Analysis of Flavonoids in <i>Dalbergia odorifera</i> by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry
<i>Dalbergia odorifera</i>, a traditional Chinese medicine, has been used to treat cardio- and cerebrovascular diseases in China for thousands of years. Flavonoids are major active compounds in <i>D. odorifera</i>. In this paper, a rapid and sensitive ultra-high performance l...
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MDPI AG
2020-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/2/389 |
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| author | Xiangsheng Zhao Shihui Zhang Dan Liu Meihua Yang Jianhe Wei |
| author_facet | Xiangsheng Zhao Shihui Zhang Dan Liu Meihua Yang Jianhe Wei |
| author_sort | Xiangsheng Zhao |
| collection | DOAJ |
| description | <i>Dalbergia odorifera</i>, a traditional Chinese medicine, has been used to treat cardio- and cerebrovascular diseases in China for thousands of years. Flavonoids are major active compounds in <i>D. odorifera</i>. In this paper, a rapid and sensitive ultra-high performance liquid chromatography-triple quadrupole mass spectrometry method was developed and validated for simultaneous determination of 17 flavonoids in <i>D. odorifera</i>. Quantification was performed by multiple reaction monitoring using electrospray ionization in negative ion mode. Under the optimum conditions, calibration curves for the 17 analytes displayed good linearity (<i>r</i><sup>2</sup> > 0.9980). The intra- and inter-day precisions (relative standard deviations) were lower than 5.0%. The limit of quantitation ranged from 0.256 to 18.840 ng/mL. The mean recovery range at three spiked concentrations was 94.18−101.97%. The validated approach was successfully applied to 18 samples of <i>D. odorifera</i>. Large variation was observed for the contents of the 17 analytes. Sativanone and 3′-<i>O</i>-methylviolanone were the dominant compounds. The fragmentation behaviors of six flavonoids were investigated using UPLC with quadrupole time-of-flight tandem mass spectrometry. In negative ion electrospray ionization mass spectrometry, all the flavonoids yielded prominent [M − H]<sup>−</sup> ions. Fragments for losses of CH<sub>3</sub>, CO, and CO<sub>2</sub> were observed in the mass spectra. Formononetin, liquiritigenin, isoliquiritigenin, sativanone, and alpinetin underwent retro-Diels−Alder fragmentations. The proposed method will be helpful for quality control of <i>D</i><i>. odorifera</i>. |
| first_indexed | 2024-12-13T06:47:59Z |
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| series | Molecules |
| spelling | doaj.art-121e6dcfe58a4345ac71be5447ac86372022-12-21T23:56:13ZengMDPI AGMolecules1420-30492020-01-0125238910.3390/molecules25020389molecules25020389Analysis of Flavonoids in <i>Dalbergia odorifera</i> by Ultra-Performance Liquid Chromatography with Tandem Mass SpectrometryXiangsheng Zhao0Shihui Zhang1Dan Liu2Meihua Yang3Jianhe Wei4Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, ChinaHainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaHainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, ChinaHainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China<i>Dalbergia odorifera</i>, a traditional Chinese medicine, has been used to treat cardio- and cerebrovascular diseases in China for thousands of years. Flavonoids are major active compounds in <i>D. odorifera</i>. In this paper, a rapid and sensitive ultra-high performance liquid chromatography-triple quadrupole mass spectrometry method was developed and validated for simultaneous determination of 17 flavonoids in <i>D. odorifera</i>. Quantification was performed by multiple reaction monitoring using electrospray ionization in negative ion mode. Under the optimum conditions, calibration curves for the 17 analytes displayed good linearity (<i>r</i><sup>2</sup> > 0.9980). The intra- and inter-day precisions (relative standard deviations) were lower than 5.0%. The limit of quantitation ranged from 0.256 to 18.840 ng/mL. The mean recovery range at three spiked concentrations was 94.18−101.97%. The validated approach was successfully applied to 18 samples of <i>D. odorifera</i>. Large variation was observed for the contents of the 17 analytes. Sativanone and 3′-<i>O</i>-methylviolanone were the dominant compounds. The fragmentation behaviors of six flavonoids were investigated using UPLC with quadrupole time-of-flight tandem mass spectrometry. In negative ion electrospray ionization mass spectrometry, all the flavonoids yielded prominent [M − H]<sup>−</sup> ions. Fragments for losses of CH<sub>3</sub>, CO, and CO<sub>2</sub> were observed in the mass spectra. Formononetin, liquiritigenin, isoliquiritigenin, sativanone, and alpinetin underwent retro-Diels−Alder fragmentations. The proposed method will be helpful for quality control of <i>D</i><i>. odorifera</i>.https://www.mdpi.com/1420-3049/25/2/389<i>dalbergia odorifera</i>flavonoidassayfragmentation behavioruplc-ms/ms |
| spellingShingle | Xiangsheng Zhao Shihui Zhang Dan Liu Meihua Yang Jianhe Wei Analysis of Flavonoids in <i>Dalbergia odorifera</i> by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Molecules <i>dalbergia odorifera</i> flavonoid assay fragmentation behavior uplc-ms/ms |
| title | Analysis of Flavonoids in <i>Dalbergia odorifera</i> by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_full | Analysis of Flavonoids in <i>Dalbergia odorifera</i> by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_fullStr | Analysis of Flavonoids in <i>Dalbergia odorifera</i> by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_full_unstemmed | Analysis of Flavonoids in <i>Dalbergia odorifera</i> by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_short | Analysis of Flavonoids in <i>Dalbergia odorifera</i> by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry |
| title_sort | analysis of flavonoids in i dalbergia odorifera i by ultra performance liquid chromatography with tandem mass spectrometry |
| topic | <i>dalbergia odorifera</i> flavonoid assay fragmentation behavior uplc-ms/ms |
| url | https://www.mdpi.com/1420-3049/25/2/389 |
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