Colorimetric determination of free fatty acids in biological fluids
Following the introduction by Duncombe of a colorimetric micromethod for the determination of free fatty acids (FFA) dissolved in chloroform, we studied the optimum conditions for chloroform extraction of FFA from blood or other biological fluids. A degree and rate of extraction similar to those wit...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1965-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520396334 |
| _version_ | 1818925301258780672 |
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| author | Koichi Itaya Michio Ui |
| author_facet | Koichi Itaya Michio Ui |
| author_sort | Koichi Itaya |
| collection | DOAJ |
| description | Following the introduction by Duncombe of a colorimetric micromethod for the determination of free fatty acids (FFA) dissolved in chloroform, we studied the optimum conditions for chloroform extraction of FFA from blood or other biological fluids. A degree and rate of extraction similar to those with Dole's extraction mixture were obtained.Phospholipids are not extracted by the procedure described. As little as 0.2 ml of whole blood, instead of serum or plasma, can serve as a FFA sample in the subsequent colorimetric determination, which has been modified to render it simpler and more sensitive. |
| first_indexed | 2024-12-20T02:39:03Z |
| format | Article |
| id | doaj.art-1218c062a9f14a0aad688afe69d27c17 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T02:39:03Z |
| publishDate | 1965-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-1218c062a9f14a0aad688afe69d27c172022-12-21T19:56:21ZengElsevierJournal of Lipid Research0022-22751965-01-01611620Colorimetric determination of free fatty acids in biological fluidsKoichi Itaya0Michio Ui1Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University School of Medicine, Sapporo, JapanDepartment of Biological Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University School of Medicine, Sapporo, JapanFollowing the introduction by Duncombe of a colorimetric micromethod for the determination of free fatty acids (FFA) dissolved in chloroform, we studied the optimum conditions for chloroform extraction of FFA from blood or other biological fluids. A degree and rate of extraction similar to those with Dole's extraction mixture were obtained.Phospholipids are not extracted by the procedure described. As little as 0.2 ml of whole blood, instead of serum or plasma, can serve as a FFA sample in the subsequent colorimetric determination, which has been modified to render it simpler and more sensitive.http://www.sciencedirect.com/science/article/pii/S0022227520396334free fatty acidsextractioncolorimetric determinationserumbloodcalcium |
| spellingShingle | Koichi Itaya Michio Ui Colorimetric determination of free fatty acids in biological fluids Journal of Lipid Research free fatty acids extraction colorimetric determination serum blood calcium |
| title | Colorimetric determination of free fatty acids in biological fluids |
| title_full | Colorimetric determination of free fatty acids in biological fluids |
| title_fullStr | Colorimetric determination of free fatty acids in biological fluids |
| title_full_unstemmed | Colorimetric determination of free fatty acids in biological fluids |
| title_short | Colorimetric determination of free fatty acids in biological fluids |
| title_sort | colorimetric determination of free fatty acids in biological fluids |
| topic | free fatty acids extraction colorimetric determination serum blood calcium |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520396334 |
| work_keys_str_mv | AT koichiitaya colorimetricdeterminationoffreefattyacidsinbiologicalfluids AT michioui colorimetricdeterminationoffreefattyacidsinbiologicalfluids |