The septate junction component bark beetle is required for Drosophila intestinal barrier function and homeostasis
Summary: Age-related loss of intestinal barrier function has been documented across species, but the causes remain unknown. The intestinal barrier is maintained by tight junctions (TJs) in mammals and septate junctions (SJs) in insects. Specialized TJs/SJs, called tricellular junctions (TCJs), are l...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223009781 |
| _version_ | 1797806346977935360 |
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| author | Rachel A. Hodge Mirna Ghannam Emma Edmond Fernando de la Torre Cecilia D’Alterio Nida Hatice Kaya Martin Resnik-Docampo Tobias Reiff D. Leanne Jones |
| author_facet | Rachel A. Hodge Mirna Ghannam Emma Edmond Fernando de la Torre Cecilia D’Alterio Nida Hatice Kaya Martin Resnik-Docampo Tobias Reiff D. Leanne Jones |
| author_sort | Rachel A. Hodge |
| collection | DOAJ |
| description | Summary: Age-related loss of intestinal barrier function has been documented across species, but the causes remain unknown. The intestinal barrier is maintained by tight junctions (TJs) in mammals and septate junctions (SJs) in insects. Specialized TJs/SJs, called tricellular junctions (TCJs), are located at the nexus of three adjacent cells, and we have shown that aging results in changes to TCJs in intestines of adult Drosophila melanogaster. We now demonstrate that localization of the TCJ protein bark beetle (Bark) decreases in aged flies. Depletion of bark from enterocytes in young flies led to hallmarks of intestinal aging and shortened lifespan, whereas depletion of bark in progenitor cells reduced Notch activity, biasing differentiation toward the secretory lineage. Our data implicate Bark in EC maturation and maintenance of intestinal barrier integrity. Understanding the assembly and maintenance of TCJs to ensure barrier integrity may lead to strategies to improve tissue integrity when function is compromised. |
| first_indexed | 2024-03-13T06:05:58Z |
| format | Article |
| id | doaj.art-1223f9d0000b4aaabf9a03cb56bfcd4a |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-13T06:05:58Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-1223f9d0000b4aaabf9a03cb56bfcd4a2023-06-12T04:09:26ZengElsevieriScience2589-00422023-06-01266106901The septate junction component bark beetle is required for Drosophila intestinal barrier function and homeostasisRachel A. Hodge0Mirna Ghannam1Emma Edmond2Fernando de la Torre3Cecilia D’Alterio4Nida Hatice Kaya5Martin Resnik-Docampo6Tobias Reiff7D. Leanne Jones8Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USAInstitute of Genetics, Heinrich-Heine-University, Düsseldorf, GermanyDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USADepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USAInstitute of Genetics, Heinrich-Heine-University, Düsseldorf, GermanyDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USAInstitute of Genetics, Heinrich-Heine-University, Düsseldorf, GermanyDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Anatomy, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Medicine, Division of Geriatrics, University of California, San Francisco, San Francisco, CA 94143, USA; Bakar Aging Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA; Corresponding authorSummary: Age-related loss of intestinal barrier function has been documented across species, but the causes remain unknown. The intestinal barrier is maintained by tight junctions (TJs) in mammals and septate junctions (SJs) in insects. Specialized TJs/SJs, called tricellular junctions (TCJs), are located at the nexus of three adjacent cells, and we have shown that aging results in changes to TCJs in intestines of adult Drosophila melanogaster. We now demonstrate that localization of the TCJ protein bark beetle (Bark) decreases in aged flies. Depletion of bark from enterocytes in young flies led to hallmarks of intestinal aging and shortened lifespan, whereas depletion of bark in progenitor cells reduced Notch activity, biasing differentiation toward the secretory lineage. Our data implicate Bark in EC maturation and maintenance of intestinal barrier integrity. Understanding the assembly and maintenance of TCJs to ensure barrier integrity may lead to strategies to improve tissue integrity when function is compromised.http://www.sciencedirect.com/science/article/pii/S2589004223009781EntomologyMolecular biologyCell biology |
| spellingShingle | Rachel A. Hodge Mirna Ghannam Emma Edmond Fernando de la Torre Cecilia D’Alterio Nida Hatice Kaya Martin Resnik-Docampo Tobias Reiff D. Leanne Jones The septate junction component bark beetle is required for Drosophila intestinal barrier function and homeostasis iScience Entomology Molecular biology Cell biology |
| title | The septate junction component bark beetle is required for Drosophila intestinal barrier function and homeostasis |
| title_full | The septate junction component bark beetle is required for Drosophila intestinal barrier function and homeostasis |
| title_fullStr | The septate junction component bark beetle is required for Drosophila intestinal barrier function and homeostasis |
| title_full_unstemmed | The septate junction component bark beetle is required for Drosophila intestinal barrier function and homeostasis |
| title_short | The septate junction component bark beetle is required for Drosophila intestinal barrier function and homeostasis |
| title_sort | septate junction component bark beetle is required for drosophila intestinal barrier function and homeostasis |
| topic | Entomology Molecular biology Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223009781 |
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