The assessment of angiogenesis and fibroblastic stromagenesis in hyperplastic and pre-invasive breast lesions
<p>Abstract</p> <p>Background</p> <p>To investigate the changes of the neoplastic microenvironment during the different morphological alterations of hyperplastic and pre-invasive breast lesions.</p> <p>Methods</p> <p>78 in situ ductal carcinomas...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-04-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/8/88 |
| _version_ | 1818229928834891776 |
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| author | Louvrou Niki Keramopoullos Dimitrios Yiannou Petros Vrekoussis Thomas Messini Irene Pavlakis Kitty Liakakos Theodoros Stathopoulos Efstathios N |
| author_facet | Louvrou Niki Keramopoullos Dimitrios Yiannou Petros Vrekoussis Thomas Messini Irene Pavlakis Kitty Liakakos Theodoros Stathopoulos Efstathios N |
| author_sort | Louvrou Niki |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>To investigate the changes of the neoplastic microenvironment during the different morphological alterations of hyperplastic and pre-invasive breast lesions.</p> <p>Methods</p> <p>78 in situ ductal carcinomas of all degrees of differentiation, 22 atypical ductal hyperplasias, 25 in situ lobular carcinomas, 18 atypical lobular hyperplasias, 32 ductal epithelial hyperplasias of usual type and 8 flat atypias were immunohistochemically investigated for the expression of vascular endothelial growth factor (VEGF), smooth muscle actin (SMA) and CD34, while microvessel density (MVD) was counted using the anti-CD31 antibody.</p> <p>Results</p> <p>VEGF expression was strongly correlated with MVD in all hyperplastic and pre-invasive breast lesions (p < 0.05). Stromagenesis, as characterized by an increase in SMA and a decrease in CD34 positive myofibroblasts was observed mostly around ducts harboring high grade in situ carcinoma and to a lesser extent around moderately differentiated DCIS. In these two groups of in situ carcinomas, a positive correlation between MVD and SMA (p < 0.05) was observed. On the contrary, CD34 was found to be inversely related to MVD (p < 0.05). No statistically significant changes of the stromal fibroblasts were observed in low grade DCIS neither in any of the other lesions under investigation as compared to normal mammary intra- and interlobular stroma.</p> <p>Conclusion</p> <p>Angiogenesis is observed before any significant fibroblastic stromagenesis in pre-invasive breast lesions. A composite phenotype characterized by VEGF positive epithelial cells and SMA positive/CD34 negative stromal cells, is identified mostly in intermediate and high grade DCIS. These findings might imply for new therapeutic strategies using both anti-angiogenic factors and factors selectively targeting tumor stroma in order to prevent the progression of DCIS to invasive carcinoma.</p> |
| first_indexed | 2024-12-12T10:26:24Z |
| format | Article |
| id | doaj.art-12216bfe095b4834a76a773dde414b0e |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-12T10:26:24Z |
| publishDate | 2008-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-12216bfe095b4834a76a773dde414b0e2022-12-22T00:27:27ZengBMCBMC Cancer1471-24072008-04-01818810.1186/1471-2407-8-88The assessment of angiogenesis and fibroblastic stromagenesis in hyperplastic and pre-invasive breast lesionsLouvrou NikiKeramopoullos DimitriosYiannou PetrosVrekoussis ThomasMessini IrenePavlakis KittyLiakakos TheodorosStathopoulos Efstathios N<p>Abstract</p> <p>Background</p> <p>To investigate the changes of the neoplastic microenvironment during the different morphological alterations of hyperplastic and pre-invasive breast lesions.</p> <p>Methods</p> <p>78 in situ ductal carcinomas of all degrees of differentiation, 22 atypical ductal hyperplasias, 25 in situ lobular carcinomas, 18 atypical lobular hyperplasias, 32 ductal epithelial hyperplasias of usual type and 8 flat atypias were immunohistochemically investigated for the expression of vascular endothelial growth factor (VEGF), smooth muscle actin (SMA) and CD34, while microvessel density (MVD) was counted using the anti-CD31 antibody.</p> <p>Results</p> <p>VEGF expression was strongly correlated with MVD in all hyperplastic and pre-invasive breast lesions (p < 0.05). Stromagenesis, as characterized by an increase in SMA and a decrease in CD34 positive myofibroblasts was observed mostly around ducts harboring high grade in situ carcinoma and to a lesser extent around moderately differentiated DCIS. In these two groups of in situ carcinomas, a positive correlation between MVD and SMA (p < 0.05) was observed. On the contrary, CD34 was found to be inversely related to MVD (p < 0.05). No statistically significant changes of the stromal fibroblasts were observed in low grade DCIS neither in any of the other lesions under investigation as compared to normal mammary intra- and interlobular stroma.</p> <p>Conclusion</p> <p>Angiogenesis is observed before any significant fibroblastic stromagenesis in pre-invasive breast lesions. A composite phenotype characterized by VEGF positive epithelial cells and SMA positive/CD34 negative stromal cells, is identified mostly in intermediate and high grade DCIS. These findings might imply for new therapeutic strategies using both anti-angiogenic factors and factors selectively targeting tumor stroma in order to prevent the progression of DCIS to invasive carcinoma.</p>http://www.biomedcentral.com/1471-2407/8/88 |
| spellingShingle | Louvrou Niki Keramopoullos Dimitrios Yiannou Petros Vrekoussis Thomas Messini Irene Pavlakis Kitty Liakakos Theodoros Stathopoulos Efstathios N The assessment of angiogenesis and fibroblastic stromagenesis in hyperplastic and pre-invasive breast lesions BMC Cancer |
| title | The assessment of angiogenesis and fibroblastic stromagenesis in hyperplastic and pre-invasive breast lesions |
| title_full | The assessment of angiogenesis and fibroblastic stromagenesis in hyperplastic and pre-invasive breast lesions |
| title_fullStr | The assessment of angiogenesis and fibroblastic stromagenesis in hyperplastic and pre-invasive breast lesions |
| title_full_unstemmed | The assessment of angiogenesis and fibroblastic stromagenesis in hyperplastic and pre-invasive breast lesions |
| title_short | The assessment of angiogenesis and fibroblastic stromagenesis in hyperplastic and pre-invasive breast lesions |
| title_sort | assessment of angiogenesis and fibroblastic stromagenesis in hyperplastic and pre invasive breast lesions |
| url | http://www.biomedcentral.com/1471-2407/8/88 |
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