiology, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, P.R. ChinaDepartment of Cardiology, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, P.R. ChinaDepartment of Cardiology, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, P.R. ChinaDepartment of Cardiology, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, P.R. ChinaDepartment of Cardiology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, P.R. ChinaDepartment of Cardiology, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, P.R. ChinaAbstractContext Yiqi Liangxue Shengji prescription (YQLXSJ) is a traditional Chinese medicine (TCM) formula that has long been used for treatment after percutaneous coronary intervention (PCI).Objective To investigate the putative pharmacological mechanism of YQLXSJ on restenosis through an integrated approach utilizing metabolomics and network pharmacology.Materials and methods Forty male Sprague–Dawley rats were divided into sham, model, YQLXSJ, and positive groups. YQLXSJ group received the treatment of YQLXSJ (6 g/kg/d, i.g.) and the positive group was treated with atorvastatin (2 mg/kg/d, i.g.). After 4 weeks, the improvement in intimal hyperplasia was evaluated by ultrasound, H&E staining, and immunofluorescence. UPLC–MS/MS technology was utilized to screen the differential metabolites. Network pharmacology was conducted using TCMSP, GeneCards, and Metascape, etc., in combination with metabolomics. Eventually, the core targets were acquired and validated.Results Compared to models, YQLXSJ exhibited decreased intima-media thickness on ultrasound (0.23 ± 0.02 mm vs. 0.20 ± 0.01 mm, p < 0.01) and reduced intima thickness by H&E (30.12 ± 6.05 μm vs. 14.32 ± 1.37 μm, p < 0.01). We identified 18 differential metabolites and 5 core targets such as inducible nitric oxide synthase (NOS2), endothelial nitric oxide synthase (NOS3), vascular endothelial growth factor-A (VEGFA), ornithine decarboxylase-1 (ODC1) and group IIA secretory phospholipase A2 (PLA2G2A). These targets were further confirmed by molecular docking and ELISA.Discussion and conclusions This study confirms the effects of YQLXSJ on restenosis and reveals some biomarkers. TCM has great potential in the prevention and treatment of restenosis by improving metabolic disorders.https://www.tandfonline.com/doi/10.1080/13880209.2023.2244533Intimal hyperplasiatraditional Chinese medicineinteraction networkmetabolitesamino acid metabolism |
| spellingShingle | Tianshi Mao Long Xie Yanqiong Guo Xiang Ji Jie Wan Xiaoyun Cui Qian Fan Wei Liu Shuai Wang Wenbo Han Qian Lin Wenhao Jia Mechanistic exploration of Yiqi Liangxue Shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacology Pharmaceutical Biology Intimal hyperplasia traditional Chinese medicine interaction network metabolites amino acid metabolism |
| title | Mechanistic exploration of Yiqi Liangxue Shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacology |
| title_full | Mechanistic exploration of Yiqi Liangxue Shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacology |
| title_fullStr | Mechanistic exploration of Yiqi Liangxue Shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacology |
| title_full_unstemmed | Mechanistic exploration of Yiqi Liangxue Shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacology |
| title_short | Mechanistic exploration of Yiqi Liangxue Shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacology |
| title_sort | mechanistic exploration of yiqi liangxue shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacology |
| topic | Intimal hyperplasia traditional Chinese medicine interaction network metabolites amino acid metabolism |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2023.2244533 |
| work_keys_str_mv | AT tianshimao mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT longxie mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT yanqiongguo mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT xiangji mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT jiewan mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT xiaoyuncui mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT qianfan mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT weiliu mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT shuaiwang mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT wenbohan mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT qianlin mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology AT wenhaojia mechanisticexplorationofyiqiliangxueshengjiprescriptiononrestenosisafterballooninjurybyintegratingmetabolomicswithnetworkpharmacology |