Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model
<i>Cordyceps militaris</i> (<i>C. militaris</i>) has various biomedical applications in traditional oriental medicine for different diseases including inflammatory and immune-dysregulated diseases. It is a reservoir of nutritional components such as cordycepin, polysaccharide...
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MDPI AG
2021-10-01
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| Series: | Nutrients |
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| author | Abdul-Rehman Phull Kyu-Ree Dhong Hye-Jin Park |
| author_facet | Abdul-Rehman Phull Kyu-Ree Dhong Hye-Jin Park |
| author_sort | Abdul-Rehman Phull |
| collection | DOAJ |
| description | <i>Cordyceps militaris</i> (<i>C. militaris</i>) has various biomedical applications in traditional oriental medicine for different diseases including inflammatory and immune-dysregulated diseases. It is a reservoir of nutritional components such as cordycepin, polysaccharides, and antioxidants. To improve its bioactivity, we fermented <i>C. militaris</i> with a <i>Pediococcus pentosaceus</i> strain isolated from a salted small octopus (SC11). The current study aimed to evaluate whether <i>P. pentosaceus</i> (SC11) fermentation could enhance the anti-allergic potential of <i>C. militaris</i> cultured on germinated <i>Rhynchosia nulubilis</i> (GRC) against a type I hypersensitive reaction in in vitro and in vivo studies. Total antioxidant capacity and cordycepin content were significantly increased in GRC after SC11 fermentation. GRC-SC11 showed significantly enhanced anti-allergic responses by inhibiting immunoglobulin E (IgE)/antigen-induced degranulation in RBL-2H3 cells, compared to GRC. The results demonstrated the significant inhibition of phosphorylated spleen tyrosine kinase (Syk)/ p38/GRB2-associated binding protein 2 (Gab2)/c-jun in IgE/Ag-triggered RBL-2H3 cells. Furthermore, suppressed mRNA levels of interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) in IgE/Ag-activated RBL-2H3 cells were observed. GRC-SC11 significantly ameliorated IgE-induced allergic reactions by suppressing the ear swelling, vascular permeability, and inflammatory cell infiltration in passive cutaneous anaphylaxis (PCA) BALB/c mice. In conclusion, GRC fermented with <i>P.pentosaceus</i> exerted enhanced anti-allergic effects, and increased the cordycepin content and antioxidants potential compared to GRC. It can be used as bio-functional food in the prevention and management of type I allergic diseases. |
| first_indexed | 2024-03-10T05:11:34Z |
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| last_indexed | 2024-03-10T05:11:34Z |
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| series | Nutrients |
| spelling | doaj.art-121c3f1d572e4e56b3122871d9ff14e62023-11-23T00:46:51ZengMDPI AGNutrients2072-66432021-10-011311384910.3390/nu13113849Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine ModelAbdul-Rehman Phull0Kyu-Ree Dhong1Hye-Jin Park2Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi-do, KoreaDepartment of Life Science, College of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi-do, KoreaDepartment of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi-do, Korea<i>Cordyceps militaris</i> (<i>C. militaris</i>) has various biomedical applications in traditional oriental medicine for different diseases including inflammatory and immune-dysregulated diseases. It is a reservoir of nutritional components such as cordycepin, polysaccharides, and antioxidants. To improve its bioactivity, we fermented <i>C. militaris</i> with a <i>Pediococcus pentosaceus</i> strain isolated from a salted small octopus (SC11). The current study aimed to evaluate whether <i>P. pentosaceus</i> (SC11) fermentation could enhance the anti-allergic potential of <i>C. militaris</i> cultured on germinated <i>Rhynchosia nulubilis</i> (GRC) against a type I hypersensitive reaction in in vitro and in vivo studies. Total antioxidant capacity and cordycepin content were significantly increased in GRC after SC11 fermentation. GRC-SC11 showed significantly enhanced anti-allergic responses by inhibiting immunoglobulin E (IgE)/antigen-induced degranulation in RBL-2H3 cells, compared to GRC. The results demonstrated the significant inhibition of phosphorylated spleen tyrosine kinase (Syk)/ p38/GRB2-associated binding protein 2 (Gab2)/c-jun in IgE/Ag-triggered RBL-2H3 cells. Furthermore, suppressed mRNA levels of interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) in IgE/Ag-activated RBL-2H3 cells were observed. GRC-SC11 significantly ameliorated IgE-induced allergic reactions by suppressing the ear swelling, vascular permeability, and inflammatory cell infiltration in passive cutaneous anaphylaxis (PCA) BALB/c mice. In conclusion, GRC fermented with <i>P.pentosaceus</i> exerted enhanced anti-allergic effects, and increased the cordycepin content and antioxidants potential compared to GRC. It can be used as bio-functional food in the prevention and management of type I allergic diseases.https://www.mdpi.com/2072-6643/13/11/3849<i>Cordyceps militaris</i>anti-allergyfermentation |
| spellingShingle | Abdul-Rehman Phull Kyu-Ree Dhong Hye-Jin Park Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model Nutrients <i>Cordyceps militaris</i> anti-allergy fermentation |
| title | Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model |
| title_full | Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model |
| title_fullStr | Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model |
| title_full_unstemmed | Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model |
| title_short | Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model |
| title_sort | lactic acid bacteria fermented i cordyceps militaris i grc sc11 suppresses ige mediated mast cell activation and type i hypersensitive allergic murine model |
| topic | <i>Cordyceps militaris</i> anti-allergy fermentation |
| url | https://www.mdpi.com/2072-6643/13/11/3849 |
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